STAT4基因rs10181656位点的多态性与不明原因复发性自然流产的相关性

发布时间：2018-08-05 11:17

【摘要】：【目的】探讨信号转导与转录激活因子4(STAT4)基因在不明原因复发性自然流产(URSA)表达及其表达量与rs10181656位点多态性的关系。【方法】分别采用聚合酶链反应-限制性片段长度多态性法检测332名URSA患者和260名健康对照者rs10181656位点的基因型频率;应用免疫组织化学技术检测其中的86名URSA患者及77名健康对照者蜕膜STAT4基因的蛋白表达量。【结果】rs10181656C/G:URSA组中C/C、C/G及G/G基因型频率分别是36.45%、46.99%及16.57%,而对照组中的3种基因型的频率分别是46.54%、45.38%及8.08%,具有统计学差异(P0.05),G等位基因增加URSA组发病风险(OR=1.50,P0.05)。STAT4蛋白表达量在URSA组和对照组中的差异具有统计学意义(P0.05);在URSA组内分为C/C、C/G及G/G基因型的三个亚组STAT4蛋白表达:三个亚组的STAT4蛋白表达差异具有统计学意义(P0.05),在对照组中亦然(P0.05),具有G/G基因型样本STAT4蛋白高表达于C/C基因型(P均0.05);相同基因型在URSA组和对照组中STAT4蛋白表达:C/C基因型样本在URSA组和对照组中STAT4蛋白的表达差异无统计学意义(P0.05),C/G及G/G基因型样本亦然(P0.05)。【结论】rs10181656位点多态性通过影响STAT4蛋白表达,从而与增加URSA发病风险相关。
[Abstract]:[Objective] to investigate the relationship between the expression of signal transduction and activator 4 (STAT4) gene in the expression of unexplained recurrent spontaneous abortion (URSA) and its expression and the polymorphism of rs10181656 loci. [Methods] 332 URSA patients and 260 healthy controls were detected by polymerase chain reaction restriction fragment length polymorphism (PCR), respectively. The genotype frequency of the loci; the protein expression of the decidua STAT4 gene in 86 URSA patients and 77 healthy controls was detected by immunohistochemistry. [results] the genotype frequencies of C/C, C/G and G/G in the rs10181656C/G:URSA group were 36.45%, 46.99%, and 16.57% respectively, while the frequencies of 3 genotypes in the group were 46.54%, 45., respectively. 38% and 8.08%, with statistical difference (P0.05), G allele increased the risk of URSA group (OR=1.50, P0.05).STAT4 protein expression in the URSA group and the control group with statistical significance (P0.05), in the URSA group is divided into C/C, C/G and G/G genotypes of the three subgroups of protein expression: Three subgroups of differences in protein expression P0.05, as well as in the control group (P0.05), the STAT4 protein with G/G genotype was highly expressed in the C/C genotype (P 0.05), and the same genotype was expressed in the URSA group and the control group. The C/C genotype samples had no significant difference in the expression of STAT4 protein in the URSA group and the control group (P0.05). (P0.05) [Conclusion] rs10181656 polymorphism is associated with increased risk of URSA through affecting STAT4 protein expression.
【作者单位】：中山大学附属第一医院妇产科;
【基金】：广东省自然科学基金(S2011010003522)
【分类号】：R714.21

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