PDCD5在子宫内膜癌中的表达及临床意义

发布时间：2018-08-08 14:56

【摘要】：目的：本文旨在通过检测PDCD5 (programmed cell death 5,程序性细胞死亡因子5)在子宫内膜癌(Endometrial carcinoma, EC)组织中的表达水平,结合临床资料,并分析其意义,初步探讨PDCD5的表达与子宫内膜癌发生的相关性。方法：收集2012年5月至2013年2月于滨州医学院附属医院妇科行妇科手术,且均经病理学检查证实为EC为研究对象,总标本例数n=60；另随机选取同期行妇科手术中所取得子宫内膜不典型增生(endometrial atypical hyperplasia, EAH)组织(n=28)和正常增生期子宫内膜(normal proliferative endometrium, NPE)组织(n=28)作为对照。分别采用实时荧光定量PCR(Realtime fluores-cence quantitative PCR, Real time PCR)和免疫组织化学法(Immunohistochemical, IHC)检测PDCD5 mRNA和蛋白的表达水平,并分析其与子宫内膜癌病理特征的关系。本实验所遵守的标准为滨州医学院附属医院伦理委员会所制定的相关伦理学标准。结果：子宫内膜癌中PDCD5 mRNA的表达明显低于对照组,且差异有统计学意义,P≤0.05。PDCD5蛋白在EC组织中的阳性表达率为45.0%,显著低于EAH及NPE组织中的阳性表达率(P≤0.05)。而且结果显示PDCD5的表达水平和EC患者的临床分期、组织学分级、肌层浸润以及有无淋巴结转移有相关性(均P≤0.05),而与子宫内膜癌患者的年龄未发现明显的关联性(P0.05)。结论:采用实时荧光定量PCR和免疫组织化学技术检测子宫内膜癌组织中PDCD5的表达,发现PDCD5在子宫内膜癌组织中的表达较对照组织中的表达降低甚至缺失。且PDCD5的表达水平与子宫内膜癌的临床病理参数密切相关：临床分期越高,PDCD5表达越低；组织学分级越高,PDCD5蛋白的表达越低；肌层浸润越深,PDCD5表达越低；淋巴结有转移,PDCD5表达越低；PDCD5表达与年龄无关。PDCD5有可能成为一种潜在的子宫内膜癌的早期诊断分子标记物。
[Abstract]:Objective: to investigate the expression of PDCD5 (programmed cell death 5 and programmed cell death factor 5 (PDCD5 (programmed cell death 5) in endometrial carcinoma (Endometrial carcinoma, EC) and to analyze their clinical significance. To explore the correlation between the expression of PDCD5 and the occurrence of endometrial carcinoma. Methods: from May 2012 to February 2013, gynecological operations were performed in affiliated Hospital of Binzhou Medical College, and were confirmed as EC by pathological examination. Another randomly selected endometrial atypical hyperplasia (endometrial atypical hyperplasia, EAH) tissue (nm28) and normal proliferative endometrial (normal proliferative endometrium, NPE) tissue (nm28) obtained during gynecological surgery were used as control group. The expression of PDCD5 mRNA and protein was detected by real-time fluorescence quantitative PCR (Realtime fluores-cence quantitative PCR, Real time PCR) and immunohistochemical (Immunohistochemical, IHC), and the relationship between the expression of PDCD5 mRNA and the pathological features of endometrial carcinoma was analyzed. The standard of this experiment is the ethical standard of the affiliated Hospital Ethics Committee of Binzhou Medical College. Results: the expression of PDCD5 mRNA in endometrial carcinoma was significantly lower than that in control group, and the positive expression rate of P 鈮，

本文编号：2172141