胎膜早破产妇胎膜组织中TRAIL、MMP-2基因及蛋白表达变化

发布时间：2018-08-13 21:16

【摘要】：目的观察胎膜早破产妇胎膜组织中肿瘤坏死因子相关凋亡配体(TRAIL)、基质金属蛋白酶2(MMP-2)mRNA及蛋白的表达变化,并探讨其意义。方法选择足月胎膜早破产妇30例作为胎膜早破组,同孕期健康产妇30例作为对照组。两组产妇分娩后取胎膜组织,采用免疫组化法检测TRAIL、MMP-2蛋白,real-time PCR法检测TRAIL、MMP-2 mRNA。结果胎膜早破组胎膜组织中TRAIL蛋白表达阳性26例(87%)、MMP-2蛋白表达阳性23例(77%),对照组分别为17例(57%)、15例(50%)。两组胎膜组织中TRAIL、MMP-2蛋白阳性表达率相比,P均0.05。胎膜早破组胎膜组织中TRAIL、MMP-2 mRNA相对表达量分别为1.62±0.18、1.78±0.54,对照组分别为0.99±0.17、1.03±0.49。两组胎膜组织中TRAIL、MMP-2 mRNA相对表达量相比,P均0.05。结论胎膜早破产妇胎膜组织中TRAIL、MMP-2蛋白阳性表达率升高,TRAIL、MMP-2 mRNA表达上调。TRAIL、MMP-2高表达可能参与了胎膜早破发病过程。
[Abstract]:Objective to investigate the expression of (TRAIL), matrix metalloproteinase 2 (MMP-2) mRNA and protein in fetal membranes of premature rupture of fetal membranes and its significance. Methods 30 cases of term premature rupture of membranes were selected as the group of premature rupture of membranes, and 30 cases of healthy pregnant women as control group. The fetal membrane tissue was taken from the parturient of the two groups and the TRAILP MMP-2 protein was detected by immunohistochemistry and real-time PCR method was used to detect the TRAILP MMP-2 mRNA. Results the expression of TRAIL protein was positive in 26 cases (87%) in the premature rupture group and in 17 cases (57%) in the control group. The positive expression rate of TRAILN MMP-2 protein in the fetal membranes of the two groups was 0.05 compared with that of the control group (P < 0.05). The relative expression of TRAILN MMP-2 mRNA in membranes of premature rupture group was 1.62 卤0.181.78 卤0.54, and that in control group was 0.99 卤0.170.03 卤0.49. The relative expression of TRAILP MMP-2 mRNA in the membranes of both groups was 0.05 compared with that of the control group (P < 0.05). Conclusion the positive expression rate of TRAILP MMP-2 protein in fetal membrane tissue of premature rupture of fetal membrane is increased. The up-regulated expression of TRAILP MMP-2 mRNA may be involved in the pathogenesis of premature rupture of membranes.
【作者单位】：郑州大学第三附属医院;
【分类号】：R714.433

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