葡萄糖转运蛋白2和蛋白酪氨酸磷酸酶-1B在甲状腺毒症妊娠大鼠胰腺中表达的观察

发布时间：2018-06-23 16:09

  本文选题：甲状腺毒症 + 妊娠　； 参考：《中国糖尿病杂志》2015年05期

【摘要】：目的检测甲状腺毒症妊娠大鼠胰腺中葡萄糖转运蛋白2(GLUT2)和蛋白酪氨酸磷酸酶-1B(PTP-1B)的表达,探讨其影响糖代谢的可能机制。方法雌性SD大鼠分为非妊娠组和待妊娠组,待妊娠组按雌雄比例2∶1合笼受孕后进一步分为单纯妊娠(R)组、妊娠期左甲状腺素钠低剂量(RA)组、妊娠期左甲状腺素钠中剂量(RB)组和妊娠期左甲状腺素钠高剂量(RC)组,非妊娠组进一步分为正常对照(N)组、左甲状腺素钠低剂量(A)组、左甲状腺素钠中剂量(B)组和左甲状腺素钠高剂量(C)组。予A、RA组(左甲状腺素钠50μg/100g)、B、RB组(左甲状腺素钠100μg/100g)、C、RC组(左甲状腺素钠150μg/100g)灌胃制造甲状腺毒症模型,N、R组予等量生理盐水。检测各组雌鼠甲状腺功能、FPG和FIns,Western blot检测胰腺中GLUT2和PTP-1B的表达。结果随着左甲状腺素钠剂量增加,N、A、B、C组和R、RA、RB、RC组游离三碘甲状腺原氨酸(FT3)[(2.65±0.07)vs(4.05±0.10)vs(4.87±0.63)vs(5.62±0.73)pmol/L;(2.67±0.10)vs(4.07±0.09)vs(4.84±0.11)vs(5.65±0.18)pmol/L,P0.01]、游离甲状腺素(FT4)[(10.54±0.58)vs(13.46±0.78)vs(25.77±0.85)vs(36.58±0.91)pmol/L;(10.61±0.79)vs(13.53±0.66)vs(25.90±1.12)vs(36.65±0.62)pmol/L,P0.01]升高,促甲状腺激素(TSH)[(0.086±0.009)vs(0.029±0.002)vs(0.024±0.001)vs(0.018±0.002)μU/ml;(0.070±0.009)vs(0.027±0.002)vs(0.021±0.001)vs(0.015±0.002)μU/ml,P0.01]降低。甲状腺毒症各组(A、B、C组和RA、RB、RC组)FPG较对照组(N组和R组)升高[(5.43±0.32)、(5.74±0.21)、(6.34±0.46)vs(4.98±0.25)mmol/L;(5.37±0.48)、(5.78±0.39)、(6.45±0.44)vs(4.88±0.38)mmol/L,P0.01],C组FIns较N组升高[(10.40±1.52)vs(8.25±0.80)μU/ml,P0.01],妊娠各组(R、RA、RB、RC组)FIns较非妊娠各组(N、A、B、C组)升高[(9.16±1.28)vs(8.25±0.80)μU/ml;(10.40±1.31)vs(9.20±1.32)μU/ml;(10.41±1.43)vs(9.75±1.24)μU/ml;(11.09±1.17)vs(10.40±1.52)μU/ml,P0.01]。Western blot结果显示,甲状腺毒症各组GLUT2表达较N、R组降低,PTP-1B表达升高(P0.05)。结论 GLUT2及PTP-1B可能与妊娠期合并甲状腺毒症雌鼠出现糖代谢异常相关。
[Abstract]:Objective to detect the expression of glucose transporter 2 (GLUT2) and protein tyrosine phosphatase 1B (PTP-1B) in the pancreas of pregnant rats with thyroid toxicity, and to explore the possible mechanism of the effects of GLUT2 and PTP-1B on glucose metabolism. Methods female Sprague-Dawley rats were divided into two groups: the non-pregnant group and the unpregnant group. The female SD rats were further divided into simple pregnancy (R) group and low dose of levothyroxine sodium (RA) group according to the sex ratio of 2:1. The middle dose of levothyroxine sodium (RB) during pregnancy and the high dose of levothyroxine sodium during pregnancy (RC) group were further divided into normal control (N) group and low-dose (A) group. Middle dose (B) group and high dose (C) group of levothyroxine sodium. The model of thyrotoxicosis was made by oral administration of Thyroxine sodium (50 渭 g / 100 g) in Acara group (50 渭 g / 100 g left thyroxine sodium) and 100 渭 g / 100 g left thyroxine sodium group (100 渭 g / 100 g left thyroxine sodium group) and the same amount of normal saline was given to the control group (150 渭 g / 100g levathyroxine sodium). The expression of GLUT2 and PTP-1B in pancreas was detected by Western blot. 缁撴灉闅忕潃宸︾敳鐘惰吅绱犻挔鍓傞噺澧炲姞,N,A,B,C缁勫拰R,RA,RB,RC缁勬父绂讳笁纰樼敳鐘惰吅鍘熸皑閰，

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