CDKAL1基因多态性与妊娠期糖尿病及其临床特点的相关性研究

发布时间：2018-06-29 17:46

本文选题：糖尿病 + 妊娠　；参考：《中国糖尿病杂志》2015年06期

【摘要】：目的探讨我国南方人群中CDKAL1基因两个SNP位点rs7754840和rs10448033的多态性与GDM易感性的关系。方法采用病例对照研究,分别选取GDM患者(GDM组)153例和正常糖耐量的孕妇(GNGT组)180名。采用聚合酶链反应-限制性片断长度多态性分析法(PCR-RFLP)测定两个SNP位点多态性分布,并进行统计学分析。结果 GDM组孕前BMI、FPG、TG均高于GNGT组,TC、HDL-C及LDL-C均低于GNGT组(P0.05)。SNP位点rs7754840基因型(CC,GC,GG)频率和等位基因频率与GNGT组比较,差异均无统计学意义(P=0.937、0.933);而SNP位点rs10440833基因型(AA,AT,TT)与GNGT组比较,差异有统计学意义(P=0.024),且风险基因型TT的频率高于GNGT组(TTvs TC+CC,P=0.032);GDM组A等位基因频率高于GNGT组[OR(95%CI):1.519(1.114~2.071),P=0.008];AA基因型患者的FPG高于其他基因型(P=0.011),TG高于TT基因型(P=0.007),HDL-C低于TT基因型(P=0.001)。结论 CDKAL1基因SNP位点rs7754840与GDM无相关性;SNP位点rs10440833与GDM的易感性相关,且该位点多态性可能与血糖、TG及LDL-C的水平有关。
[Abstract]:Objective to investigate the relationship between the polymorphism of rs7754840 and rs10448033 at two SNPs of CDKAL1 gene and the susceptibility of CDKAL1 gene in southern China. Methods A case-control study was conducted in 153 GDM patients and 180 normal glucose tolerance pregnant women (GNGT group). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism distribution of two SNP loci. Results the levels of rs7754840 genotype and allele frequency of rs7754840 gene at SNP locus in GDM group were significantly higher than those in GNGT group (P 0.05), but there was no significant difference between GDM group and GNGT group (P0. 9379. 933), but there was no significant difference between rs10440833 genotype at SNP locus and GNGT group (P < 0. 05), and there was no significant difference in the frequency of rs7754840 genotype (CCG) and allele frequency between GNGT group (P 0. 05) and GNGT group (P 0. 9379. 933). The frequency of A allele in GDM group was higher than that in GNGT group [OR (95CI): 1.519 (1.1142.071) P0. 008] the FPG of AA genotype was higher than that of other genotype (P0. 011) TG was higher than that of TT genotype (P0. 007) HDL-C was lower than that of TT genotype (P0. 001), and the frequency of FPG of AA genotype was higher than that of GNGT group (TTV vs TC CCP0. 032) and the frequency of allele A in GDM group was higher than that in GNGT group [OR (95CI): 1. 519 (1. 114 卤2. 071) P0. 008]. Conclusion there is no correlation between rs7754840 of CDKAL1 gene and rs10440833 of GDM. The polymorphism of SNP locus may be related to the levels of TG and LDL-C of CDKAL1 gene.
【作者单位】：湖北民族学院附属民大医院妇产科;
【分类号】：R714.258

【共引文献】