CCL2通过抑制白细胞介素-24表达增强人绒毛膜滋养细胞株的细胞活力

发布时间：2018-06-30 03:41

本文选题：白细胞介素-(IL-) + CCL　；参考：《中华生殖与避孕杂志》2017年08期

【摘要】：目的探讨白细胞介素-24(interleukin-24,IL-24)对人滋养细胞活力的调节作用。方法免疫组织化学法检测IL-24及其受体(IL-20R1、IL-20R2和IL-22R1)在正常早孕期绒毛组织的表达;In-cell Western法分析重组胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)和趋化因子CCL2对人绒毛膜滋养细胞株HTR-8/SVneo细胞中IL-24表达的影响;MTT法分析重组IL-24和CCL2对HTR-8/SVneo细胞活力的影响。结果 IL-24及其受体均在正常早孕期绒毛组织滋养细胞中强阳性表达。与对照组相比,重组CCL2体外刺激后HTR-8/SVneo细胞IL-24表达水平显著下降(P0.001)。重组IL-24处理后,HTR-8/SVneo细胞活力显著降低(P0.05或P0.001);相反,IL-24中和性抗体明显促进其细胞活力(P0.05或P0.01),重组CCL2(100 ng/mL)可体外增强HTR-8/SVneo细胞活力(P0.01),但这一作用可被重组IL-24所抑制。结论 CCL2通过抑制IL-24分泌增强人滋养细胞株HTR-8/SVneo细胞的体外活力,从而可能利于囊胚植入和胎盘发育。
[Abstract]:Objective to investigate the effect of interleukin-24 (IL-24) on human trophoblastic activity. Methods the expression of IL-24 and its receptors (IL-20R1, IL-20R2 and IL-22R1) in human chorionic villi during normal early pregnancy was detected by immunohistochemical method. The expression of recombinant thymic stromal lymphopoietin (thymic stromal lymphopoietinine TSLP) and chemokine CCL2 in human choriotrophoblastic cell line HTR-8 / SVneo was detected by In-cell Western method. The effect of IL-24 expression on the activity of HTR-8 / SVneo cells was analyzed by MTT assay. Results IL-24 and its receptors were strongly expressed in trophoblast of normal early pregnancy. Compared with the control group, the expression of IL-24 in HTR-8 / SVneo cells was significantly decreased after stimulation by recombinant CCL2 (P0.001). After treatment with recombinant IL-24, the activity of HTR-8 / SVneo cells decreased significantly (P0.05 or P0.001), whereas the neutralizing antibody of IL-24 significantly increased the viability of HTR-8 / SVneo cells (P0.05 or P0.01). Recombinant CCL2 (100 ng / mL) enhanced the viability of HTR-8 / SVneo cells in vitro (P0.01), but this effect could be inhibited by recombinant IL-24. Conclusion CCL2 can enhance the viability of human trophoblastic cell line HTR-8 / SVneo in vitro by inhibiting IL-24 secretion, which may be beneficial to blastocyst implantation and placental development.
【作者单位】：复旦大学附属妇产科医院研究所生殖免疫教研室;上海交通大学附属第一人民医院妇产科;国家人口计生委计划生育药具重点实验室;
【基金】：国家自然科学基金项目(81571509,31671200) 复旦大学卓学人才计划~~
【分类号】：R714

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