不同浓度氧气对人未成熟卵子体外成熟和早期胚胎发育的影响

发布时间：2018-08-15 12:40

【摘要】：目的收集辅助生殖治疗中废弃的人类未成熟卵子,研究其在不同浓度氧气培养条件下的卵子成熟率和受精及其后续早期胚胎发育的影响。方法将收集的未成熟卵子随机分组为低浓度和高浓度氧气条件进行体外成熟培养,将成熟卵子进行卵胞质内单精子显微注射并行体外培养,研究卵子的体外成熟率、受精率以及胚胎卵裂率和优质胚胎率。结果对于GV期来源卵子,低氧培养条件下的卵子体外成熟率和受精率要显著高于高氧组(P0.05),两组间的胚胎卵裂率和优质胚胎率比较差异无统计学意义(P0.05);MI期来源卵子在低氧培养条件下的卵子体外成熟率要显著高于高氧组(P0.05),两组间的受精率、胚胎卵裂率和优质胚胎率差异无统计学意义(P0.05)。结论低浓度氧气对于人类未成熟卵子的体外成熟有促进作用,能提高GV期卵子的体外成熟后的受精率;对于体外成熟培养卵子来源的胚胎卵裂和优质胚胎率没有影响。
[Abstract]:Objective to collect human immature eggs abandoned in assisted reproductive therapy and to study the effects of fertilization and subsequent early embryo development on the maturation rate of eggs in different concentrations of oxygen culture. Methods immature eggs were randomly divided into low concentration and high concentration oxygen conditions for in vitro maturation culture. The mature eggs were microinjected into oocyte cytoplasm and cultured in vitro to study the maturation rate of eggs in vitro. Fertilization rate, embryo cleavage rate and high quality embryo rate. Results for GV origin eggs, The maturation rate and fertilization rate of eggs in hypoxic culture were significantly higher than those in hyperoxia group (P0.05). There was no significant difference between the two groups in embryo cleavage rate and high quality embryo rate (P0.05). The rate of maturation in vitro was significantly higher than that in hyperoxia group (P0.05). There was no significant difference between embryo cleavage rate and high quality embryo rate (P0.05). Conclusion low concentration of oxygen can promote in vitro maturation of human immature eggs, increase fertilization rate of GV eggs after in vitro maturation, and have no effect on cleavage and high quality embryo rate of eggs derived from in vitro maturation and culture.
【作者单位】：广州医科大学附属第三医院生殖医学中心广东省生殖医学重点实验室;广州医科大学附属第三医院重症医学科;
【基金】：广州医科大学自然科学青年项目(编号:2015A08) 国家自然科学青年科学基金项目(编号:81502507)
【分类号】：R714.8

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