PRL-3对子宫内膜异位症患者内膜细胞迁移能力的影响及雌、孕激素对其调控的相关性研究

发布时间：2018-06-18 17:44

本文选题：子宫内膜异位症 + 迁移能力　；参考：《中南大学》2014年硕士论文

【摘要】：目的本课题通过原代培养正常人及子宫内膜异位症患者子宫内膜细胞,比较不同细胞的形态学结构及迁移能力的差别。通过给予不同细胞雌、孕激素及原矾酸钠(SoV)干预,测定肝再生磷酸酶3(phosphatase of regenerating liver-3, PRL-3)的表达情况及变化,探讨PRL-3与子宫内膜细胞迁徙能力差异的关系,研究子宫内膜异位症高转移高侵袭的发病机制。方法 1.原代培养正常人及子宫内膜异位症患者子宫内膜细胞,比较不同细胞的形态学结构。 2.用划线实验比较不同细胞的迁移能力差别。 3. qT-PCR法及Western Blot法测定不同细胞中PRL-3的表达。 4.予以PRL-3抑制剂原矾酸钠、雌、孕激素干预后,行划线实验确定细胞迁移能力变化。qT-PCR法及Western Blot法测定不同细胞中PRL-3的表达。结果 1.子宫内膜细胞由腺上皮细胞及间质细胞组成,腺上皮细胞角蛋白免疫组化染色呈阳性反应,间质细胞波形蛋白免疫组化染色呈阳性反应。随着传代次数增加,角蛋白阳性细胞数量越来越少,波形蛋白阳性细胞所占比例占绝大部分,为主要细胞。 2.划线实验结果显示：在位内膜细胞在36小时后细胞迁移的距离大于正常子宫内膜细胞迁移的距离；对照组内膜细胞迁移的距离大于加入0.5umol/l SoV组细胞迁移的距离；10-6mol/l雌二醇组细胞迁移的距离大于对照组细胞迁移的距离。对照组内膜细胞迁移的距离大于10-5mol/l孕酮细胞迁移的距离。 3.PRL-3在EMs患者在位内膜上皮细胞中表达强于正常人细胞中的表达,两者之间差异有统计学意义(P0.05)；对照组细胞中PRL-3表达强于加入SoV组细胞中表达,两者之间差异有统计学意义(P0.05)；加入雌激素组细胞中PRL-3表达强于对照组细胞中表达,两者之间差异有统计学意义(P0.05)；对照组细胞中PRL-3表达强于加入孕激素组细胞中的表达,两者之间差异有统计学意义(P0.05) 结论 1.EMs患者在位子宫内膜细胞迁移能力强于正常人子宫内膜细胞迁移能力,PRL-3在EMs在位子宫内膜细胞中表达强于正常人子宫内膜细胞,子宫内膜细胞可能通过PRL-3促进子宫内膜细胞的迁移能力。 2.雌激素可能通过上调PRL-3表达,正性调节子宫内膜细胞的迁移。孕激素可能通过下调PRL-3表达,负性调节子宫内膜细胞的迁移。 3.SoV能通过抑制PRL-3的表达,抑制子宫内膜细胞的迁移能力。
[Abstract]:Purpose

In order to study the relationship between PRL - 3 and migration ability of endometrial cells , the relationship between PRL - 3 and migration ability of endometrial cells was determined by the intervention of estrogen , progesterone and sodium sulfate ( SoV ) in different cells .

method

1 . The endometrial cells of normal and endometriosis were cultured in primary culture , and the morphological structure of different cells was compared .

2 . The difference of migration ability of different cells was compared by scoring experiment .

3 . The expression of PRL - 3 in different cells was determined by qT - PCR and Western Blot .

4 . The expression of PRL - 3 in different cells was determined by the method of qT - PCR and Western Blot .

Results

1 . The endometrial cells were composed of glandular epithelial cells and stroma cells . The cytokeratins of the glandular epithelial cells showed a positive reaction with immunohistochemical staining . The number of keratin - positive cells decreased with the increase of the number of passages , and the percentage of the positive cells of the protein - positive cells accounted for most of the cells , which were the main cells .

2 . The results showed that the migration distance of the cells after 36 hours was greater than that of normal endometrial cells .
The migration of endometrial cells in the control group was greater than that in the group of 0.5mol / l SoV group .
The distance of migration of cells from 10 - 6 mol / l estradiol group was greater than that of control group .

3 . The expression of PRL - 3 in the epithelial cells of endometriosis was significantly higher than that in normal human cells ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .
PRL - 3 expression in estrogen group was stronger than that in control group ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .

Conclusion

1 . In endometriosis , the migration ability of endometrium cells was stronger than that of normal controls , PRL - 3 expression was stronger than normal endometrium cells in endometriosis , and the migration ability of endometrial cells could be promoted by PRL - 3 .

2 . Estrogen may regulate the migration of endometrial cells by up - regulating the expression of PRL - 3 , and progesterone may regulate the migration of endometrial cells by downregulating PRL - 3 expression .

3 . SoV can inhibit the migration of endometrial cells by inhibiting the expression of PRL - 3 .
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

【参考文献】