阴道分泌物念珠菌感染检测方法对比分析

发布时间：2018-07-25 10:57

【摘要】：目的分析常规镜检、荧光PCR核酸扩增法和真菌显色培养法在阴道分泌物真菌检测结果的相关性。方法选取2014-2016年于该院就诊的疑似阴道炎患者,收集镜检阴道分泌物真菌阳性和阴性标本各500例,用荧光PCR核酸扩增法鉴定念珠菌类型,并将100份荧光PCR核酸扩增法检测结果阳性标本进行真菌微生物培养,验证分型结果的正确率。结果荧光PCR核酸扩增法和常规镜检一致性检验Kappa值为0.632,二者一致性差。500例患者阴道分泌物常规镜检阳性标本,荧光PCR核酸扩增法测得白色念珠菌感染382例(76.4%),光滑假丝酵母菌感染73例(14.6%),热带假丝酵母菌感染10例(2.0%),白色念珠菌合并光滑假丝酵母菌感染3例(0.6%),其他真菌感染32例(6.4%)。500例患者阴道分泌物常规镜检阴性标本,荧光PCR核酸扩增法鉴定阳性共152例,其中白色念珠菌130例,光滑假丝酵母菌16例,热带假丝酵母菌6例。荧光PCR核酸扩增法与CHROMAgar快速显色法检测结果比较,差异无统计学意义(χ2=0.131,P=0.936)。结论对于有临床表现而镜检阴性的患者,建议行荧光PCR核酸扩增快速分型鉴定或真菌培养鉴定。
[Abstract]:Objective to analyze the correlation between routine microscopic examination, fluorescent PCR nucleic acid amplification and fungal coloration in vaginal secretions. Methods A total of 500 positive and 500 negative specimens of vaginal secretions were collected from suspected vaginitis patients from 2014 to 2016. The type of Candida was identified by fluorescence PCR nucleic acid amplification. 100 positive samples of fluorescent PCR nucleic acid amplification were cultured to verify the accuracy of the typing results. Results the Kappa value of fluorescence PCR nucleic acid amplification method and routine microscopic examination was 0.632. 382 cases (76.4%) of Candida albicans infection, 73 cases (14.6%) of Candida smoothing infection, 10 cases (2.0%) of Candida tropicalis infection, 3 cases (0.6%) of Candida albicans with Candida smoothing infection and 32 cases of other fungal infections were detected by fluorescence PCR nucleic acid amplification method. Cases (6.4%). 500 cases of vaginal secretions were negative for routine microscopic examination. A total of 152 cases were identified by fluorescence PCR nucleic acid amplification, including 130 cases of Candida albicans, 16 cases of Candida smooth and 6 cases of Candida tropicalis. There was no significant difference between fluorescent PCR nucleic acid amplification method and CHROMAgar rapid chromogenic assay (蠂 ~ 2 ~ (2) 0.131 ~ (-1) P ~ (0. 936). Conclusion for the patients with clinical manifestations and negative microscopic examination, it is recommended to carry out rapid typing or fungal culture identification by fluorescence PCR nucleic acid amplification.
【作者单位】：四川省人民医院检验科;
【基金】：国家自然科学基金资助项目(81271047) 四川省科技厅科研基金(2016JY0082) 四川省卫生和计划生育委员会科研基金(17PJ504)
【分类号】：R440;R711.3

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