PDCD5与XIAP蛋白在子宫腺肌病中的表达及意义

发布时间：2018-07-13 19:42

【摘要】：目的：探讨凋亡及其相关因子程序性细胞死亡分子5(programmed cell death5, PDCD5)与X-连锁调亡抑制蛋白(X-linkedinhibitor of apoptosis protein, XIAP)在子宫腺肌病(adenomyosis, AM)发病中的作用，为AM的临床诊治及深入研究提供实验依据。方法：收集子宫全切术患者子宫标本，筛选出其中经病理确诊为AM的标本共30例，取其异位区内膜组织（异位组）和非异位区内膜组织（在位组）；同期收集并筛选出其中经病理确诊仅为子宫肌瘤的子宫标本共30例，取其非肌瘤区内膜组织作为对照组。所有入选实验对象在术前半年内均未服用激素类和抗子宫腺肌病药物。采用原位末端标记（TdT-mediated dUTP Nick-End Labeling, Tunel）细胞凋亡检测试剂盒检测三组内膜腺上皮细胞凋亡情况，计数各组内膜腺上皮细胞凋亡指数(apoptosis index,AI)。免疫组化法检测PDCD5与XIAP在三组内膜组织中的表达，应用Image Pro6.0专业图像分析系统分析PDCD5与XIAP阳性细胞平均光密度值（mean optical density, MOD），分析二者在三组内膜腺上皮细胞中的表达差异及相关性。实验数据采用SPSS17.0软件进行统计学分析，P0.05为差异有统计学意义。结果：1、异位组内膜细胞凋亡指数AI略小于在位组，差异无统计学意义（P＞0.05），明显小于对照组且差异有统计学意义(P0.05)；在位组内膜细胞AI明显小于对照组，差异有统计学意义(P0.05)。2、PDCD5在三组内膜中的表达，异位组明显低于对照组，差异有统计学意义(P0.05)；在位组低于对照组，差异有统计学意义（P0.05）；在位组略低于异位组，差异无统计学意义（P＞0.05）。3、XIAP在三组内膜中的表达，异位组明显高于对照组，差异有统计学意义(P0.05)；在位组高于对照组，差异有统计学意义（P0.05）在位组略低于异位组，，差异无统计学意义（P＞0.05）4、相关性：PDCD5与XIAP在异位组呈负相关（r=－0.415, P=0.0230.05），在在位组呈负相关（r=-0.382, P=0.0370.05），在对照组呈不相关(r=－0.126,P=0.5060.05)。结论：1、与对照组相比，异位组和在位组内膜细胞凋亡指数AI都明显减小，说明在AM的形成过程中可能确实存在内膜细胞的凋亡异常，这种异常可能是直接或间接参与形成AM的重要因素之一。2、与对照组相比，异位组和在位组中促凋亡蛋白PDCD5与凋亡抑制蛋白XIAP的表达都存在明显差异，PDCD5表达的明显下调及XIAP表达的明显上调，提示PDCD5和XIAP的表达异常可能是促成AM患者子宫内膜细胞出现凋亡异常现象的重要原因之一，是参与AM发病并最终促使AM形成的重要原因之一。3、PDCD5与XIAP在异位和在位内膜组织中的表达均呈负相关，提示二者存在明显的相互拮抗或负反馈调节关系。此外，三组内膜组织中，异位组中PDCD5的表达最低，XIAP的表达最高，这进一步表明PDCD5与XIAP的这种相互拮抗或负反馈调节作用在异位内膜组织中表现得更为明显，提示二者在内膜细胞中表达异常导致的相互作用失衡可能是异位内膜细胞能适应异位区环境并在其中生长，从而进一步促进AM发生发展的关键因素之一。
[Abstract]:Objective: To explore the role of apoptosis and its related factors, programmed cell death molecule 5 (programmed cell death5, PDCD5) and X- linked fall suppression protein (X-linkedinhibitor of apoptosis protein, XIAP) in the pathogenesis of adenomyosis (adenomyosis, AM), and provide experimental basis for clinical diagnosis and treatment and in-depth study. A total of 30 cases of uterine specimens with hysterectomy were selected and 30 specimens were confirmed by pathology. The endometrium endometrium (ectopic tissue) and non ectopic endometrium tissue (incumbent group) were selected. 30 cases of uterine specimens, which were pathologically diagnosed as myoma of uterus, were collected and screened in the same period. The non myoma endometrium tissue was taken as the control group. The three groups of endometrial gland epithelial cells apoptosis were detected by the TdT-mediated dUTP Nick-End Labeling (Tunel) cell apoptosis detection kit, and the apoptosis index (apoptosis index, AI) of the endometrium epithelial cells (apoptosis index, AI) was counted. The expression of PDCD5 and XIAP in the three groups of endometrium was detected by the immunohistochemical method. The average optical density value of PDCD5 and XIAP positive cells (mean optical density, MOD) was analyzed by the Image Pro6.0 professional image analysis system. The difference and correlation between the two groups in the three groups of endometrial gland epithelial cells were analyzed. The experimental data were carried out by SPSS17.0 software. The results of P0.05 were statistically significant. Results: 1, the apoptotic index AI of endometrium cells in ectopic group was slightly smaller than that of the incumbent group (P > 0.05), which was significantly smaller than that in the control group (P0.05), and the AI in the eutopic endometrium was significantly smaller than that in the control group (P0.05).2, PDCD5 in three groups. The expression of the membrane in the ectopic group was significantly lower than that in the control group (P0.05), and the difference was statistically significant (P0.05) in the incumbent group. The difference was slightly lower than the ectopic group (P > 0.05).3, and XIAP was in the three groups of endometrium, and the ectopic group was significantly higher than the control group, the difference was statistically significant (P0.05). The difference was significantly higher than that of the control group (P0.05), the difference was slightly lower than that of the ectopic group (P > 0.05) 4, and the correlation was negative correlation between PDCD5 and XIAP (r= 0.415, P=0.0230.05) in the ectopic group (r=-0.382, P=0.0370.05) and in the control group (r = - 0. 12 6, P=0.5060.05). 1, 1, compared with the control group, the apoptotic index of the endometrium cells in the ectopic and the incumbent groups decreased obviously, indicating that the apoptosis of the endometrium may be abnormal in the formation of AM, which may be one of the important factors to directly or indirectly participate in the formation of AM,.2, which is compared with those in the group, and the apoptotic protein PD in the ectopic group and the incumbent group. There are obvious differences in the expression of CD5 and apoptosis inhibitory protein XIAP, the obvious downregulation of PDCD5 expression and the obvious up-regulation of the expression of XIAP, suggesting that the abnormal expression of PDCD5 and XIAP may be one of the important reasons for the abnormal phenomenon of apoptosis in endometrium cells of AM patients. It is one of the important reasons for participating in AM hair disease and ultimately contributing to the formation of AM.3, P. The expression of DCD5 and XIAP in ectopic and eutopic endometrium was negatively correlated, suggesting that there were obvious antagonism or negative feedback regulation between the two groups. In addition, the expression of PDCD5 was the lowest in the three group of endometrium tissues, and the expression of XIAP was the highest. This further indicated that the mutual antagonism or negative feedback regulation of PDCD5 and XIAP was ectopic and negative feedback. The expression of endometrium in endometrium is more obvious, suggesting that the imbalance in the expression of two of the endometrium cells may be one of the key factors that can further promote the development of AM by the ectopic endometrium cells that can adapt to the ectopic environment and grow in it.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

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