外源性甲状腺素对胎儿酒精效果模型仔鼠生长及神经系统发育的影响

发布时间：2018-08-13 16:15

【摘要】：目的探讨外源性甲状腺素对胎儿酒精效果(FAE)模型仔鼠脑损伤的改善作用。方法取SD孕鼠18只,随机分为对照组、模型组、治疗组,每组6只。从受孕第6天开始至分娩,模型组、治疗组每日摄取22%乙醇20 m L,对照组每日摄取与模型组相同热卡、含8%糖的牛奶。各组分娩后保留仔鼠10只,并与母鼠分离,由代理母鼠养育。对照组、模型组仔鼠第1~10天每日颈后皮下注射生理盐水2μL/g,治疗组仔鼠每日颈后皮下注射2.5μg/m L L-甲状腺素2μL/g。观察各组仔鼠出生第7、14、21、28天体质量、血浆甲状腺素水平、脑组织脑源性神经营养因子(BDNF)含量及BDNF阳性浦肯野细胞变化。结果治疗组和对照组仔鼠出生各时间点体质量、血浆甲状腺素水平及脑组织BDNF含量均明显高于模型组同期(P均0.01),而治疗组和对照组仔鼠出生各时间点比较P均0.05。治疗组和对照组仔鼠出生第7天开始小脑皮质中均可观察到排列整齐的BDNF阳性浦肯野细胞,且出生第28天时治疗组较对照组BDNF阳性浦肯野细胞数量更多,排列更整齐,突起更长,并可见部分分支。模型组各时间点均可观察到延迟增长和分化的浦肯野细胞。结论外源性甲状腺素可纠正FAE模型仔鼠的低甲状腺素状态,抑制BDNF阳性浦肯野细胞变性,改善FAE导致的神经系统发育障碍。
[Abstract]:Objective to investigate the effect of exogenous thyroxine on brain injury in (FAE) model. Methods 18 pregnant SD rats were randomly divided into control group, model group and treatment group. From the 6th day of conception to delivery, the model group and the treatment group received 22% ethanol 20 mL daily, while the control group received the same hot calorie and 8% sugar milk daily as the model group. After delivery, 10 rats in each group were kept and separated from the mother mice, which were nurtured by the surrogate mother. In the control group, rats in the model group were subcutaneously injected with normal saline 2 渭 L / g per day for 10 days, and those in the treatment group were subcutaneously injected with 2.5 渭 g / mL L-L-thyroxine 2 渭 L / g per day. The body mass, plasma thyroxine level, brain-derived neurotrophic factor (BDNF) content and BDNF positive Purkinje cells were observed. Results the body weight, plasma thyroxine level and BDNF content of brain tissue in the treatment group and the control group were significantly higher than those in the model group at each time point (P0.01), but the difference between the treatment group and the control group was 0.05. BDNF positive Purkinje cells were observed in cerebellar cortex of treatment group and control group on the 7th day of birth, and the number and arrangement of BDNF positive Purkinje cells in treatment group were more and more neat than those in control group on the 28th day of birth. The protuberance is longer and some branches are visible. Delayed growth and differentiation of Purkinje cells were observed at all time points in the model group. Conclusion exogenous thyroxine can correct the hypothyroxine state of FAE model rats, inhibit the degeneration of BDNF positive Purkinje cells, and improve the developmental disorder of nervous system induced by FAE.
【作者单位】：延边大学附属医院;
【分类号】：R-332;R714.5

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