联合LC-MS和化学发光免疫法分析不同类型PCOS的雄激素水平

发布时间：2018-08-14 11:43

【摘要】：目的:利用液相色谱-质谱联用(LC-MS)方法和化学发光免疫分析法测定不同临床类型PCOS患者的血清雄激素水平,分析中国PCOS女性的高雄激素血症的生化特点。方法:纳入有排卵障碍和多囊卵巢的PCOS患者149例,根据血清睾酮(T)和硫酸脱氢表雄酮(DHEAS)值及F-G多毛评分情况分为两组:高雄组55例,非高雄组94例。同期选取输卵管性不孕患者82例作为对照组。化学发光法测定性激素结合球蛋白(SHBG)水平,计算游离雄激素指数(FTI)。LC-MS法测定各组血清睾酮、雄烯二酮(AD)和脱氢表雄酮(DHEA)。结果:高雄组的血清T水平及FTI值显著高于非高雄组及对照组(P0.05),非高雄组高于对照组(P0.05);高雄组、非高雄组的DHEAS及AD水平均高于对照组(P0.05);3组的DHEA水平比较,差异均无统计学意义。结论:血清T、AD、DHEAS及FTI均为诊断PCOS高雄激素血症的敏感指标;T及FTI是PCOS分型(高雄及非高雄)的主要依据;LC-MS和化学发光免疫分析法对PCOS高雄激素血症的诊断具有一致性。
[Abstract]:Objective: To determine the serum androgen levels in different clinical types of PCOS patients by liquid chromatography-mass spectrometry (LC-MS) and chemiluminescent immunoassay, and to analyze the biochemical characteristics of hyperandrogenism in Chinese PCOS women. Epidrosterone (DHEAS) value and F-G hirsutism score were divided into two groups: 55 cases in Kaohsiung group and 94 cases in non-Kaohsiung group. 82 cases of tubal infertility were selected as control group at the same time. Sex hormone binding globulin (SHBG) level was measured by chemiluminescence method, free androgen index (FTI) was calculated. Serum testosterone, androstenedione (AD) and dehydrogenation table were determined by LC-MS. Results: Serum T and FTI levels in Kaohsiung group were significantly higher than those in non-Kaohsiung group and control group (P 0.05), and those in non-Kaohsiung group were higher than those in control group (P 0.05); DHEAS and AD levels in Kaohsiung group and non-Kaohsiung group were higher than those in control group (P 0.05); there was no significant difference in DHEA levels among three groups. Sensitive markers of hyperandrogenism; T and FTI were the main basis of PCOS typing (Kaohsiung and non-Kaohsiung); LC-MS and chemiluminescence immunoassay were consistent in the diagnosis of PCOS hyperandrogenism.
【作者单位】：武汉市第一医院妇产科;武汉农业检测所;
【基金】：武汉市科技局基金(No:2013062301010815) 武汉市卫计委基金(No:WX14C04)
【分类号】：R711.75

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