上皮性卵巢癌中FAT4和YAP1的表达及意义

发布时间：2018-07-05 07:21

  本文选题：FAT4 + YAP1　； 参考：《安徽医科大学》2017年硕士论文

【摘要】：目的:卵巢上皮性肿瘤是女性生殖系统最常见的卵巢肿瘤,而且卵巢癌一经确诊,超过70%已是中晚期,从而导致了容易复发、预后差以及病死率高等特点,严重威胁了女性的生命和生活质量。因此国内外学者在恶性肿瘤的发生和发展机制中做了非常多的研究,其中上皮组织的Hippo信号通路备受关注,目前多数学者研究证实,恶性肿瘤的发生发展与Hippo信号通路调控出现异常有着密切的关系,主要是促进组织细胞的增殖与抑制细胞的凋亡,加强肿瘤细胞的侵袭和迁移能力,导致肿瘤形成及远处转移。其中FAT4参与Hippo信号通路的调控,YAP1是Hippo信号通路下游的十分重要的分子,参与着恶性肿瘤的发生与发展。本研究采用免疫组织化学方法检测FAT4及Yes-相关蛋白1(YAP1)在上皮性卵巢癌中的表达情况,并对其临床意义及两者在卵巢癌中的相关性进行分析,从而为卵巢癌的研究提供参考和依据。方法1、研究对象:收集我院2014年12月至2015年12月期间初治的卵巢上皮性癌组织标本45例(其中浆液性卵巢癌31例,粘液性卵巢癌14例)。收集同期手术并经病理证实的卵巢上皮性良性肿瘤组织标本25例(其中浆液性囊腺瘤18例,粘液性囊腺瘤7例)依照MDACC组织分级标准进行统计研究。2、标本采集:采集术后卵巢肿瘤组织,大小约1*1*1cm,生理盐水冲洗净后,至-80℃冰箱冷冻保存备用,后进行石蜡包埋等处理。3检测指标及方法:应用免疫组织化学方法检测两组组织标本中FAT4、YAP1的表达情况,分析其与上皮性卵巢癌的临床病理分级及FAT4与YAP1之间的相关性。4数据分析:采用统计软件SPSS16.0对实验结果进行统计学分析,两组间率的比较采用卡方检验,采用Spearman秩相关分析方法,分析FAT4和YAP1之间的相互关系。取?=0.05作为检验水准,P0.05为有统计学差异。结果:(1)FAT4及YAP1在上皮性卵巢癌细胞表达率分别为84.4%和75.5%,而在卵巢上皮良性肿瘤中表达率分别为44%和40%,有统计学差异(P0.05)。本研究发现FAT4和YAP1的表达率在与卵巢癌的分化程度相关(P0.05)(2)应用Spearman对FAT4与YAP1表达的相关性进行相关分析,显示FAT4与YAP1在上皮性卵巢癌表达呈正相关(r=0.565,P0.001)。结论:FAT4及YAP1蛋白在上皮性卵巢癌中的表达明显升高,与卵巢癌的病理分级相关。通过本研究,我们认为FAT4和YAP1可以作为诊断上皮性卵巢癌的生物指标,可作为上皮性卵巢癌组织分级判断的参考指标。可以为卵巢癌的靶向治疗提供一种新思路。
[Abstract]:Objective: ovarian epithelial tumor is the most common ovarian tumor in female reproductive system. A serious threat to women's lives and quality of life. Therefore, scholars at home and abroad have done a lot of research on the pathogenesis of malignant tumor, and the Hippo signaling pathway of epithelial tissue has attracted much attention. The occurrence and development of malignant tumor is closely related to the abnormal regulation of Hippo signaling pathway, which mainly promotes the proliferation of hippo cells, inhibits cell apoptosis, strengthens the ability of invasion and migration of tumor cells, and leads to tumor formation and distant metastasis. FAT4 is involved in the regulation of Hippo signaling pathway and YAP1 is a very important molecule downstream of Hippo signaling pathway, which is involved in the occurrence and development of malignant tumor. In this study, the expression of FAT4 and Yes-associated protein 1 (YAP1) in epithelial ovarian carcinoma was detected by immunohistochemical method, and the clinical significance and the correlation between FAT4 and YAS-associated protein 1 in ovarian cancer were analyzed. So as to provide a reference and basis for the study of ovarian cancer. Methods 1. Participants: 45 cases of epithelial ovarian carcinoma (including 31 cases of serous ovarian cancer and 14 cases of mucinous ovarian cancer) were collected from our hospital from December 2014 to December 2015. A total of 25 cases of ovarian epithelial benign tumors, including 18 cases of serous cystadenoma, were collected. 7 cases of mucinous cystadenoma) were statistically studied according to MDACC tissue grading standard. Specimens were collected and collected. The size of ovarian tumor was about 1 ~ 1 ~ 1 cm after operation. After rinsing with normal saline, cryopreservation was made at -80 鈩，

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