miRNA-143靶向MACC1抑制宫颈癌细胞侵袭

发布时间：2018-07-07 15:30

本文选题：宫颈癌 + miRNA-　；参考：《中国肿瘤临床》2015年18期

【摘要】：目的:探讨mi RNA-143对宫颈癌细胞侵袭能力的影响。方法:采用脂质体转染法瞬时转染mi RNA-143过表达和干扰质粒,Transwell迁移实验检测mi RNA-143过表达和抑制后宫颈癌细胞侵袭能力的改变,生物信息学预测mi RNA-143的作用靶点。mi RNA-143过表达和抑制后Western blot及双荧光素酶报告基因检测其靶点MACC1表达,RT-q PCR检测20例患者宫颈癌和癌旁正常组织标本中mi RNA-143和MACC1 m RNA的表达,分析20例患者宫颈癌组织中mi RNA-143和MACC1 m RNA表达的相关性。结果:Transwell迁移实验显示mi RNA-143过表达的宫颈癌细胞的侵袭能力降低,抑制mi RNA-143后侵袭能力增强。生物信息学预测显示mi RNA-143作用于MACC1的3'-UTR,Western blot及双荧光素酶报告基因结果进一步证实mi RNA-143作用于MACC1的3'-UTR。RT-q PCR显示mi RNA-143过表达的MACC1 m RNA表达下降,而抑制mi RNA-143后MACC1 m RNA表达上升。抑制mi RNA-143表达的宫颈癌细胞中MACC1被干扰后,宫颈癌细胞的侵袭能力显著被抑制。宫颈癌组织中mi RNA-143表达水平显著低于正常宫颈上皮组织,MACC1表达水平显著高于正常宫颈上皮组织,20例患者的宫颈癌组织中mi RNA-143与MACC1 m RNA表达呈负相关。结论:mi RNA-143在宫颈癌中表达水平下降,并可能通过靶向MACC1调节宫颈癌细胞的侵袭能力。
[Abstract]:Objective: to investigate the effect of mi RNA-143 on invasive ability of cervical cancer cells. Methods: the overexpression of mi RNA-143 and the transwell migration of interference plasmid were used to detect the change of invasion ability of cervical cancer cells after the overexpression of mi RNA-143 and inhibition of the invasion ability of cervical cancer cells by liposome transfection. Bioinformatics was used to predict the action target of mi RNA-143. After overexpression and inhibition of mi RNA-143, the expression of mi RNA-143 and MACC1 mRNA was detected by Western blot and double-luciferase reporter gene detection. The expression of mi RNA-143 and MACC1 mRNA was detected in 20 normal tissues of cervical cancer and adjacent to carcinoma by RT-q PCR. The correlation between the expression of mi RNA-143 and MACC1 mRNA in cervical cancer tissues was analyzed. Results the invasive ability of cervical cancer cells with overexpression of mi RNA-143 was decreased, and the invasion ability was increased after inhibition of mi RNA-143. Bioinformatics prediction showed that the expression of mi RNA-143 on MACC1 3- UTRN Western blot and double luciferase reporter gene further confirmed that mi RNA-143 acted on MACC1 3- UTR.RT-q PCR showed that mi RNA-143 overexpression of MACC-1 mRNA decreased, but after inhibiting mi RNA-143, MACC1 mRNA expression increased. The invasive ability of cervical cancer cells was significantly inhibited after the interference of MACC1 in cervical cancer cells which inhibited the expression of mi RNA-143. The expression level of mi RNA-143 in cervical carcinoma was significantly lower than that in normal cervical epithelium (P < 0.05). The expression level of mi RNA-143 was negatively correlated with MACC1 mRNA expression in cervical cancer tissues of 20 patients with normal cervical epithelium. Conclusion the expression level of cell line 1: mi RNA-143 is decreased in cervical cancer, and the invasive ability of cervical cancer cells may be regulated by targeting MACC1.
【作者单位】：南方医科大学基础医学院病理系;
【分类号】：R737.33

【共引文献】