PSD-95参与大鼠孕期铅暴露对子代海马突触影响的实验研究

发布时间：2018-07-20 14:21

【摘要】：目的:研究大鼠孕期铅暴露后子代海马突触变化与PSD-95表达改变的相关性,探讨铅暴露损伤学习记忆功能的机制。方法:通过饮用0.02%醋酸铅水溶液建立孕期铅暴露模型,利用原子吸收分光光度仪检测血铅,透射电镜技术检测海马突触密度,蛋白印记技术检测子代大鼠海马组织中VGLUT、VGAT和PSD-95的表达。结果:对照组和铅暴露组雄性21 d大鼠体重分别为(55.73±4.23)g和(56.01±5.97)g,无显著差异(P0.05);对照组与铅暴露组雄性21 d大鼠血铅分别为(10.2±2.1)μg/L和(301.2±34.8)μg/L,血铅水平明显升高(P0.01);对照组与铅暴露组雄性21 d大鼠单位面积突触数目分别为32.79±2.03和23.46±1.97,突触密度明显减少(P0.01);铅暴露后,VGAT的表达量没有明显变化(P0.05),VGLUT和PSD-95的表达量明显减少(P0.01)。结论:PSD-95表达减少引起海马兴奋性突触数目的下降是发育期铅暴露损伤学习记忆功能的可能机制。
[Abstract]:Aim: to study the relationship between the changes of hippocampal synapses and PSD-95 expression in offspring after lead exposure during pregnancy and to explore the mechanism of damage to learning and memory function by lead exposure. Methods: lead exposure model was established by drinking 0.02% lead acetate solution during pregnancy. Blood lead was detected by atomic absorption spectrophotometer and synaptic density of hippocampus was measured by transmission electron microscopy. Protein imprinting technique was used to detect the expression of VGLUTT VGAT and PSD- 95 in rat hippocampus. Results: the body weight of male rats in the control group and lead exposure group were (55.73 卤4.23) g and (56.01 卤5.97) g, respectively (P0.05), and the blood lead levels in the control group and lead exposure group were (10.2 卤2.1) 渭 g / L and (301.2 卤34.8) 渭 g / L, respectively (P0.01), while those in the control group and lead exposure group were significantly increased (P0.01). The number of synapses per unit area of male rats was 32.79 卤2.03 and 23.46 卤1.97, respectively, and the synaptic density decreased significantly (P0.01), while the expression of VGAT and PSD-95 decreased significantly after lead exposure (P0.05). Conclusion the decrease of the number of excitatory synapses induced by the decrease of the expression of PSD-95 in the hippocampus is the possible mechanism of the impairment of learning and memory function by lead exposure during development.
【作者单位】：第四军医大学军事预防医学院军队劳动与环境卫生学教研室;
【基金】：国家重点基础研究发展计划(973计划,2012CD525002) 国家自然科学基金(81273101)
【分类号】：R714.2

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