子宫内膜异位症盆腔粘连大鼠模型的建立与评价

发布时间：2018-06-22 22:19

本文选题：子宫内膜异位症 + 盆腔粘连　；参考：《中国比较医学杂志》2017年12期

【摘要】：目的探索子宫内膜异位症盆腔粘连大鼠模型的建立方法及评价指标。方法采用自体移植法将子宫内膜移植于大鼠肠系膜间,术后1、3、5、7、14、21、28 d分别随机抽取8只大鼠开腹观察病灶生长情况,采用美国生殖学会粘连分级(AFS)分级、Haber粘连评分两种方法同时对盆腔粘连程度进行评分;同时采取各组病灶及周围粘连腹膜组织行HE染色;术后5、7、14、21、28 d组分别采取病灶周围粘连组织,对其组织型纤溶酶原激活剂(t PA)含量进行动态检测。结果两种粘连评分方法均显示造模后5 d已形成典型的盆腔粘连;与空白对照组及假手术相比,造模5 d后各模型组大鼠盆腔粘连腹膜组织中t PA含量极度降低(P0.01),造模28 d后t PA含量有上升趋势,但仍显著低于空白对照组及假手术组(P0.01)。结论采用自体移植法将子宫内膜移植于肠系膜间建立EMs盆腔粘连模型,造模术后5 d即为成熟模型,t PA与EMs盆腔粘连的发生具有相关性。
[Abstract]:Objective to explore the method and evaluation index of pelvic adhesion model of endometriosis in rats. Methods the endometrium was transplanted into mesenteric mesentery of rats by autologous transplantation. Eight rats were randomly selected to observe the growth of the mesentery on the 28th day after operation. The degree of pelvic adhesions was evaluated by the American Society of Reproduction Association (AFS) grade and Haber's adhesion grade, and HE staining was used in each group of lesions and surrounding adhesion peritoneum tissue at the same time. The tissue type plasminogen activator (t PA) content was measured dynamically in the postoperative group of 28 days after operation with adherent tissue around the lesion. Results the two adhesion scoring methods showed that typical pelvic adhesions had been formed 5 days after modeling, compared with blank control group and sham operation. After 5 days, the content of t-PA in the peritoneal tissue of the rats with pelvic adhesions was extremely decreased (P0.01), and the content of t-PA increased after 28 days, but still significantly lower than that of the blank control group and sham-operation group (P0.01). Conclusion the pelvic adhesion model of endometrium was established by autologous transplantation of endometrium into mesentery. The model is mature model 5 days after operation. There is a correlation between t PA and pelvic adhesion.
【作者单位】：中国中医科学院广安门医院;北京市回民医院;
【基金】：国家自然科学基金资助项目(81373678)
【分类号】：R-332;R711.71

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