氧化苦参碱对人宫颈癌SiHa细胞株增殖及凋亡的影响

发布时间：2018-06-28 12:11

  本文选题：氧化苦参碱 + SiHa细胞株　； 参考：《世界科技研究与发展》2014年03期

【摘要】：目的研究氧化苦参碱对体外人宫颈癌SiHa细胞株增殖活性及凋亡的影响。方法对人宫颈癌SiHa细胞株进行体外培养,经氧化苦参碱处理的细胞组为实验组,未经处理组为对照组。采用MTT细胞存活实验检测氧化苦参碱对入宫颈鳞癌SiHa细胞的增殖影响,并计算IC50;倒置相差显微镜下观察不同浓度的氧化苦参碱作用48h后SiHa细胞的形态改变;Hoechst33258染色法和Western blot法检测氧化苦参碱对SiHa细胞核及凋亡相关蛋白(P53、Bax及Bcl-2)表达水平的影响。结果 MTT结果显示氧化苦参碱剂量-时间依耐性抑制人宫颈癌SiHa细胞的体外增殖(P0.05),计算24 h、48 h和72 h的IC50分别为(1 028.41±3.57)μg/ml、(701.72±6.01)μg/ml和(406.88±2.15)μg/ml;氧化苦参碱处理48 h后,SiHa细胞的形态特征及数目发生显著变化,且随氧化苦参碱浓度的增加而愈加明显;Hoechst33258染色证实氧化苦参碱处理后SiHa细胞发生凋亡,可见典型的凋亡特征;Western blot结果证实氧化苦参碱(700μg/ml、1 600μg/ml)处理48 h后,和对照组比较,药物处理组细胞凋亡周期蛋白P53、Bax表达上调(P0.05),而Bcl-2表达下调(P0.05),Bax/Bcl-2的比率明显增加(P0.05)。结论氧化苦参碱可抑制体外人宫颈癌SiHa细胞的体外增殖活性发挥其抗肿瘤作用,其作用机制可能诱导SiHa细胞的凋亡有关。
[Abstract]:Objective to study the effect of oxymatrine on proliferation and apoptosis of human cervical cancer cell line SIHA in vitro. Methods Human cervical cancer SIHA cells were cultured in vitro. The cells treated with oxymatrine were treated as experimental group and untreated group as control group. MTT cell survival assay was used to detect the effect of oxymatrine on the proliferation of SIHA cells in cervical squamous cell carcinoma. The morphologic changes of SIHA cells treated with different concentrations of oxymatrine for 48 h were observed. Hoechst33258 staining and Western blot were used to detect the effect of oxymatrine on the expression of nuclear and apoptosis-related proteins (P53, Bax and Bcl-2) in SIHA cells. Results MTT results showed that oxymatrine inhibited the proliferation of human cervical cancer SIHA cells in vitro according to tolerance (P0.05). The IC50 of SIHA cells at 24 h for 48 h and 72 h were (1 028.41 卤3.57) 渭 g / ml, (701.72 卤6.01) 渭 g/ml and (406.88 卤2.15) 渭 g / ml, respectively. Signs and numbers have changed significantly, With the increase of oxymatrine concentration, Hoechst33258 staining proved that apoptosis occurred in SIHA cells after treated with oxymatrine. The typical apoptotic characteristics of SIHA cells were confirmed by Western blot. The results showed that Oxymatrine (700 渭 g / ml, 1 600 渭 g/ml) was treated for 48 h, compared with the control group. In drug treated group, the expression of apoptosis cyclin P53 + Bax was up-regulated (P0.05), while the expression of Bcl-2 was down-regulated (P0.05) and the ratio of Bax / Bax was significantly increased (P0.05). Conclusion Oxymatrine can inhibit the proliferation activity of human cervical carcinoma SIHA cells in vitro and play an antitumor role, and its mechanism may be related to the apoptosis of SIHA cells.
【作者单位】： 兰州大学第二临床医学院;兰州大学第二医院妇科;上海市浦东新区周浦医院;
【分类号】：R273

【参考文献】

相关期刊论文 前2条

1 马怡红,吴秉铨,柳剑英,由江峰,崔湘霖,王洁良,惠培;三种鼠不同结构p53minigene瞬时转染H1299细胞对其生长的影响[J];中国肺癌杂志;2001年02期

2 朱晓伟;宝金荣;布仁;;苦参碱和氧化苦参碱抗肿瘤作用机制研究进展[J];化学试剂;2010年01期

【共引文献】

相关期刊论文 前10条

1 朱咏梅;钟进义;张丽华;;论著海生多糖肽对辐射诱发小鼠肝细胞POD和Caspase-3异常表达的影响[J];癌变.畸变.突变;2006年04期

2 徐佳;张引娟;鹿刚;单保恩;;STAT3基因RNAi诱导肝癌细胞凋亡及对STAT3信号转导相关基因表达的影响[J];癌变.畸变.突变;2009年05期

3 朱国旗;汤立建;王磊;苏婧婧;汪宁;彭代银;李庆林;;黄芩苷诱导人乳腺癌细胞株MDA-MB-231凋亡作用的研究[J];安徽中医学院学报;2008年02期

4 赵欣,宋今丹;克霉唑诱导人结肠癌细胞CCL229凋亡的实验研究[J];癌症;2002年06期

5 张明生,袁宏银,熊斌,邓清,唐志佼,夏东;IGF-Ⅱ mRNA、bcl-2蛋白在结肠直肠腺癌组织中的表达及临床意义[J];癌症;2002年11期

6 李锦全,陈瑞川,蔡克瑕,叶志云;核黄素光化学反应诱导人胃癌细胞凋亡[J];癌症;2003年03期

7 梁统,陈美s，

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