不同宫颈病变组织中SCCA、MTA1、P16、Ki-67的表达与高危型HPV感染的相关性研究

发布时间：2018-07-01 18:59

本文选题：宫颈病变 + SCCA　；参考：《华北理工大学》2017年硕士论文

【摘要】：目的探讨不同宫颈病变组织中鳞状细胞癌抗原(SCCA)、肿瘤转移相关因子-1(MTA1)、多肿瘤抑制基因(P16)及细胞核相关抗原(Ki-67)的表达情况,并分析四种因子与高危型HPV感染的相关性。方法收集自2015年3月～2016年11月于华北理工大学附属医院诊治患者123例,其中病例组91例,其中包括宫颈低级别上皮内病变(low-grade squamous intraepithelial lesions,LSIL)31例、宫颈高级别上皮内病变(high-grade squamous intraepithelial lesions,HSIL)31例以及宫颈鳞癌组29例;同时选取慢性宫颈炎组32例作为对照组。所有病理结果均由2位病理科医师证实,且均未采取过放疗、化疗以及激素治疗,收集其临床资料及其经手术或阴道镜活检术取下的宫颈组织,收集上述病人的宫颈分泌物,采用cobas 4800检测技术,检测宫颈分泌物中HPV病毒的DNA,应用免疫组化S-P法,检测不同宫颈组织中SCCA、MTA1、P16及Ki-67的免疫组化染色情况,两种不同宫颈病变组织中的免疫组化结果的比较采用秩和检验;采用Western blot方法检测不同宫颈病变组织中SCCA、MTA1的表达情况,两种不同组织间的Western blot结果的比较用独立样本t检验;并通过斯皮尔曼(Spearman)秩相关检验分别分析四种因子与高危型HPV感染的相关性。结果1免疫组化结果显示,SCCA蛋白在宫颈鳞癌中呈强阳性表达,在HSIL、LSIL中阳性表达,在慢性宫颈炎组表达较弱,两两比较,宫颈鳞癌组织中SCCA的表达水平和染色范围均高于HSIL、LSIL组(P0.05);HSIL、LSIL组的表达高于慢性宫颈炎组(P0.05);HSIL组的表达量和染色强度高于LSIL,但两者差异无统计学意义(P=0.197);Spearman相关分析,γ=0.640(双侧检验P0.05)。Western blot检测结果与免疫组化结果一致,显示随着宫颈病变程度的增加,SCCA蛋白的表达水平和染色范围逐渐增加。2免疫组化结果显示,MTA1蛋白在宫颈鳞癌及HSIL中呈现出阳性或者是强阳性表达,在LSIL、慢性宫颈炎组表达较弱,两两比较,宫颈鳞癌组织中MTA1的表达水平和染色范围高于HSIL、LSIL组,(P0.05);HSIL组均高于LSIL、慢性宫颈炎组(P0.05);LSIL组MTA1的表达高于慢性宫颈炎组,但两者比较差异无统计学意义(P0.05)。Spearman相关分析,γ=0.635(双侧检验P0.05)。Western blot检测结果与免疫组化结果一致,显随着宫颈病变程度的,MTA1蛋白的表达水平和染色范围逐渐增加。3 P16蛋白在宫颈鳞癌中呈强阳性表达,在HSIL、LSIL中阳性表达,在慢性宫颈炎组表达较弱;两两比较,宫颈鳞癌组织中P16的表达水平和染色范围高于HSIL、LSIL组(P0.05);HSIL、LSIL组高于慢性宫颈炎组(P0.05);Spearman相关分析,γ=0.784(双侧检验P0.05)。显示随着宫颈病变程度的,P16蛋白的表达水平和染色范围逐渐增加。4 Ki-67蛋白在宫颈鳞癌中强阳性表达,在HSIL、LSIL中阳性表达,在慢性宫颈炎组表达较弱,两两比较,得出宫颈鳞癌组织中Ki-67的表达高于HSIL、LSIL组(P0.05);HSIL、LSIL组高于慢性宫颈炎组(P0.05)。Spearman相关分析,γ=0.840(双侧检验P0.05);显示随着宫颈病变程度的,Ki-67蛋白的表达水平和染色范围逐渐增加。5 SCCA的表达与HR-HPV的感染尚未发现相关性(P0.05)。6 MTA1、P16、Ki-67的表达与HR-HPV的感染呈正相关(P0.05)。结论1 SCCA蛋白在宫颈鳞癌中的表达程度高于HSIL、LSIL组,HSIL、LSIL组高于慢性宫颈炎。2 MTA1蛋白在宫颈鳞癌中的表达程度高于HSIL组,HSIL组高于LSIL和慢性宫颈炎。3 P16、Ki-67蛋白在宫颈鳞癌中的表达程度明显高于HSIL、LSIL组,HSIL、LSIL组高于慢性宫颈炎组。4宫颈组织中SCCA蛋白的表达程度与HR-HPV的感染尚未发现相关性。5宫颈组织中MTA1、P16、Ki-67蛋白的表达程度与HR-HPV的感染呈正相关。
[Abstract]:Objective to investigate the expression of squamous cell carcinoma antigen (SCCA), tumor metastasis related factor -1 (MTA1), multiple tumor suppressor gene (P16) and nuclear related antigen (Ki-67) in different cervical lesions, and to analyze the correlation between the four factors and high risk HPV infection. 123 cases were treated in the hospital, of which 91 cases were case group, including 31 cases of low-grade squamous intraepithelial lesions, LSIL, 31 cases of high grade cervical intraepithelial lesion (high-grade squamous intraepithelial lesions, HSIL) and 29 cases of cervical squamous cell carcinoma group, and 32 cases of chronic cervicitis group were selected as control. All the pathological results were confirmed by 2 pathologists, and none had been treated with radiotherapy, chemotherapy and hormone therapy. The clinical data and cervical tissue from the operation or colposcopy biopsy were collected, the cervical secretions of the above patients were collected, the Cobas 4800 technique was used to detect the DNA of the HPV virus in the cervical secretions, and the application of the exemptions was not used. Immuno histochemical staining of SCCA, MTA1, P16 and Ki-67 in different cervical tissues was detected by immunohistochemical S-P method. The immunohistochemical results of two different cervical lesions were compared by the rank sum test, and the Western blot method was used to detect the expression of SCCA, MTA1 in different cervical lesions, and the Western blot results between two different tissues. The independent sample t test was used, and the correlation between the four factors and high risk HPV infection was analyzed by the Spielman (Spearman) rank correlation test. Results 1 immunohistochemical results showed that SCCA protein was strongly positive in cervical squamous cell carcinoma, expressed in HSIL, LSIL, weak in chronic cervicitis group, and 22 in cervical squamous cell scale. The expression level and color range of SCCA in cancer tissues were higher than that in HSIL, group LSIL (P0.05), and the expression of HSIL, LSIL group was higher than that of chronic cervicitis group (P0.05); the expression and intensity of HSIL group were higher than LSIL, but there was no significant difference between them (P=0.197); Spearman correlation analysis, gamma =0.640 (bilateral test) detection results and immunity The results of the histochemistry were consistent. With the increase of the degree of cervical lesions, the expression level of SCCA protein and the staining range increased gradually, and the results of.2 immunohistochemical staining showed that MTA1 protein was positive or strongly positive in cervical squamous cell carcinoma and HSIL. In LSIL, the expression of the chronic cervicitis group was weaker. 22, the expression of MTA1 in cervical squamous cell carcinoma tissue was compared. The level and staining range were higher than that of HSIL, group LSIL, (P0.05), group HSIL was higher than LSIL and chronic cervicitis group (P0.05), and the expression of MTA1 in group LSIL was higher than that of chronic cervicitis group, but there was no significant difference between the two groups (P0.05).Spearman related analysis, and the results of gamma =0.635 (bilateral test P0.05) were in accordance with the immunohistochemical results. The degree of cervical lesions, the expression level of MTA1 protein and the range of staining gradually increased the positive expression of.3 P16 protein in cervical squamous cell carcinoma, positive expression in HSIL, LSIL, and weak expression in chronic cervicitis group. 22, the expression level and color range of P16 in cervical squamous cell carcinoma were higher than that of HSIL, LSIL group (P0.05); HSIL, LSIL group was higher than slow. Sexual cervicitis group (P0.05); Spearman correlation analysis, gamma =0.784 (bilateral test P0.05). With the degree of cervical lesions, the expression level of P16 protein and the range of staining gradually increased the strong positive expression of.4 Ki-67 protein in cervical squamous cell carcinoma, positive expression in HSIL, LSIL, in chronic cervicitis group, 22 comparison, to obtain cervical squamous cell carcinoma. The expression of Ki-67 in tissue was higher than that in HSIL, group LSIL (P0.05), and group HSIL and LSIL was higher than that of chronic cervicitis group (P0.05).Spearman related analysis, and gamma =0.840 (bilateral test P0.05). The expression of P16, Ki-67 was positively correlated with HR-HPV infection (P0.05). Conclusion 1 SCCA protein expression in cervical squamous cell carcinoma is higher than HSIL, LSIL group, HSIL, LSIL group is higher than chronic cervicitis,.2 MTA1 protein expression in cervical squamous cell carcinoma is higher than that of HSIL group. The degree of the SCCA protein in the.4 cervical tissues of the chronic cervicitis group was higher than that of the HSIL, group LSIL, HSIL and LSIL. The degree of SCCA protein expression in the cervical tissues of the chronic cervicitis group was not found to be associated with the HR-HPV infection. The expression of P16, Ki-67 protein was positively correlated with the infection of the HR-HPV.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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