MACC1在宫颈鳞癌组织中的表达及意义

发布时间：2018-06-30 18:44

本文选题：宫颈鳞癌 + MACC1　；参考：《中南大学》2014年硕士论文

【摘要】：目的：探讨结肠癌转移相关基因1(MACC1)蛋白及基因在宫颈鳞癌组织中的表达,及其与宫颈鳞癌临床分期、淋巴结转移及分化程度的关系。方法：采用免疫组织化学法(IHC)和半定量逆转录-聚合酶链反应法(RT-PCR),检测30例宫颈鳞癌组织和15例正常宫颈组织中MACC1蛋白及基因的表达,并对MACC1的表达与宫颈鳞癌临床分期、淋巴结转移及分化程度的关系进行统计学分析。结果：1.免疫组织化学检测结果显示MACC1蛋白主要定位于细胞浆,在宫颈鳞癌组织中的阳性表达率为66.7%,高于正常宫颈组织中的阳性表达率6.67%,两者比较,P0.05,差异具有统计学意义。且与宫颈鳞癌临床分期、淋巴结转移及分化程度相关(P0.05)。2.半定量逆转录-聚合酶链反应法检测结果：MACC1mRNA在宫颈鳞癌组织中的相对表达量为：0.674±0.286,高于正常宫颈组织中的相对表达量0.252±0.034,且与宫颈鳞癌临床分期、淋巴结转移及分化程度相关(P0.05)。结论：MACC1表达上调可能与宫颈鳞癌(I、II期)的发展、淋巴结转移及分化程度相关。
[Abstract]:Objective: to investigate the expression of metastasis associated gene 1 (MACC1) protein and gene in cervical squamous cell carcinoma (SCC), and its relationship with clinical stage, lymph node metastasis and differentiation of cervical squamous cell carcinoma (SCC). Methods: immunohistochemical method (IHC) and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of MACC1 protein and gene in 30 cases of cervical squamous cell carcinoma and 15 cases of normal cervical tissues. The relationship between lymph node metastasis and differentiation was analyzed statistically. The result is 1: 1. The results of immunohistochemistry showed that MACC1 protein was mainly located in cytoplasm, and the positive expression rate of MACC1 protein in cervical squamous cell carcinoma was 66.7%, which was higher than that in normal cervical tissue (6.67%). The difference was statistically significant (P 0.05). And it was related to the clinical stage, lymph node metastasis and differentiation of cervical squamous cell carcinoma (P0.05). The results of semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) showed that the relative expression level of WMACC1 mRNA in cervical squamous cell carcinoma was 0.674 卤0.286, which was higher than that in normal cervical tissue (0.252 卤0.034), and was related to the clinical stage, lymph node metastasis and differentiation of cervical squamous cell carcinoma (P0.05). Conclusion the up-regulation of MCC1 expression may be related to the development of cervical squamous cell carcinoma (stage II), lymph node metastasis and differentiation.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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