壳聚糖凝胶用于避孕的机制及可行性研究

发布时间：2018-06-20 15:35

本文选题：壳聚糖凝胶 + 精子活力　；参考：《青岛大学》2016年硕士论文

【摘要】：目的研究壳聚糖凝胶对精子活力的影响及其影响机制并初步探讨壳聚糖凝胶用于避孕的可行性。方法将200例精液常规在正常范围的精液标本随机分为两组,每组各100例,对照组无任何处理,壳聚糖凝胶组加入壳聚糖凝胶。分别测定并比较两组中活动力为a级(快速向前运动)与b级(慢速或呆滞的前向运动)的精子百分率;形态学正常、头部缺陷、体部缺陷及尾部缺陷的精子数;精子顶体酶活性的定量值;精浆酸性磷酸酶、中性α-葡糖苷酶及锌的含量。结果壳聚糖凝胶组精子活动力较对照组显著降低,两组活动力为a级的精子百分率差异有统计学意义(P0.05),活动力为b级的精子百分率差异无统计学意义(P0.05)。壳聚糖凝胶组畸形精子数较对照组显著增加,两组形态学正常、头部缺陷尤其是尾部缺陷的精子数差异均有统计学意义(P0.05),而中段缺陷的精子数差异无统计学意义(P0.05)。壳聚糖凝胶组精子顶体酶活性的定量值较对照组降低,但差异无统计学意义(P0.05)。壳聚糖凝胶组精浆酸性磷酸酶、中性α-葡糖苷酶及锌的含量均较对照组明显降低,差异均有统计学意义(P0.05)。结论壳聚糖凝胶可显著降低精子的活动力并致精子畸形,同时引起精浆各生化指标不同程度地降低,因而具有显著的体外杀精作用,值得进一步研究、开发为一种新型的杀精剂类外用避孕药物。
[Abstract]:Objective to study the effect of chitosan gel on sperm motility and its mechanism and to explore the feasibility of using chitosan gel for contraception. Methods 200 semen samples were randomly divided into two groups, 100 cases in each group. The control group had no treatment, and the chitosan gel group was added with chitosan gel. The percentage of spermatozoa in the two groups were measured and compared, the sperm numbers of normal morphology, head defect, body defect and tail defect were measured and compared between the two groups. The quantitative value of acrosin activity in spermatozoa, the content of acid phosphatase, neutral 伪 -glucosidase and zinc in seminal plasma. Results the sperm motility of the chitosan gel group was significantly lower than that of the control group. There was a significant difference in the percentage of spermatozoa with a grade a between the two groups, but there was no significant difference in the percentage of the spermatozoa with the b grade in the two groups. The number of abnormal sperm in chitosan gel group was significantly higher than that in control group. The morphology of the two groups was normal. The difference of sperm number in head defect, especially in tail defect was statistically significant (P 0.05), but there was no significant difference in sperm count in middle segment (P 0.05). The quantitative value of acrosin activity in chitosan gel group was lower than that in control group, but the difference was not statistically significant (P 0.05). The contents of acid phosphatase, neutral 伪 -glucosidase and zinc in the seminal plasma of chitosan gel group were significantly lower than those in the control group (P 0.05). Conclusion chitosan gel can significantly reduce the motility of sperm and cause sperm deformity, at the same time, the biochemical indexes of seminal plasma can be decreased to some extent, so it has remarkable spermicidal effect in vitro, which is worthy of further study. To develop a new spermicide for external use.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R169.41

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