CCL28通过增强MMP-2活力促进滋养细胞浸润功能

发布时间：2018-08-22 12:16

【摘要】：目的:探究趋化因子CCL28对滋养细胞生物学行为的影响及其调控机制。方法:分离培养并鉴定原代人早孕蜕膜基质细胞(DSCs),ELISA法检测人DSCs与人正常绒毛膜滋养细胞系HTR8/Svneo的CCL28的分泌。采用噻唑兰(MTT)、Annexin V-FITC、Transwell方法检测人工合成的CCL28对HTR8/Svneo细胞的增殖、凋亡及浸润能力的影响。RT-PCR及明胶酶谱法阐明HTR8/Svneo细胞浸润功能改变的机制。结果:HTR8/Svneo细胞与蜕膜基质细胞均能分泌CCL28,两者共培养能促进CCL28的分泌(P0.01)。人工合成的CCL28不影响滋养细胞的增殖(P0.05)和凋亡(P0.05),但能增强细胞的浸润能力(P0.01),且主要通过受体CCR10介导。RT-PCR与明胶酶谱法提示,CCL28通过提高滋养细胞内MMP-2活力来增强其浸润能力。结论:CCL28通过与受体CCR10的结合,以自分泌或旁分泌的方式增强滋养细胞MMP-2活力来促进滋养细胞的浸润功能。
[Abstract]:Objective: To explore the effect of chemokine CCL28 on the biological behavior of trophoblasts and its regulatory mechanism. Methods: Primary human decidual stromal cells (DSCs) of early pregnancy were isolated, cultured and identified. The secretion of CCL28 from human DSCs and human normal choriotrophoblastic cell line HTR8/Svneo was detected by ELISA. MTT, Annexin V-FITC and Transwell methods were used to detect the secretion of CCL28. The effects of synthetic CCL28 on the proliferation, apoptosis and infiltration of HTR8/Svneo cells were studied by RT-PCR and gelatin zymography. The mechanism of infiltration function of HTR8/Svneo cells was elucidated. Results: Both HTR8/Svneo cells and decidual stromal cells could secrete CCL28, and the co-culture of them could promote the secretion of CCL28 (P 0.01). Cell proliferation (P 0.05) and apoptosis (P 0.05), but it can enhance cell infiltration (P 0.01), and mainly through the receptor CCR10 mediated. RT-PCR and gelatin zymography suggest that CCL28 can enhance the infiltration ability of trophoblasts by increasing the activity of MMP-2. Conclusion: CCL28 can enhance trophoblasts by binding to the receptor CCR10 in the form of autocrine or paracrine. Cell MMP-2 activity promotes the infiltration of trophoblast cells.
【作者单位】：第四军医大学唐都医院妇产科;第四军医大学唐都医院传染科;
【基金】：国家自然科学基金(No:81471474) 唐都医院院创新基金(No:2015LCYJ013)
【分类号】：R714

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