PI3K-AKT信号通路在宫颈病变中的表达及其与HPV感染关系的研究
发布时间：2018-08-22 16:34
【摘要】：目的:磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)及处于其下游的蛋白激酶B(protein kinase B,Akt或PKB)组成的信号通路在人类肿瘤的发生发展演变过程中发挥重要作用,且现已证实宫颈癌的发生与人乳头瘤病毒(Human Papilloma Virus,HPV)的持续性感染相关。本研究旨在探讨PI3K、AKT在宫颈病变组织中的表达及其与HPV感染的相关性。方法:1、收集蚌埠医学院第一附属医院子宫颈活检及手术切除标本174例。其中正常宫颈组织30例、宫颈上皮内瘤变组织76例(LSIL组织18例,HSIL组织58例)、宫颈癌组织68例(I期36例、II期32例)。2、应用免疫组化SP法染色检测PI3K p110α、p-AKT蛋白在中的表达情况。3、同时应用杂交捕获(HC2)技术进行宫颈粘液脱落细胞HPV-DNA检测。4、采用SPSS17.0对数据进行处理,结果结合临床资料进行分析,应用c2检验,Mann-Whitney U检验,Spearman等级相关分析进行统计。P0.05定义为有统计学差异。结果:1、PI3K p110α蛋白在宫颈癌组的表达明显高于在LSIL、HSIL组(P0.05),其在HSIL组的表达也明显高于LSIL组(P0.01)。p-Akt蛋白在LSIL的表达明显低于在HSIL组及宫颈癌组中的表达(P0.01),而HSIL组与与宫颈癌组之间表达差异无统计学意义(P0.05)。2、PI3K p110α蛋白与p-Akt蛋白在病变宫颈组织中的表达具有明显的正相关性(P0.001)。3、PI3K p110α蛋白与p-Akt蛋白表达与HPV感染在各宫颈组织中的表达具有明显的正相关性(P0.05)。4、PI3K p110α、p-Akt的表达与宫颈癌FIGO分期、病理学分级以及淋巴结转移无关(P0.05)。结论:PI3K p110α、p-Akt表达及HPV感染在宫颈病变过程中密切相关,联合检测上述指标可能为宫颈病变的早期诊断提供依据,针对上述指标的靶向药物的应用可能为宫颈癌的治疗提供思路和方向。
[Abstract]:Objective: the signaling pathway composed of phosphoinositide 3-kinase PI3K and its downstream protein kinase B (protein kinase Bu Akt or PKB plays an important role in the development and development of human tumors. It has been confirmed that cervical cancer is associated with persistent infection of human papillomavirus (Human Papilloma). The purpose of this study was to investigate the expression of PI3 Knakt in cervical lesions and its correlation with HPV infection. Methods one hundred and forty four specimens of cervical biopsy and surgical excision were collected from the first affiliated Hospital of Bengbu Medical College. Among them, 30 cases were normal cervix tissues. There were 76 cases of cervical intraepithelial neoplasia (58 cases of LSIL) and 68 cases of cervical carcinoma (32 cases of stage I 36 cases of stage II) .2The expression of PI3K p110 伪 -p-AKT protein was detected by SP immunohistochemical staining, and the expression of p110 伪 -p-AKT protein was detected by hybridization capture. (HC2) technique was used to detect HPV-DNA of cervical mucus exfoliated cells. SPSS17.0 was used to process the data. Results according to the clinical data, using C2 test Mann-Whitney U test and Spearman grade correlation analysis, the statistical analysis was defined as statistical difference. Results the expression of PI3K p110 伪 protein in cervical cancer group was significantly higher than that in Lsil HSIL group (P0.05), and its expression in HSIL group was significantly higher than that in LSIL group (P0.01) .p-Akt protein expression in LSIL was significantly lower than that in HSIL group and cervical cancer group (P0.01), while that in HSIL group and cervical cancer group was significantly lower (P0.01). There was no significant difference between the expression of PI3K p110 伪 protein and p-Akt protein in cervical lesions (P0.05). (P0.001) .3PI3K p110 伪 protein and p-Akt protein expression and HPV infection in cervical tissues were significantly positive correlation (P0. 001). 3 PI3K p110 伪 protein expression and HPV infection in the cervix tissues, there was a significant positive correlation between the expression of PI3K p110 伪 protein and the expression of HPV protein in cervical tissues. The correlation between the expression of PI3K p110 伪 p-Akt and the FIGO stage of cervical cancer was observed. Pathological grading and lymph node metastasis were not significant (P0.05). Conclusion the expression of 10% PI3K p110 伪 p-Akt and HPV infection are closely related to the process of cervical lesions. The combined detection of these markers may provide evidence for the early diagnosis of cervical lesions. The application of targeted drugs for these indicators may provide ideas and directions for the treatment of cervical cancer.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33
本文编号：2197687
[Abstract]:Objective: the signaling pathway composed of phosphoinositide 3-kinase PI3K and its downstream protein kinase B (protein kinase Bu Akt or PKB plays an important role in the development and development of human tumors. It has been confirmed that cervical cancer is associated with persistent infection of human papillomavirus (Human Papilloma). The purpose of this study was to investigate the expression of PI3 Knakt in cervical lesions and its correlation with HPV infection. Methods one hundred and forty four specimens of cervical biopsy and surgical excision were collected from the first affiliated Hospital of Bengbu Medical College. Among them, 30 cases were normal cervix tissues. There were 76 cases of cervical intraepithelial neoplasia (58 cases of LSIL) and 68 cases of cervical carcinoma (32 cases of stage I 36 cases of stage II) .2The expression of PI3K p110 伪 -p-AKT protein was detected by SP immunohistochemical staining, and the expression of p110 伪 -p-AKT protein was detected by hybridization capture. (HC2) technique was used to detect HPV-DNA of cervical mucus exfoliated cells. SPSS17.0 was used to process the data. Results according to the clinical data, using C2 test Mann-Whitney U test and Spearman grade correlation analysis, the statistical analysis was defined as statistical difference. Results the expression of PI3K p110 伪 protein in cervical cancer group was significantly higher than that in Lsil HSIL group (P0.05), and its expression in HSIL group was significantly higher than that in LSIL group (P0.01) .p-Akt protein expression in LSIL was significantly lower than that in HSIL group and cervical cancer group (P0.01), while that in HSIL group and cervical cancer group was significantly lower (P0.01). There was no significant difference between the expression of PI3K p110 伪 protein and p-Akt protein in cervical lesions (P0.05). (P0.001) .3PI3K p110 伪 protein and p-Akt protein expression and HPV infection in cervical tissues were significantly positive correlation (P0. 001). 3 PI3K p110 伪 protein expression and HPV infection in the cervix tissues, there was a significant positive correlation between the expression of PI3K p110 伪 protein and the expression of HPV protein in cervical tissues. The correlation between the expression of PI3K p110 伪 p-Akt and the FIGO stage of cervical cancer was observed. Pathological grading and lymph node metastasis were not significant (P0.05). Conclusion the expression of 10% PI3K p110 伪 p-Akt and HPV infection are closely related to the process of cervical lesions. The combined detection of these markers may provide evidence for the early diagnosis of cervical lesions. The application of targeted drugs for these indicators may provide ideas and directions for the treatment of cervical cancer.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33
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