子宫内膜异位症相关性不孕卵泡液代谢物组初筛及其与卵母细胞质量的关系

发布时间：2018-06-28 12:53

本文选题：子宫内膜异位症(EMS) + 不孕症　；参考：《中华生殖与避孕杂志》2017年07期

【摘要】：目的探讨与子宫内膜异位症(EMS)相关性不孕患者卵母细胞发育潜能相关的代谢物组。方法利用超高效液相色谱-质谱(HPLC-MS)对接受体外受精(IVF)的EMS相关性不孕患者即EMS组(n=27)和同期因男方因素行体外受精的健康妇女即对照组(n=28)进行卵泡液代谢组学分析。结果共观察到与EMS相关性不孕密切相关的差异代谢物28种,主要有脂类、核苷酸类、蛋白降解片段及其它等四大类,其中上调的代谢物主要有Valyl-Arginine、Methyl-15-cyanopentadecanoate、4-Oxo-13-cis-retinoate等15种;下调的代谢物主要有Menadiol dibutyrate、3-hydroxynonanoyl carnitine、Phytosphingosine等13种。结论本研究发现并描绘出两组卵泡液差异性代谢物谱,这28种代谢物组可能成为预测EMS妇女卵母细胞早期发育潜能的重要生物标志物。然而,并未见其与随后的胚胎质量、累积妊娠率以及活产率间明显的相关性。
[Abstract]:Objective to investigate the metabolites associated with oocyte development potential in infertile women with endometriosis (EMS). Methods Ultra-high performance liquid chromatography-mass spectrometry (HPLC-MS) was used to analyze the metabolism of follicular fluid in EMS associated infertility patients (EMS group 27) and healthy women (control group 28) who underwent IVF in vitro fertilization. Results there were 28 different metabolites closely related to EMS related infertility, including lipid, nucleotides, protein degradation fragments and other four main metabolites. The up-regulated metabolites were Valyl-ArginineMethyl-15-cyanopentadecanoate 4-ciso-13-retinoate. The down-regulated metabolites were mainly Menadiol dibutyratea 3-hydroxynonanoyl carnitine and 13 species of Phytosphingosine. Conclusion the different metabolites spectrum of follicular fluid between the two groups were found and depicted in this study. These 28 metabolites may be important biomarkers for predicting the early development potential of EMS women oocytes. However, there was no significant correlation between the later embryo quality, cumulative pregnancy rate, and live birth rate.
【作者单位】：山东中医药大学;山东中医药大学附属医院;
【基金】：国家自然科学基金(面上项目)(81373676,81674018)~~
【分类号】：R711.6;R711.71

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