外周血KISS1 mRNA对上皮性卵巢癌的诊断价值

发布时间：2018-07-07 19:20

  本文选题：KISS基因 + CA　； 参考：《中国肿瘤生物治疗杂志》2015年03期

【摘要】：目的:通过与CA125比较,探讨外周血KISS1 mRNA在上皮性卵巢癌(epithelial ovarian cancer,EOC)中的诊断价值。方法:采集2010年1月至2014年1月在南通大学第二附属医院住院拟手术的EOC患者40例手术前一天的外周静脉血和正常健康者静脉血,用RT-PCR法和电化学发光法分别检测外周血KISS1 mRNA和CA125的表达。结果:EOC早期组(Ⅰ~Ⅱ期)、晚期组(Ⅲ~Ⅳ期)KISS1 mRNA的表达量均显著高于正常健康者(均P0.01),而早期与晚期EOC组间比较无显著差异(P0.05);EOC晚期组CA125的表达量明显高于早期组及正常健康者(均P0.01),而早期组与正常健康者比较无显著差异(P0.05)。KISS1 mRNA ROC(receptive operator character curve,ROC)曲线的切值(cutoff)设为0.51、0.72时,阳性预测值(positive predictive value,PPV)分别为0.58和1,阴性预测值(negative predictive value,NPV)分别为0.92和0.7;CA125的cutoff值设为20、100 U/ml时,PPV分别为0.72和1,NPV分别为0.87和0.81。KISS1 mRNA、CA125对EOC均具有中度诊断效能(P=0.34);两者比较,KISS1 mRNA对早期EOC具有较高诊断价值(P=0.018),而CA125对晚期EOC的诊断价值较高(P0.01)。结论:患者外周血KISS1 mRNA与CA125对EOC具有同等的诊断价值,KISS1 mRNA可作为EOC的一个新的肿瘤标志物;两者的联合检测可提高EOC的诊断效能,特别是早期EOC的诊断率。
[Abstract]:Objective: to investigate the diagnostic value of KISS1 mRNA in epithelial ovarian cancer (epithelial ovarian cancerEOC) by comparing with CA125. Methods: from January 2010 to January 2014, the peripheral venous blood of 40 EOC patients who were hospitalized in the second affiliated Hospital of Nantong University and the venous blood of healthy people were collected one day before the operation. The expression of KISS1 mRNA and CA125 in peripheral blood were detected by RT-PCR and electrochemiluminescence respectively. Results the expression of KISS1 mRNA in early stage (鈪，

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