PPARγ基因Pro12Ala和C1431T多态性与妊娠期糖尿病发病的关系
发布时间：2018-08-18 13:01
【摘要】：目的探讨PPARγ基因Pro12Ala和C1431T多态性与妊娠期糖尿病(GDM)发病的关系。方法选择2015年6月至2016年2月期间在青岛大学附属医院产科门诊进行产检以及住院剖宫产的青岛地区汉族患者260名作为研究对象,采用病例对照的研究方法选取其中的130例妊娠期糖尿病孕妇作为实验组,平均孕周为(39.3±0.6)周,平均年龄为(28.6±3.1)岁,平均体重指数为(24.2±3.1);另130例健康孕妇作为对照组,平均年龄为(28.3±3.2)岁,平均孕周为(39.1±0.5)周,平均体重指数为(24.1±2.6),且两组孕妇均无高血压、心脏病等其他妊娠合并症。测定两组孕妇血清空腹血糖(FPG)、空腹胰岛素(FINS)、甘油三酯(TG)、总胆固醇(TC)及体质指数,并计算胰岛素抵抗指数的自然对数ln(HOMA-IR)。对PPARγ基因的Pro12Ala和C1431T这两个位点应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法进行酶切鉴定,最后从中选取部分标本进行送检测序,以验证得到的结果是否正确。结果(1)GDM组Ala12等位基因频率是0.054,显著低于对照组的0.092,差别有统计学意义;T1431等位基因频率是0.269,高于对照组的0.246,差别无统计学意义。位点Pro12Ala的基因型频率χ2=3.345(P0.05),等位基因频率χ2=1.420(P0.05),位点C1431T的基因型频率χ2=1.041(P0.05),等位基因频率χ2=0.181(P0.05),两个位点基因频率分布均无统计学意义(见表2.1.1、2.2.1)。(2)GDM组PPARγPro12Ala基因位点中,Pro/Pro基因型甘油三酯及空腹血糖的测量数值分别为2.85±0.89、3.92±1.06,Pro/Ala和Ala/Ala基因型甘油三酯及空腹血糖的测量数值分别为2.02±1.23、3.24±0.85,携带Ala等位基因的孕妇甘油三酯和空腹血糖水平明显低于无Ala等位基因的孕妇,差别有统计学意义;Pro/Pro基因型胆固醇、空腹胰岛素、胰岛素抵抗指数的测量数值分别为5.33±1.08、12.95±9.25、2.28±2.36,Pro/Ala和Ala/Ala基因型胆固醇、空腹胰岛素、胰岛素抵抗指数的测量数值分别为5.69±3.86、13.56±2.82、2.76±0.42,这几种指标在两种基因型中的分布无统计学差异(P0.05)。而C1431T基因位点中,GDM组C/C型各生化指标的数值为2.23±0.65、5.28±1.45、3.88±1.37、12.94±3.25、2.42±2.05,C/T+T/T型各生化指标的数值为2.53±1.78、5.59±3.68、4.11±0.69、13.27±2.96、2.56±0.29,各指标在不同基因型的携带者中对GDM的发病均未发现有明显统计学差异(P0.05)。结论PPARγ基因Pro12Ala多态性可能是抑制GDM的发病的相关因子,Ala等位基因可能与GDM糖脂代谢紊乱有关;C1431T基因多态性与GDM发病以及相关生化指标无明显相关性。
[Abstract]:Objective to investigate the relationship between Pro12Ala and C1431T polymorphisms of PPAR 纬 gene and (GDM) in gestational diabetes mellitus (GDM). Methods from June 2015 to February 2016, 260 Han nationality patients in Qingdao from June 2015 to February 2016 were selected as the subjects of the study, who underwent birth examination and inpatient cesarean section at the obstetrical outpatient department of affiliated Hospital of Qingdao University. A case-control study was conducted in 130 pregnant women with gestational diabetes mellitus (GDM) as experimental group, with an average gestational age of (39.3 卤0.6) weeks, mean age of (28.6 卤3.1) years and average body mass index (BMI) of (24.2 卤3.1), and 130 healthy pregnant women as control group, with an average age of (28.3 卤3.2) years. The mean gestational weeks were (39.1 卤0.5) weeks, and the average body mass index (BMI) was (24.1 卤2.6) weeks. There were no hypertension, heart disease and other pregnancy complications in the two groups. Serum fasting blood glucose (FPG),) fasting insulin (FINS),) triglyceride (TG),) total cholesterol (TC) and body mass index (BMI) were measured and the natural logarithm of insulin resistance index (HOMA-IR) was calculated. The Pro12Ala and C1431T loci of PPAR 纬 gene were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results (1) the frequency of Ala12 allele in GDM group was 0.054, which was significantly lower than that in control group (0.092). The allele frequency of T1431 in GDM group was 0.269, which was higher than that in control group (0.246). The genotype frequency of Pro12Ala was 3.345 (P0.05), the frequency of allele was 1.420 (P0.05), the genotype frequency of C1431T was 1.041 (P0.05), and the frequency of allele was 蠂 2 0.181 (P0.05). There was no significant difference between the two loci (see Table 2.1.12.2.1). (2) in the PPAR 纬 Pro12Ala gene locus of GDM group. The measured values of fasting blood glucose and fasting blood glucose were 2.85 卤0.89 卤3.92 卤1.06g Prop / Ala and 2.02 卤1.233.24 卤0.85, respectively. The levels of triglyceride and fasting blood glucose of pregnant women with Ala allele were significantly lower than those of pregnant women without Ala allele. There were significant differences in cholesterol, fasting insulin and insulin resistance index between Pro-Pro-Pro genotype and Ala/Ala genotype (5.33 卤1.08 卤12.95 卤9.25 卤2.28 卤2.36) and fasting insulin, respectively. The measured values of insulin resistance index were 5.69 卤3.86 and 13.56 卤2.82 卤2.76 卤0.42, respectively. There was no significant difference in the distribution of these indexes between the two genotypes (P0.05). In the C1431T gene locus, the values of each biochemical index of C / C type C type in GDM group were 2.23 卤0.65V 5.28 卤1.45N 3.88 卤1.3712.94 卤3.254.42 卤2.255C / T, 2.53 卤1.785.59 卤3.684.11 卤0.696.27 卤2.962.56 卤0.29, respectively. There was no significant difference in the incidence of GDM among the carriers with different genotypes (P0.05). Conclusion the Pro12Ala polymorphism of PPAR 纬 gene may be a related factor to inhibit the pathogenesis of GDM. The allele of Ala may not be associated with the disorder of GDM glycolipid metabolism. The polymorphism of C1431T gene has no significant correlation with the pathogenesis of GDM and related biochemical indexes.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.256
本文编号：2189542
[Abstract]:Objective to investigate the relationship between Pro12Ala and C1431T polymorphisms of PPAR 纬 gene and (GDM) in gestational diabetes mellitus (GDM). Methods from June 2015 to February 2016, 260 Han nationality patients in Qingdao from June 2015 to February 2016 were selected as the subjects of the study, who underwent birth examination and inpatient cesarean section at the obstetrical outpatient department of affiliated Hospital of Qingdao University. A case-control study was conducted in 130 pregnant women with gestational diabetes mellitus (GDM) as experimental group, with an average gestational age of (39.3 卤0.6) weeks, mean age of (28.6 卤3.1) years and average body mass index (BMI) of (24.2 卤3.1), and 130 healthy pregnant women as control group, with an average age of (28.3 卤3.2) years. The mean gestational weeks were (39.1 卤0.5) weeks, and the average body mass index (BMI) was (24.1 卤2.6) weeks. There were no hypertension, heart disease and other pregnancy complications in the two groups. Serum fasting blood glucose (FPG),) fasting insulin (FINS),) triglyceride (TG),) total cholesterol (TC) and body mass index (BMI) were measured and the natural logarithm of insulin resistance index (HOMA-IR) was calculated. The Pro12Ala and C1431T loci of PPAR 纬 gene were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results (1) the frequency of Ala12 allele in GDM group was 0.054, which was significantly lower than that in control group (0.092). The allele frequency of T1431 in GDM group was 0.269, which was higher than that in control group (0.246). The genotype frequency of Pro12Ala was 3.345 (P0.05), the frequency of allele was 1.420 (P0.05), the genotype frequency of C1431T was 1.041 (P0.05), and the frequency of allele was 蠂 2 0.181 (P0.05). There was no significant difference between the two loci (see Table 2.1.12.2.1). (2) in the PPAR 纬 Pro12Ala gene locus of GDM group. The measured values of fasting blood glucose and fasting blood glucose were 2.85 卤0.89 卤3.92 卤1.06g Prop / Ala and 2.02 卤1.233.24 卤0.85, respectively. The levels of triglyceride and fasting blood glucose of pregnant women with Ala allele were significantly lower than those of pregnant women without Ala allele. There were significant differences in cholesterol, fasting insulin and insulin resistance index between Pro-Pro-Pro genotype and Ala/Ala genotype (5.33 卤1.08 卤12.95 卤9.25 卤2.28 卤2.36) and fasting insulin, respectively. The measured values of insulin resistance index were 5.69 卤3.86 and 13.56 卤2.82 卤2.76 卤0.42, respectively. There was no significant difference in the distribution of these indexes between the two genotypes (P0.05). In the C1431T gene locus, the values of each biochemical index of C / C type C type in GDM group were 2.23 卤0.65V 5.28 卤1.45N 3.88 卤1.3712.94 卤3.254.42 卤2.255C / T, 2.53 卤1.785.59 卤3.684.11 卤0.696.27 卤2.962.56 卤0.29, respectively. There was no significant difference in the incidence of GDM among the carriers with different genotypes (P0.05). Conclusion the Pro12Ala polymorphism of PPAR 纬 gene may be a related factor to inhibit the pathogenesis of GDM. The allele of Ala may not be associated with the disorder of GDM glycolipid metabolism. The polymorphism of C1431T gene has no significant correlation with the pathogenesis of GDM and related biochemical indexes.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.256
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