子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义
发布时间：2018-07-26 14:44
【摘要】：子痫前期(Preeclampsia,PE)是妊娠期常见内科并发症——妊娠期高血压疾病中的一种类型,在产科较为常见,其诊断要点是孕妇于妊娠20周后首次出现收缩压≥140mm Hg和(或)舒张压≥90mm Hg,加上以下新发条件之一:蛋白尿(≥300mg/24h,或随机尿蛋白+),母体器官功能障碍(包括肾功能不全、肝脏损害、神经或血液系统并发症)及子宫胎盘功能不全(可能导致胎儿生长受限)。从全球来看,在低收入和中等收入的国家,每年有超过100万妇女死于妊娠相关疾病,大约10%的女性在妊娠期间患有高血压,其中并发子痫前期的占2%~8%。考虑到胎盘介于母体和胎儿之间,是维持胎儿宫内生长发育的重要器官,基于胎盘从母体娩出后PE临床症状能够较快缓解或可以自愈的事实,推测子痫前期的发病与胎盘的病理生理变化密切相关。大多学者认为胎盘的结构较为复杂,滋养细胞是其主要的细胞类型,而滋养细胞功能异常会导致对孕妇血管的侵袭障碍,即造成子宫螺旋动脉重塑不足,导致子宫胎盘血流灌注减少、胎盘缺血缺氧,进而释放一些细胞因子进入母体循环,损害孕妇血管内皮细胞。相关的发病学说还涉及遗传、营养、炎症及胰岛素抵抗等方面,但截止到目前,子痫前期的具体发病机制仍不清楚。MicroRNA(miRNA)是一类长度为19~25个核苷酸非编码蛋白质的单链RNA,在基因转录后水平抑制信使RNA(m RNA)的表达或致其降解。自从研究者成功识别了3707个成熟的miRNAs之后,miRNA数据库得以建立,生物信息学分析表明人类超过30%的基因可能受miRNAs的调控,最近,越来越多的证据表明胎盘组织中异常表达的miRNAs与子痫前期的发病有关。趋化因子受体4(CXCR4)是趋化因子基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1,又称CXCL12)的特异性受体,两者有较强的亲和力,通常形成SDF-1/CXCR4生物轴发挥生理学效应。有研究证实,SDF-1/CXCR4轴不仅影响免疫细胞的活化、迁移和募集,还参与血管生成、造血功能、胚胎发育以及肿瘤的侵袭和转移等多种进程。另外有学者在癌症相关的研究中发现CXCR4可能是miR-155的一个靶基因,miR-155通过调控CXCR4基因的表达,影响癌症的血管生成及癌细胞的功能,进而参与癌症的发生。但在子痫前期发病过程中,miR-155与CXCR4之间的表达及如何参与子痫前期的发病机制尚未见相关的研究报道。目的本研究旨在利用实时荧光定量PCR(Real-time PCR)的实验方法,检测重度子痫前期组和正常对照组孕妇胎盘组织中miR-155和CXCR4 m RNA的表达水平,采用链霉亲和素—生物素—过氧化物酶复合物(即SABC法)免疫组织化学的实验方法、使用高通量检测工具——组织芯片对子痫前期组和正常对照组孕妇胎盘组织中CXCR4蛋白的定位及表达进行分析,初步探讨胎盘组织中miR-155和CXCR4的表达以及与子痫前期发病的关系。资料与方法1一般资料1.1 Real-time PCR研究对象选取2015年11月至2016年02月在河南省妇幼保健院产科住院,临床确诊为重度子痫前期、最终以剖宫产术分娩的30例孕妇的胎盘组织作为实验组(s PE组),同期因社会因素行剖宫产术分娩的30例健康孕妇的胎盘组织作为正常对照组(N组)用于检测m RNA的表达。1.2胎盘组织芯片研究对象同一课题组前期收集2007年12月12日至2010年12月31日在郑州大学第三附属医院住院,临床诊断为子痫前期、最终以剖宫产术分娩的80例孕妇的胎盘组织,同期因社会因素行剖宫产术分娩的58例健康孕妇的胎盘组织,由河南省妇幼健康转化医学工程实验室用于构建一种高通量检测工具——胎盘组织芯片,用来分析CXCR4蛋白的定位及表达。1.3研究对象诊断及纳入标准子痫前期及其不同分类的诊断标准严格按照人民卫生出版社出版的第八版《妇产科学》的诊断标准,整个孕期血压低于140/90 mm Hg,无尿蛋白出现,无母体内、外科及自身免疫性疾病等合并症,无其他产科并发症及胎儿异常情况等将纳入正常对照组。本实验中所纳入的全部研究对象均为单胎妊娠、剖宫产分娩。2方法采用实时荧光定量PCR技术从m RNA水平检测实验组和正常对照组孕妇胎盘组织中miR-155及CXCR4的表达情况;采用Spearman方法分析实验组孕妇胎盘组织中miR-155和CXCR4 m RNA表达的相关性;采用链霉亲和素—生物素—过氧化物酶复合物(即SABC法)免疫组织化学的实验方法从蛋白水平检测河南省妇幼健康转化医学工程实验室前期成功构建的胎盘绒毛滋养细胞(VCT)组织芯片(正常对照组42例,子痫前期56例)和绒毛外滋养细胞(EVCT)组织芯片(正常对照组29例,子痫前期47例)中CXCR4蛋白的表达及定位。3统计学方法实验过程中所得数据均采用SPSS 22.0统计软件进行分析。计量资料表示为均数±标准差((?)±s),使用2-△△Ct方法分析Real time-PCR实验结果。检验数据正态分布及方差齐性后,两者均符合者使用两独立样本t检验。相关分析采用Spearman相关分析法。免疫组化的实验结果为有序多分类的等级资料,使用秩和检验进行统计,以α=0.05为检验水准。结果1实验组和正常对照组孕妇临床特征的比较实验组(s PE组)孕妇年龄在22~35岁之间,平均为27.7±2.8岁,正常对照组(N组)孕妇年龄在20~32岁之间,平均为28.8±2.5岁。s PE组的分娩时体重指数(Body Msaa Index,BMI)、分娩孕周、收缩压、舒张压、平均动脉压、新生儿出生体重、尿蛋白分别为21.2±2.9 kg/m2、37.2±2.7周、169.5±17.6 mm Hg、112.4±15.7mm Hg、131.5±15.6 mm Hg、2210.6±905.1g、5.6±2.6 g/L。而正常对照组分别为22.2±2.4 kg/m2、38.7±0.8周、112.6±9.4 mm Hg、71.8±7.1mm Hg、85.6±7.5 mm Hg、3650.5±403.6g、0 g/L。在孕妇年龄、分娩时BMI及分娩孕周方面,两组相比差异无统计学意义(P0.05);在收缩压、舒张压、平均动脉压及尿蛋白方面,实验组均显著高于正常对照组,在新生儿出生体重方面,实验组低于正常对照组,且差异均有统计学意义(P0.05)。2 miR-155和CXCR4 m RNA在实验组和正常对照组孕妇胎盘组织中的表达miR-155、CXCR4 m RNA在实验组和正常对照组孕妇胎盘组织中的相对表达量依次分别为1.53±0.92、0.87±0.73;0.54±0.38、1.53±0.73,两组比较差异具有统计学意义(P0.05)。3 miR-155和CXCR4 m RNA在实验组孕妇胎盘组织中表达的相关性miR-155在实验组孕妇胎盘组织中的相对表达量为1.53±0.92,CXCR4m RNA在实验组孕妇胎盘组织中的相对表达量为0.54±0.38,采用Spearman检验对实验组孕妇胎盘组织中miR-155及CXCR4 m RNA进行相关性分析,结果显示:二者呈显著负相关关系(r=㧟0.773,P0.05)。4 CXCR4蛋白在胎盘VCT和EVCT组织芯片中的表达及定位CXCR4蛋白在胎盘滋养细胞和绒毛外滋养细胞的细胞膜和细胞质中均可见表达。通过对所有位点染色结果进行分析显示:胎盘VCT组织芯片中,CXCR4蛋白在子痫前期组的阳性表达率为48.21%(27/56),正常对照组的阳性表达率为83.33%(35/42),两组相比差异有统计学意义(U=715.5,P0.05);胎盘EVCT组织芯片中:CXCR4蛋白在子痫前期组的阳性表达率为48.94%(23/47),正常对照组为79.31%(23/29),两组相比差异有统计学意义(U=362.0,P0.05)。结论重度子痫前期患者胎盘组织中miR-155可能通过调控CXCR4的表达参与子痫前期的发病。
[Abstract]:Preeclampsia (Preeclampsia, PE) is one of the common internal complications of pregnancy, which is one of the types of hypertensive disorders in pregnancy. It is more common in obstetrics. The main point of diagnosis is that after 20 weeks of pregnancy, the first occurrence of systolic pressure of more than 140mm Hg and (or) diastolic pressure is more than 90mm Hg, plus one of the following new conditions: proteinuria (> 300mg/24h, or random). Urinary protein +), maternal organ dysfunction (including renal dysfunction, liver damage, neurological or blood system complications) and uterine placenta dysfunction (which may lead to fetal growth restriction). Globally, more than 1 million women die of pregnancy related diseases in low and middle income countries, and about 10% of women are in pregnancy. Between the preeclampsia and the preeclampsia, 2%~8%. takes into account that the placenta is between the mother and the fetus, and is an important organ to maintain the fetal intrauterine growth and development, based on the fact that the PE clinical symptoms can be quickly relieved or self healing after the delivery of the mother body from the mother body. It is speculated that the pathogenesis of preeclampsia is closely related to the pathophysiological changes of the placenta. Most scholars believe that the placental structure is more complex, trophoblast is the main cell type, and the dysfunction of trophoblast will lead to the invasion of blood vessels in pregnant women, that is, the insufficiency of the uterine spiral artery, the decrease of the uterine placenta blood flow, the ischemia and hypoxia of the placenta, and the release of some cytokines into the mother body. Circulation, which damages the vascular endothelial cells of pregnant women. The related pathogenesis also involves heredity, nutrition, inflammation and insulin resistance. But at present, the specific pathogenesis of preeclampsia remains unclear..MicroRNA (miRNA) is a single strand RNA of a class of 19~25 nucleotide non coding proteins, which inhibits messenger R at the post transcriptional level. The expression or degradation of NA (m RNA). Since the researchers successfully identified 3707 mature miRNAs, the miRNA database has been established. Bioinformatics analysis shows that more than 30% of the human gene may be regulated by miRNAs. Recently, more and more evidence suggests that the abnormal expression of miRNAs in the placenta is associated with the onset of preeclampsia. Chemokine receptor 4 (CXCR4) is a specific receptor for chemokine matrix cell derived factor -1 (stromal cell-derived factor-1, SDF-1, and CXCL12). Both have strong affinity and usually form a SDF-1/CXCR4 biological axis to play physiological effects. Also involved in a variety of processes, such as angiogenesis, hematopoiesis, embryonic development, and tumor invasion and metastasis. Other scholars have found that CXCR4 may be a target gene for miR-155 in cancer related studies. MiR-155 affects the angiogenesis of cancer and the function of cancer cells by regulating the expression of CXCR4 gene, and then participates in the occurrence of cancer. However, the expression of miR-155 and CXCR4 and how to participate in the pathogenesis of preeclampsia have not been reported in the process of preeclampsia. The purpose of this study was to detect miR-155 and CXCR4 m in placental tissues of pregnant women with severe preeclampsia and normal control group by real time fluorescence quantitative PCR (Real-time PCR). The expression level of RNA, using the experimental method of streptomycin biotin peroxidase complex (SABC) immuno histochemistry, using high flux detection tool - the localization and expression of CXCR4 protein in placental tissue of preeclampsia and normal control group by high flux detection tool, and preliminarily discuss the MI in placenta tissue The expression of R-155 and CXCR4 and the relationship with preeclampsia. Data and methods 1 general data 1.1 Real-time PCR subjects were enrolled in obstetrics and Gynecology of Henan maternity and child health care hospital from November 2015 to 2016, and 30 cases of pregnant women with severe preeclampsia and cesarean section were selected as experimental group (s PE). The placental tissue of 30 healthy pregnant women in the same period as the normal control group (group N) was used to detect the expression of M RNA expression.1.2 placenta tissue chip. The same subject group was collected in the Third Affiliated Hospital of Zhengzhou University from December 12, 2007 to December 31, 2010, and the clinical diagnosis was pre eclampsia before the pre eclampsia. The placental tissue of 80 pregnant women with cesarean section and 58 healthy pregnant women with cesarean section due to social factors in the same period, and the placental tissue of 58 healthy pregnant women in the same period due to social factors, the placental tissue chip was used to analyze the location and expression of CXCR4 protein by the maternal and child health transformation Medical Engineering Laboratory of Henan province. The diagnostic criteria for the diagnosis and inclusion of standard preeclampsia and its different classifications were strictly according to the diagnostic standard of the eighth edition of Obstetrics and Gynecology published by the people's Health Press. The whole pregnancy blood pressure was lower than 140/90 mm Hg, no urinary protein appeared, no maternal body, surgical and autoimmune disease and other complications, and no other obstetric complications were found. The abnormal conditions of the fetus were included in the normal control group. All the subjects included in this experiment were single pregnancy. The.2 method of cesarean delivery was used to detect the appearance of miR-155 and CXCR4 in the placental tissues of the pregnant women and the normal control group by real time fluorescence quantitative PCR technique, and the Spearman method was used to analyze the experimental group. The correlation between the expression of miR-155 and CXCR4 m RNA in pregnant women's placenta and the immunohistochemical staining of streptomycin biotin peroxidase complex (SABC method) was used to detect the placental chorionic trophoblast (VCT) tissue microarray of the maternal and child health transformation Medical Engineering Laboratory of Henan province. The expression of CXCR4 protein in 42 cases of normal control, 56 cases of preeclampsia and EVCT (29 cases of normal control, 47 cases of preeclampsia) were analyzed by SPSS 22 statistical software. The measurement data were expressed as mean standard deviation ((?) + s), and 2- delta was used. The results of the Real time-PCR experiment were analyzed by the delta Ct method. After testing the normal distribution of the data and the homogeneity of variance, both of them used the two independent sample t test. The correlation analysis adopted the Spearman correlation analysis. The experimental results of the immunohistochemistry were the hierarchical data of hierarchical classification, the rank sum test was used for statistics, and the results were alpha =0.05 as the test level. 1 the experimental group and the normal control group of pregnant women's clinical characteristics of the experimental group (Group s PE) between the age of 22~35, the average age of 27.7 + 2.8 years old, the normal control group (group N) of pregnant women age between 20~32 years, the average of 28.8 + 2.5 years old.S PE group of birth weight index (Body Msaa Index, BMI), labor cycle, systolic pressure, diastolic pressure, mean arterial pressure The birth weight of the newborns was 21.2 + 2.9 kg/m2,37.2 + 2.7 weeks, 169.5 + 17.6 mm Hg, 112.4 + 15.7mm Hg, 131.5 + 15.6 mm Hg, 2210.6 + 905.1g, 5.6 + 2.6 g/L., while the normal control group was 22.2 + mm Hg. The difference between the two groups was not statistically significant (P0.05), and the experimental group was significantly higher than the normal control group in the aspects of systolic pressure, diastolic pressure, mean arterial pressure and urine protein. In the birth weight of the newborn, the experimental group was lower than the normal control group, and the difference was statistically significant (P0.05).2 miR-155 and CXCR4 m RNA in the real group (P0.05). The expression of miR-155, CXCR4 m RNA in the placental tissues of the pregnant women and the normal control group was 1.53 + 0.92,0.87 0.73 and 0.54 + 0.38,1.53 + 0.73 respectively in the experimental group and the normal control group. The two groups were statistically significant (P0.05).3 miR-155 and CXCR4 m RNA in the experimental group of pregnant women placenta group The relative expression of miR-155 expression in the placental tissue of the experimental group was 1.53 + 0.92, and the relative expression of CXCR4m RNA in the placental tissue of the experimental group was 0.54 + 0.38. The correlation analysis of miR-155 and CXCR4 m RNA in the placental tissue of the experimental group was analyzed by Spearman test. The results showed that the two were significantly negative. Expression of.4 CXCR4 protein (r=? 0.773, P0.05) in placental VCT and EVCT tissue microarray and localization of CXCR4 protein in the cell membrane and cytoplasm of placental trophoblast and villous trophoblast cell and cytoplasm. Analysis of all site staining results showed that in placental VCT tissue microarray, CXCR4 protein was in preeclampsia group The positive expression rate was 48.21% (27/56), the positive expression rate of the normal control group was 83.33% (35/42), and the difference between the two groups was statistically significant (U=715.5, P0.05), and the positive rate of CXCR4 protein in the preeclampsia group was 48.94% (23/47) and 79.31% (23/29) in the normal control group, and the difference was statistically significant (U=3). 62, P0.05). Conclusion miR-155 in placenta of severe preeclampsia may be involved in the pathogenesis of preeclampsia by regulating the expression of CXCR4.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
本文编号：2146348
[Abstract]:Preeclampsia (Preeclampsia, PE) is one of the common internal complications of pregnancy, which is one of the types of hypertensive disorders in pregnancy. It is more common in obstetrics. The main point of diagnosis is that after 20 weeks of pregnancy, the first occurrence of systolic pressure of more than 140mm Hg and (or) diastolic pressure is more than 90mm Hg, plus one of the following new conditions: proteinuria (> 300mg/24h, or random). Urinary protein +), maternal organ dysfunction (including renal dysfunction, liver damage, neurological or blood system complications) and uterine placenta dysfunction (which may lead to fetal growth restriction). Globally, more than 1 million women die of pregnancy related diseases in low and middle income countries, and about 10% of women are in pregnancy. Between the preeclampsia and the preeclampsia, 2%~8%. takes into account that the placenta is between the mother and the fetus, and is an important organ to maintain the fetal intrauterine growth and development, based on the fact that the PE clinical symptoms can be quickly relieved or self healing after the delivery of the mother body from the mother body. It is speculated that the pathogenesis of preeclampsia is closely related to the pathophysiological changes of the placenta. Most scholars believe that the placental structure is more complex, trophoblast is the main cell type, and the dysfunction of trophoblast will lead to the invasion of blood vessels in pregnant women, that is, the insufficiency of the uterine spiral artery, the decrease of the uterine placenta blood flow, the ischemia and hypoxia of the placenta, and the release of some cytokines into the mother body. Circulation, which damages the vascular endothelial cells of pregnant women. The related pathogenesis also involves heredity, nutrition, inflammation and insulin resistance. But at present, the specific pathogenesis of preeclampsia remains unclear..MicroRNA (miRNA) is a single strand RNA of a class of 19~25 nucleotide non coding proteins, which inhibits messenger R at the post transcriptional level. The expression or degradation of NA (m RNA). Since the researchers successfully identified 3707 mature miRNAs, the miRNA database has been established. Bioinformatics analysis shows that more than 30% of the human gene may be regulated by miRNAs. Recently, more and more evidence suggests that the abnormal expression of miRNAs in the placenta is associated with the onset of preeclampsia. Chemokine receptor 4 (CXCR4) is a specific receptor for chemokine matrix cell derived factor -1 (stromal cell-derived factor-1, SDF-1, and CXCL12). Both have strong affinity and usually form a SDF-1/CXCR4 biological axis to play physiological effects. Also involved in a variety of processes, such as angiogenesis, hematopoiesis, embryonic development, and tumor invasion and metastasis. Other scholars have found that CXCR4 may be a target gene for miR-155 in cancer related studies. MiR-155 affects the angiogenesis of cancer and the function of cancer cells by regulating the expression of CXCR4 gene, and then participates in the occurrence of cancer. However, the expression of miR-155 and CXCR4 and how to participate in the pathogenesis of preeclampsia have not been reported in the process of preeclampsia. The purpose of this study was to detect miR-155 and CXCR4 m in placental tissues of pregnant women with severe preeclampsia and normal control group by real time fluorescence quantitative PCR (Real-time PCR). The expression level of RNA, using the experimental method of streptomycin biotin peroxidase complex (SABC) immuno histochemistry, using high flux detection tool - the localization and expression of CXCR4 protein in placental tissue of preeclampsia and normal control group by high flux detection tool, and preliminarily discuss the MI in placenta tissue The expression of R-155 and CXCR4 and the relationship with preeclampsia. Data and methods 1 general data 1.1 Real-time PCR subjects were enrolled in obstetrics and Gynecology of Henan maternity and child health care hospital from November 2015 to 2016, and 30 cases of pregnant women with severe preeclampsia and cesarean section were selected as experimental group (s PE). The placental tissue of 30 healthy pregnant women in the same period as the normal control group (group N) was used to detect the expression of M RNA expression.1.2 placenta tissue chip. The same subject group was collected in the Third Affiliated Hospital of Zhengzhou University from December 12, 2007 to December 31, 2010, and the clinical diagnosis was pre eclampsia before the pre eclampsia. The placental tissue of 80 pregnant women with cesarean section and 58 healthy pregnant women with cesarean section due to social factors in the same period, and the placental tissue of 58 healthy pregnant women in the same period due to social factors, the placental tissue chip was used to analyze the location and expression of CXCR4 protein by the maternal and child health transformation Medical Engineering Laboratory of Henan province. The diagnostic criteria for the diagnosis and inclusion of standard preeclampsia and its different classifications were strictly according to the diagnostic standard of the eighth edition of Obstetrics and Gynecology published by the people's Health Press. The whole pregnancy blood pressure was lower than 140/90 mm Hg, no urinary protein appeared, no maternal body, surgical and autoimmune disease and other complications, and no other obstetric complications were found. The abnormal conditions of the fetus were included in the normal control group. All the subjects included in this experiment were single pregnancy. The.2 method of cesarean delivery was used to detect the appearance of miR-155 and CXCR4 in the placental tissues of the pregnant women and the normal control group by real time fluorescence quantitative PCR technique, and the Spearman method was used to analyze the experimental group. The correlation between the expression of miR-155 and CXCR4 m RNA in pregnant women's placenta and the immunohistochemical staining of streptomycin biotin peroxidase complex (SABC method) was used to detect the placental chorionic trophoblast (VCT) tissue microarray of the maternal and child health transformation Medical Engineering Laboratory of Henan province. The expression of CXCR4 protein in 42 cases of normal control, 56 cases of preeclampsia and EVCT (29 cases of normal control, 47 cases of preeclampsia) were analyzed by SPSS 22 statistical software. The measurement data were expressed as mean standard deviation ((?) + s), and 2- delta was used. The results of the Real time-PCR experiment were analyzed by the delta Ct method. After testing the normal distribution of the data and the homogeneity of variance, both of them used the two independent sample t test. The correlation analysis adopted the Spearman correlation analysis. The experimental results of the immunohistochemistry were the hierarchical data of hierarchical classification, the rank sum test was used for statistics, and the results were alpha =0.05 as the test level. 1 the experimental group and the normal control group of pregnant women's clinical characteristics of the experimental group (Group s PE) between the age of 22~35, the average age of 27.7 + 2.8 years old, the normal control group (group N) of pregnant women age between 20~32 years, the average of 28.8 + 2.5 years old.S PE group of birth weight index (Body Msaa Index, BMI), labor cycle, systolic pressure, diastolic pressure, mean arterial pressure The birth weight of the newborns was 21.2 + 2.9 kg/m2,37.2 + 2.7 weeks, 169.5 + 17.6 mm Hg, 112.4 + 15.7mm Hg, 131.5 + 15.6 mm Hg, 2210.6 + 905.1g, 5.6 + 2.6 g/L., while the normal control group was 22.2 + mm Hg. The difference between the two groups was not statistically significant (P0.05), and the experimental group was significantly higher than the normal control group in the aspects of systolic pressure, diastolic pressure, mean arterial pressure and urine protein. In the birth weight of the newborn, the experimental group was lower than the normal control group, and the difference was statistically significant (P0.05).2 miR-155 and CXCR4 m RNA in the real group (P0.05). The expression of miR-155, CXCR4 m RNA in the placental tissues of the pregnant women and the normal control group was 1.53 + 0.92,0.87 0.73 and 0.54 + 0.38,1.53 + 0.73 respectively in the experimental group and the normal control group. The two groups were statistically significant (P0.05).3 miR-155 and CXCR4 m RNA in the experimental group of pregnant women placenta group The relative expression of miR-155 expression in the placental tissue of the experimental group was 1.53 + 0.92, and the relative expression of CXCR4m RNA in the placental tissue of the experimental group was 0.54 + 0.38. The correlation analysis of miR-155 and CXCR4 m RNA in the placental tissue of the experimental group was analyzed by Spearman test. The results showed that the two were significantly negative. Expression of.4 CXCR4 protein (r=? 0.773, P0.05) in placental VCT and EVCT tissue microarray and localization of CXCR4 protein in the cell membrane and cytoplasm of placental trophoblast and villous trophoblast cell and cytoplasm. Analysis of all site staining results showed that in placental VCT tissue microarray, CXCR4 protein was in preeclampsia group The positive expression rate was 48.21% (27/56), the positive expression rate of the normal control group was 83.33% (35/42), and the difference between the two groups was statistically significant (U=715.5, P0.05), and the positive rate of CXCR4 protein in the preeclampsia group was 48.94% (23/47) and 79.31% (23/29) in the normal control group, and the difference was statistically significant (U=3). 62, P0.05). Conclusion miR-155 in placenta of severe preeclampsia may be involved in the pathogenesis of preeclampsia by regulating the expression of CXCR4.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
【参考文献】
相关期刊论文 前1条
1 许良中，，杨文涛;免疫组织化学反应结果的判断标准[J];中国癌症杂志;1996年04期
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