玻璃化冻融囊胚助孕妊娠妇女娩出胎盘Grb10表达分析研究

发布时间：2018-07-10 05:56

本文选题：囊胚 + 玻璃化冷冻　；参考：《重庆医学》2017年11期

【摘要】：目的对玻璃化冻融囊胚助孕妊娠妇女娩出胎盘中生长因子受体结合蛋白10(Grb10)表达进行分析,评价其安全性。方法收集2012年1月至2014年5月在福建省妇幼保健院产科门诊玻璃化冻融囊胚助孕妊娠的妇女(观察组)50例及同期自然妊娠妇女(对照组)50例病例数据和胎盘标本。采用免疫组织化学、Western blot检测胎盘Grb10蛋白的表达,Real-time PCR检测Grb10mRNA表达。对两组妊娠妇女的产科结局及胎盘Grb10的表达进行比较分析。结果两组孕龄、胎龄,胎儿性别、体质量、身长、头围、腹围比较差异均无统计学意义(P0.05),娩出胎盘面积、胎盘质量比较差异有统计学意义(P0.05)。两组胎盘组织Grb10蛋白表达率、表达强度、免疫组织化学评分比较差异均无统计学意义(P0.05)。Real-time PCR、Western blot分析结果进一步显示两组胎盘组织中Grb10mRNA与蛋白表达差异均无统计学意义(P0.05)。结论玻璃化冻融囊胚助孕有可能会增加娩出胎盘的面积与质量,但发育相关分子Grb10在胎盘的表达未见明显改变。
[Abstract]:Objective to analyze the expression of growth factor receptor binding protein 10 (Grb10) in placenta of pregnant women assisted by vitrified freeze-thawed blastocysts and evaluate its safety. Methods from January 2012 to May 2014, 50 pregnant women with vitrified freeze-thawed blastocysts (observation group) and 50 women with natural pregnancy (control group) in Fujian Maternal and Child Health Hospital were collected. The expression of Grb10 protein in placenta was detected by Western blot and Real-time PCR was used to detect the expression of Grb10 mRNA. The obstetrical outcome and expression of Grb10 in placenta of two groups of pregnant women were compared and analyzed. Results there were no significant differences in gestational age, fetal sex, body weight, body length, head circumference and abdominal circumference between the two groups (P0.05), but there were significant differences in placental area and placental mass between the two groups (P0.05). There was no significant difference in the expression of Grb10 protein, expression intensity and immunohistochemical score between the two groups (P0.05). The results of Western blot analysis showed that there was no significant difference in the expression of Grb10 mRNA and protein between the two groups (P0.05). Conclusion vitrified freeze-thawed blastocysts may increase the area and quality of placenta delivery, but the expression of developmental related molecule Grb10 in placenta has not changed significantly.
【作者单位】：福建医科大学附属福建省妇幼保健院辅助生殖技术研究室;南方医科大学南方医院妇产科生殖医学中心;
【基金】：福建省自然科学基金(2014J01277) 福建省卫生系统中青年骨干人才培养项目(2013-ZQN-JC-7) 福建省妇幼保健院科研基金(闽妇幼13-08,13-09,10-01)
【分类号】：R714.8

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