透明质酸阴道凝胶在去势大鼠阴道上皮作用探讨
发布时间：2018-07-29 20:21
【摘要】：阴道萎缩是一种常见的主诉，尤其是在绝经后的妇女。阴道萎缩严重影响妇女的生活质量，，目前治疗阴道萎缩主要通过激素治疗为主，但是激素治疗会带来诸多不良反应。 目的：探讨透明质酸阴道凝胶（海罗芬）在去势大鼠中的作用。 方法：实验选用雌性SD大鼠60只，随机分为3组，分别为去势+海罗芬组（OVX+Hyalofemme），单独去势组（OVX）以及假手术组（Sham-OVX）。去势+海罗芬组以及单独去势组共40只大鼠行卵巢切除术，假手术组20只大鼠切除等量脂肪组织。术后3天开始阴道脱落细胞检测，直到阴道上皮细胞没有动情周期改变。去势+海罗芬组给予海罗芬阴道给药，单独去势组以及假手术组给予空白凝胶阴道给药。两周后取阴道分泌物行阴道微环境检测，后处死大鼠，取出子宫及阴道，称取子宫重量，阴道上皮行HE染色、透射电镜、P-AKt、VEGF的检测。 结果：1、去势+海罗芬组和单独去势组子宫重量低于假手术组，差异有统计学意义（p＜0.05）；去势+海罗芬组与单独去势组子宫重量相比，无显著性差异。2、光镜下见去势+海罗芬组大鼠阴道上皮厚于单独去势组，差异有统计学意义（p㩳0.05）；单独去势组中大鼠阴道上皮薄于假去势组，差异有统计学意义（p㩳0.05）；假去势组中大鼠阴道上皮厚于去势+海罗芬组，差异有统计学意义（p㩳0.05）。3、电镜下可见去势+海罗芬组中阴道上皮细胞排列紧密，细胞桥粒及张力微丝丰富，内质网及核糖体量多；单独去势组中阴道上皮细胞排列疏松，细胞间隙较宽，核染色质边集，可见核固缩，核碎裂及核溶解；假手术组中阴道上皮细胞表现与去势+海罗芬组相似。4、大鼠阴道分泌物检测显示，去势+海罗芬组白细胞酯酶、β-葡萄糖醛酸酶和凝固酶表达水平较假单独去势组相似，在统计学差异；单独去势组中白细胞酯酶、β-葡萄糖醛酸酶和凝固酶表达水平与假去势组和去势+海罗芬组相比较高，差异有统计学意义（p＜0.01）；假去势组中白细胞酯酶、β-葡萄糖醛酸酶和凝固酶表达水平较低。5、Western blot检测各组阴道组织P-AKt和VEGF的表达：去势+海罗芬组与假去势组相比P-AKt和VEGF的表达相似；与去势+海罗芬组和假手术组相比，单独去势组P-AKt和VEGF的表达明显降低。 结论：透明质酸阴道凝胶可以有效的缓解阴道萎缩。透明质酸阴道凝胶可以改善阴道局部微环境，同时也可以改善阴道上皮的修复能力。
[Abstract]:Vaginal atrophy is a common complaint, especially in postmenopausal women. Vaginal atrophy seriously affects the quality of life of women. At present, vaginal atrophy is mainly treated by hormone therapy, but hormone therapy will bring many adverse reactions. Objective: to investigate the role of hyaluronic acid vaginal gel (Herofine) in ovariectomized rats. Methods: sixty female Sprague-Dawley rats were randomly divided into three groups: castration group, (OVX Hyalofemme), group, (OVX) group and sham operation group (Sham-OVX). Ovariectomy was performed in 40 rats in ovariectomized group and ovariectomized group in 20 rats in sham-operated group. Vaginal exfoliative cells were detected 3 days after operation until vaginal epithelial cells did not change estrous cycle. The ovariectomized group was given hydrophen vaginal administration, the single castrated group and the sham operation group were given blank gel vaginal administration. Two weeks later, vaginal secretions were taken for vaginal microenvironment detection, then rats were killed, uterus and vagina were taken out, uterine weight was taken out, vaginal epithelium was stained with HE, and VEGF was detected by transmission electron microscope (TEM). Results the weight of uterus in castration group and single castration group was significantly lower than that in sham operation group (p < 0.05), and the uterine weight in castration group was higher than that in castrated group (p < 0.05), and the uterine weight in castration group was significantly lower than that in ovariectomized group (p < 0.05). There was no significant difference in the thickness of vaginal epithelium between castrated group and ovariectomized group under light microscope (p0.05), but the thickness of vaginal epithelium in castration group was thinner than that in sham castration group (p0.05). The vaginal epithelium in ovariectomized group was thicker than that in ovariectomized group, the difference was statistically significant (p0.05) .3The results of electron microscope showed that the vaginal epithelial cells in ovariectomized group were closely arranged, the desmosomes and tension microfilaments were abundant, and the amount of endoplasmic reticulum and ribosome was more. In the castrated group, the vaginal epithelial cells were arranged loosely, the intercellular space was wide, the chromatin edge was gathered, the nuclei were pyknosis, nuclear fragmentation and nuclear dissolution. The expression of leucocyte esterase, 尾 -glucuronidase and coagulase in ovariectomized group was similar to that in ovariectomized group, and the expression of leucocyte esterase, 尾 -glucuronidase and coagulase in castrated group was similar to that in ovariectomized group. The expression levels of leucocyte esterase, 尾 -glucuronidase and coagulase in castration group were significantly higher than those in sham castration group and ovariectomized group (p < 0.01). The expression levels of leucocyte esterase, 尾 -glucuronidase and coagulase were lower in ovariectomized group. 5Western blot was used to detect the expression of P-AKt and VEGF in vaginal tissue of each group. The expression of P-AKt and VEGF in castrated group was similar to that in pseudocastrated group. The expression of P-AKt and VEGF in ovariectomized group was significantly lower than that in ovariectomized group and sham-operated group. Conclusion: hyaluronic acid vaginal gel can effectively relieve vaginal atrophy. Hyaluronic acid vaginal gel can improve local microenvironment and repair ability of vaginal epithelium.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.74
本文编号：2153897
[Abstract]:Vaginal atrophy is a common complaint, especially in postmenopausal women. Vaginal atrophy seriously affects the quality of life of women. At present, vaginal atrophy is mainly treated by hormone therapy, but hormone therapy will bring many adverse reactions. Objective: to investigate the role of hyaluronic acid vaginal gel (Herofine) in ovariectomized rats. Methods: sixty female Sprague-Dawley rats were randomly divided into three groups: castration group, (OVX Hyalofemme), group, (OVX) group and sham operation group (Sham-OVX). Ovariectomy was performed in 40 rats in ovariectomized group and ovariectomized group in 20 rats in sham-operated group. Vaginal exfoliative cells were detected 3 days after operation until vaginal epithelial cells did not change estrous cycle. The ovariectomized group was given hydrophen vaginal administration, the single castrated group and the sham operation group were given blank gel vaginal administration. Two weeks later, vaginal secretions were taken for vaginal microenvironment detection, then rats were killed, uterus and vagina were taken out, uterine weight was taken out, vaginal epithelium was stained with HE, and VEGF was detected by transmission electron microscope (TEM). Results the weight of uterus in castration group and single castration group was significantly lower than that in sham operation group (p < 0.05), and the uterine weight in castration group was higher than that in castrated group (p < 0.05), and the uterine weight in castration group was significantly lower than that in ovariectomized group (p < 0.05). There was no significant difference in the thickness of vaginal epithelium between castrated group and ovariectomized group under light microscope (p0.05), but the thickness of vaginal epithelium in castration group was thinner than that in sham castration group (p0.05). The vaginal epithelium in ovariectomized group was thicker than that in ovariectomized group, the difference was statistically significant (p0.05) .3The results of electron microscope showed that the vaginal epithelial cells in ovariectomized group were closely arranged, the desmosomes and tension microfilaments were abundant, and the amount of endoplasmic reticulum and ribosome was more. In the castrated group, the vaginal epithelial cells were arranged loosely, the intercellular space was wide, the chromatin edge was gathered, the nuclei were pyknosis, nuclear fragmentation and nuclear dissolution. The expression of leucocyte esterase, 尾 -glucuronidase and coagulase in ovariectomized group was similar to that in ovariectomized group, and the expression of leucocyte esterase, 尾 -glucuronidase and coagulase in castrated group was similar to that in ovariectomized group. The expression levels of leucocyte esterase, 尾 -glucuronidase and coagulase in castration group were significantly higher than those in sham castration group and ovariectomized group (p < 0.01). The expression levels of leucocyte esterase, 尾 -glucuronidase and coagulase were lower in ovariectomized group. 5Western blot was used to detect the expression of P-AKt and VEGF in vaginal tissue of each group. The expression of P-AKt and VEGF in castrated group was similar to that in pseudocastrated group. The expression of P-AKt and VEGF in ovariectomized group was significantly lower than that in ovariectomized group and sham-operated group. Conclusion: hyaluronic acid vaginal gel can effectively relieve vaginal atrophy. Hyaluronic acid vaginal gel can improve local microenvironment and repair ability of vaginal epithelium.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.74
【参考文献】
相关期刊论文 前2条
1 杨素珍;阚洪玲;张天民;;透明质酸在美容化妆品方面的应用[J];食品与药品;2010年07期
2 于学丽,王传栋,李保陆,曹成波;透明质酸的改性及其应用[J];生物医学工程研究;2005年01期
本文编号：2153897
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