基因芯片筛选新疆维吾尔妇女宫颈癌差异表达基因

发布时间：2018-07-12 21:37

本文选题：基因芯片 + 宫颈癌　；参考：《中国生物化学与分子生物学报》2015年06期

【摘要】：新疆南部维吾尔族聚居区是宫颈癌高发区.本文旨在利用基因芯片技术筛选与维吾尔族妇女宫颈癌发生相关的基因.首先,分别提取5例新疆维吾尔妇女宫颈癌和5例子宫肌瘤组织(对照)的m RNA,逆转录成c DNA,并用Cy3-d UTP标记子宫肌瘤组织的cDNA,用Cy5-d UTP标记宫颈癌组织的c DNA,制成芯片杂交探针.为筛选出宫颈癌组织中差异表达的基因,上述标记探针分别与含有20 000条人类基因的Affymetrix基因芯片进行杂交,杂交信号用Gene Chip Scanner 3000扫描仪扫描,并用芯片图像分析软件(SAM software)分析扫描结果.筛选出的差异表达基因经GO(Gene Ontology)分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)信号通路分析,确定其在宫颈癌中的作用.基因芯片筛选结果显示,在宫颈癌组织中发现2 758个差异表达基因,其中1 326个上调基因,1 432个下调基因.GO分析和KEGG信号通路分析表明,表达差异在两倍以上的基因涉及168个信号通路,包括细胞粘附分子、细胞周期以及MAPK和mTOR信号通路等.上述结果表明,基因芯片技术筛选出大量与宫颈癌发生相关的基因,其中表达差异显著的基因涉及细胞粘附分子、细胞周期和m TOR等信号通路.
[Abstract]:The Uygur area in southern Xinjiang has a high incidence of cervical cancer. The purpose of this study was to screen the genes associated with cervical cancer in Uygur women by gene chip technique. Firstly, the mRNAs of 5 cases of cervical cancer in Xinjiang Uygur women and 5 cases of uterine leiomyoma tissue (control group) were extracted, and then the cDNA of uterine leiomyoma tissue was labeled with Cy3-d UTP. The cDNA of cervical cancer tissue was labeled with Cy5-d UTP, and the microarray hybridization probe was made. In order to screen differentially expressed genes in cervical cancer tissues, the above labeled probes were hybridized with Affymetrix gene chip containing 20 000 human genes. The hybridization signals were scanned by Gene Chip Scanner 3000 scanner. The scan results were analyzed by chip image analysis software (Sam software). The differentially expressed genes were analyzed by go (Gene Ontology) and KEGG (Kyoto Encyclopedia of genes and genomes) to determine their role in cervical cancer. The results of gene chip screening showed that 2 758 differentially expressed genes were found in cervical cancer tissues, among which 1 326 up-regulated genes were identified by .go analysis and KEGG signal pathway analysis. The differentially expressed genes involved 168 signaling pathways, including cell adhesion molecules, cell cycle, MAPK and mTOR signaling pathways. These results suggest that a large number of genes related to cervical carcinogenesis were screened by gene chip technique, among which the differentially expressed genes were involved in cell adhesion molecules, cell cycle and m TOR signaling pathways.
【作者单位】：新疆大学生命科学与技术学院;新疆维吾尔自治区肿瘤医院;新疆维吾尔自治区人民医院;
【基金】：国家自然科学基金(No.31060025) 新疆生物资源基因工程重点实验室开放课题(No.XJDX0201-2013-01)~~
【分类号】：R737.33

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