慢病毒介导靶向survivin基因的shRNA抑制子宫内膜在裸鼠腹腔种植生长

发布时间：2018-08-24 18:57

【摘要】：目的研究慢病毒介导生存素(survivin)基因特异性短发夹状RNA(sh RNA)对人子宫内膜在裸鼠腹腔种植生长的影响。方法将45只裸鼠随机分为实验组、阴性对照组及空白对照组,每组15只,将子宫内膜异位症患者在位内膜注射入裸鼠腹腔,同时将survivin-sh RNA慢病毒、空载慢病毒及磷酸盐缓冲液分别注射至3组裸鼠腹腔内,2周后观察各组子宫内膜种植成功率,光镜下观察异位病灶的形态学特点,免疫组化方法检测各组病灶中survivin的表达水平。结果实验组子宫内膜种植成功率(26.67%)明显低于阴性对照组(73.33%)及空白对照组(80%,P0.01);实验组成活的异位内膜腺体发育不良,间质伴有不同程度的坏死;该组病灶中survivin蛋白表达亦明显下降,与两对照组比较差异有显著性(P0.001)。结论慢病毒介导survivin基因特异性sh RNA能够通过靶向沉默survivin基因明显抑制人子宫内膜在裸鼠腹腔中的种植生长。
[Abstract]:Objective to study the effect of short hairpin RNA (sh RNA) mediated by lentivirus on the growth of human endometrium in nude mice. Methods 45 nude mice were randomly divided into experimental group, negative control group and blank control group with 15 in each group. The eutopic endometrium of endometriosis was injected into the abdominal cavity of nude mice, and survivin-sh RNA lentivirus was injected into the abdominal cavity of nude mice. The success rate of implantation of endometrium was observed 2 weeks after injection of lentivirus and phosphate buffer solution into the abdominal cavity of three groups of nude mice. The morphologic characteristics of ectopic lesions were observed under light microscope. The expression of survivin in the lesions was detected by immunohistochemical method. Results the success rate of endometrial implantation in the experimental group (26.67%) was significantly lower than that in the negative control group (73.33%) and the blank control group (P0.01). There was a significant difference between the two groups (P0.001). Conclusion Lentivirus mediated survivin gene specific sh RNA can inhibit the implantation of human endometrium in nude mice by targeting survivin gene.
【作者单位】：南方医科大学珠江医院妇产科;
【基金】：广东省科技计划项目(2011B031800089)
【分类号】：R711.71

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