GPC3在卵巢透明细胞腺癌中的表达及临床意义

发布时间：2018-07-08 21:32

  本文选题：卵巢透明细胞腺癌 + 磷脂酰肌醇蛋白聚糖　； 参考：《南京医科大学学报(自然科学版)》2015年05期

【摘要】：目的 :探讨磷脂酰肌醇蛋白聚糖3(glypican-3,GPC3)在卵巢透明细胞腺癌中的表达及临床意义。方法 :采用免疫组织化学方法分别检测83例卵巢癌组织(透明细胞腺癌45例、浆液性癌20例、黏液性癌18例)、20例浆液性囊腺瘤及20例正常卵巢组织中GPC3蛋白的表达情况,实时荧光定量PCR法检测38例卵巢癌组织(透明细胞腺癌20例、浆液性癌10例、黏液性癌8例)、5例浆液性囊腺瘤和5例正常卵巢组织中m RNA的表达情况,并分析GPC3与卵巢透明细胞腺癌临床病理学参数及预后的相关性。结果:1卵巢透明细胞腺癌中GPC3蛋白阳性表达率和m RNA表达量(82.2%;1.326 1±0.493 6)均明显高于浆液性癌(20%;0.426 5±0.029 4)、黏液性癌(5%;0.265 2±0.103 9)、浆液性囊腺瘤(0%;0.141 3±0.011 3)、正常卵巢组织(0%;0.291 4±0.048 1),差异有统计学意义(P均0.01);2GPC3蛋白阳性表达率及GPC3 m RNA表达量与卵巢透明细胞腺癌的临床分期有相关性,Ⅲ~Ⅳ期患者中的表达(100%;1.808 1±0.265 7)显著高于Ⅰ~Ⅱ期患者(71.4%;1.045 8±0.403 6)(P值0.01);3卵巢透明细胞腺癌铂类药物耐药患者GPC3蛋白阳性表达率及m RNA表达量(100%;1.808 1±0.265 7)均高于铂类药物敏感型患者(70.3%;0.991 8±0.330 3)(P0.05);4GPC3蛋白阳性表达率与卵巢透明细胞腺癌患者的预后相关,预后差的患者中阳性率及表达量高,差异有统计学意义(P0.05),Kaplan-Meier单因素生存分析亦显示GPC3蛋白为影响预后的因素(P=0.048);5卵巢透明细胞腺癌中GPC3蛋白阳性表达率和m RNA表达量与患者的淋巴结转移和远处器官转移无相关性(P0.05)。结论 :GPC3在病理鉴别诊断中有重要意义,GPC3与卵巢透明细胞腺癌患者的临床分期和铂类耐药相关,提示其可能在卵巢透明细胞腺癌的发生发展起重要作用,有望成为卵巢透明细胞腺癌的预后指标和潜在治疗靶标。
[Abstract]:Objective: to investigate the expression and clinical significance of phosphatidylinositol proteoglycan 3 (glypican-3) in ovarian clear cell adenocarcinoma. Methods: the expression of GPC3 protein in 83 cases of ovarian carcinoma (45 cases of clear cell adenocarcinoma, 20 cases of serous carcinoma, 18 cases of mucinous carcinoma) and 20 cases of serous cystadenoma and 20 cases of normal ovary were detected by immunohistochemical method. The expression of mRNA in 38 cases of ovarian carcinoma (20 cases of clear cell adenocarcinoma, 10 cases of serous carcinoma, 8 cases of mucinous carcinoma) and 5 cases of serous cystadenoma and 5 cases of normal ovary were detected by real-time fluorescence quantitative PCR. The relationship between GPC3 and clinicopathological parameters and prognosis of ovarian clear cell adenocarcinoma was analyzed. Results the positive expression rate and mRNA expression of GPC3 protein in ovarian clear cell adenocarcinoma were significantly higher than those in serous carcinoma (0.493 卤0.029 4), mucinous carcinoma (0.265 2 卤0.103 9), serous cystadenoma (0.0.141 3 卤0.011 3) and normal ovary (0.291 4 卤0.048 1). The positive expression rate of GPC3 protein and the expression of GPC3 mRNA were correlated with the clinical stage of ovarian clear cell adenocarcinoma. The expression of GPC3 protein and mRNA in patients with stage 鈪，

本文编号：2108801