赫赛汀诱导子宫内膜癌Ishikawa细胞凋亡并增强其对化疗的敏感性

发布时间：2018-06-17 09:05

本文选题：赫赛汀 + 子宫内膜癌　；参考：《中国病理生理杂志》2015年09期

【摘要】：目的:研究赫赛汀单独或联合阿霉素(adriamycin,ADR)、顺铂(cisplatin,DDP)及紫杉醇(paclitaxel,PTX)对子宫内膜癌细胞凋亡和化疗敏感性的影响,为临床应用赫赛汀治疗子宫内膜癌提供理论依据。方法:MTT法检测赫赛汀、ADR、DDP及PTX处理子宫内膜癌Ishikawa细胞的IC50。进一步应用1/2 IC50量的赫赛汀与各1/2 IC50量的ADR、DDP及PTX药物联合,流式细胞术检测细胞周期与凋亡变化。结果:赫赛汀抑制子宫内膜癌Ishikawa细胞生长,引起细胞G1期阻滞,诱导细胞凋亡。子宫内膜癌Ishikawa细胞应用赫赛汀、ADR、DDP及PTX的IC50分别为57.12 mg/L、0.572μmol/L、67.4μmol/L和719.5 nmol/L,赫赛汀联合化疗后显著提高各组化疗药的杀伤效果,诱导细胞凋亡,与单纯化疗组相比差异有统计学意义(P0.05)。结论:赫赛汀诱导子宫内膜癌细胞凋亡,并提高子宫内膜癌细胞株对化疗的敏感性。
[Abstract]:Objective: to study the effects of Herceptin alone or in combination with adriamycin (ADRN) and paclitaxellol (PTX) on apoptosis and chemosensitivity of endometrial cancer cells, and to provide a theoretical basis for the clinical application of Herceptin in the treatment of endometrial carcinoma. Methods the IC50 of endometrial carcinoma Ishikawa cells was detected by 10% MTT assay with ADRDP and PTX. The cell cycle and apoptosis were detected by flow cytometry with 1 / 2 IC50 Herceptin combined with 1 / 2 IC50 of ADRDDP and PTX. Results: Herceptin inhibited the growth of endometrial carcinoma Ishikawa cells and induced G1 phase arrest and apoptosis. The IC50 of Ishikawa cells treated with ADRDP and PTX were 57.12 mg / L, 0.572 渭 mol / L and 719.5 nmol / L, respectively. Herceptin combined with chemotherapy significantly increased the killing effect and induced apoptosis of the cells compared with the chemotherapy group (P 0.05). Conclusion: Herceptin induces apoptosis of endometrial cancer cells and increases the sensitivity of endometrial cancer cells to chemotherapy.
【作者单位】：暨南大学附属第一医院妇产科;南方医科大学附属南方医院妇产科;
【基金】：广东省科技计划(No.2013B021800163) 暨南大学第一临床医学院科研培育专项基金资助项目(No.2014102)
【分类号】：R737.33

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