低功率超声放大卵巢癌肿瘤标志物的实验研究

发布时间：2018-08-02 12:05

【摘要】：目的:探讨应用低功率超声放大卵巢癌肿瘤标志物提高卵巢癌早期检出率的可行性。方法:用自制的超声信号发生装置,设定超声信号频率为1 MHz,超声功率为0.1~0.3 W/cm~2,时间为1、2、3、4、5分钟,对人卵巢癌细胞株SKOV3行超声辐射,检测培养基中的肿瘤标志物;以功率0.1~0.3 W/cm~2和超声辐射时间5、10、15分钟对裸鼠卵巢癌瘤体进行声辐射后检测血肿瘤标志物,对标志物的放大效应进行分析。结果:在细胞水平,超声辐射时间一定的条件下,CA125浓度随功率增大而增加,且差异有统计学意义(P0.05)。0.2 W/cm~2和0.3 W/cm~2超声条件下,CA125浓度随照射时间延长而增加,不同时间组间差异均有统计学意义(P0.05)。相同的超声辐射时间条件下,CA199浓度随功率的提高而增加(P0.05)。经超声辐射后,各组裸鼠血清CA_(125)、CA_(199)浓度较对照组均显著上升(P0.05),不同功率组间差异有统计学意义(P0.05)。结论:低功率超声可在细胞和瘤体使卵巢癌肿瘤标志物CA_(125)和CA_(199)浓度增加。
[Abstract]:Objective: To explore the feasibility of improving the early detection rate of ovarian cancer by using low power ultrasound amplification of ovarian cancer tumor markers. Methods: using the self-made ultrasonic signal generator, the ultrasonic signal frequency is 1 MHz, the ultrasonic power is 0.1~0.3 W/cm~2 and the time is 1,2,3,4,5 minutes. The ultrasonic radiation of the human ovarian cancer cell line SKOV3 is performed and the culture medium is detected. The tumor markers in the tumor were detected with the power 0.1~0.3 W/cm~2 and the time of ultrasonic radiation of 5,10,15 minutes to detect the tumor markers of the ovarian cancer in nude mice. The amplification effect of the markers was analyzed. The results showed that the concentration of CA125 increased with the increase of power at the cell level and the time of ultrasonic radiation, and the difference was unified. Under the condition of P0.05.0.2 W/cm~2 and 0.3 W/cm~2 ultrasound, the concentration of CA125 increased with the prolonged irradiation time, and the difference between different time groups had statistical significance (P0.05). The concentration of CA199 increased with the increase of power (P0.05) under the same ultrasonic time condition. The serum CA_ (125), CA_ (199) concentration of the nude mice after ultrasonic radiation Compared with the control group (P0.05), there was a significant difference between different power groups (P0.05). Conclusion: low power ultrasound can increase the concentration of ovarian cancer tumor markers CA_ (125) and CA_ (199) in cells and tumor bodies.
【作者单位】：东南大学附属中大医院;东南大学公共卫生学院;南京医科大学附属南京医院;苏州大学附属第二医院;江苏省盱眙县人民医院;
【基金】：江苏省预防医学研究课题(编号:Y2013067)
【分类号】：R737.31

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