妊娠期高血压疾病母胎界面中细胞间粘附因子-1和血管细胞粘附因子-1的表达和意义
发布时间：2018-08-27 07:06
【摘要】：研究背景及目的：妊娠期高血压疾病是妊娠期特发的疾病，是最常见孕期并发症，也孕产妇和围生儿死亡率升高的主要原因之一。但是其发病机制尚未完全阐明。细胞黏附分子(cell adhesionmolecule, CAM)在免疫应答、对移植物的排斥反应以及在血管内皮活化的过程中起着重要作用。很多研究表明，CAM与妊娠期高血压疾病的发病有着密切联系。本研究旨在研究母胎界面中2种重要的CAM分子：细胞间粘附因子-1（cell adhesion molecule-1, ICAM-1）和血管细胞粘附因子-1（vascular cell adhesion molecule-1, VCAM-1）在妊娠期高血压疾病发病中的作用，为妊娠期高血压疾病的预防和治疗提供科学依据。 材料和方法：收集10例正常的产妇、20例轻度子痫前期产妇、20例重度子痫前期产妇的胎盘及底蜕膜组织：采用免疫组织化学方法分别测定母胎界面（胎盘和底蜕膜）中ICAM-1和VCAM-1的表达，免疫组化完成之后，采用Olympus DP21显微镜进行图片采集，每张切片在400倍高倍镜下随机选择切片的5个视野。并采用图像分析系统(Image-pro plus5.0软件)测定图片累积光密度值（integraloptical density，IOD），取其平均值。然后采用SPSS18.0统计软件，，对各组的数据进行比较。采用方差分析的方法比较各组间两种因子表达的强度的差异.各组临床资料与ICAM-1和VCAM-1表达强度的相关性。 结论：1. ICAM-1和VCAM-1在正常妊娠、轻度子痫前期、重度子痫前期底蜕膜组织中均有表达；2. ICAM-1和VCAM-1在妊娠期高血压疾病胎盘组织中表达高于正常胎盘组织；其中重度子痫前期组胎盘组织中的ICAM-1和VCAM-1较轻度子痫前期组升高更明显。
[Abstract]:Background and objective: hypertensive disorder complicating pregnancy is one of the most common complications during pregnancy and one of the main reasons for the increase of maternal and perinatal mortality. However, its pathogenesis has not been fully elucidated. Cell adhesion molecule (cell adhesionmolecule, CAM) plays an important role in immune response, graft rejection and vascular endothelial activation. Many studies have shown that CAM is closely related to the pathogenesis of hypertensive disorders complicating pregnancy. The purpose of this study was to investigate the role of two important CAM molecules in maternal and fetal interface: intercellular adhesion factor-1 (cell adhesion molecule-1, ICAM-1 (ICAM-1) and vascular cell adhesion factor-1 (vascular cell adhesion molecule-1, VCAM-1 (VCAF-1) in the pathogenesis of hypertensive disorder complicating pregnancy. To provide scientific basis for the prevention and treatment of hypertensive disorder complicating pregnancy. Materials and methods: placenta and decidua of 20 women with severe preeclampsia were collected from 10 normal women with mild preeclampsia. The maternal and fetal interfaces (placenta and decidua) were measured by immunohistochemical method. For the expression of ICAM-1 and VCAM-1, After the completion of immunohistochemistry, the images were collected by Olympus DP21 microscope. Each slice was randomly selected for 5 visual fields under a 400-fold high-power microscope. The cumulative optical density (integraloptical density,IOD) of images was measured by image analysis system (Image-pro plus5.0), and the average value was calculated. Then SPSS18.0 statistical software was used to compare the data of each group. ANOVA was used to compare the intensity of expression of two kinds of factors in each group. The correlation between the clinical data and the expression of ICAM-1 and VCAM-1 in each group. Conclusion 1. ICAM-1 and VCAM-1 were expressed in decidua of normal pregnancy, mild preeclampsia and severe preeclampsia. The expression of ICAM-1 and VCAM-1 in placenta of hypertensive disorder complicating pregnancy was higher than that of normal placenta, and the ICAM-1 and VCAM-1 in placenta of severe preeclampsia were higher than those of mild preeclampsia.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.246
本文编号：2206499
[Abstract]:Background and objective: hypertensive disorder complicating pregnancy is one of the most common complications during pregnancy and one of the main reasons for the increase of maternal and perinatal mortality. However, its pathogenesis has not been fully elucidated. Cell adhesion molecule (cell adhesionmolecule, CAM) plays an important role in immune response, graft rejection and vascular endothelial activation. Many studies have shown that CAM is closely related to the pathogenesis of hypertensive disorders complicating pregnancy. The purpose of this study was to investigate the role of two important CAM molecules in maternal and fetal interface: intercellular adhesion factor-1 (cell adhesion molecule-1, ICAM-1 (ICAM-1) and vascular cell adhesion factor-1 (vascular cell adhesion molecule-1, VCAM-1 (VCAF-1) in the pathogenesis of hypertensive disorder complicating pregnancy. To provide scientific basis for the prevention and treatment of hypertensive disorder complicating pregnancy. Materials and methods: placenta and decidua of 20 women with severe preeclampsia were collected from 10 normal women with mild preeclampsia. The maternal and fetal interfaces (placenta and decidua) were measured by immunohistochemical method. For the expression of ICAM-1 and VCAM-1, After the completion of immunohistochemistry, the images were collected by Olympus DP21 microscope. Each slice was randomly selected for 5 visual fields under a 400-fold high-power microscope. The cumulative optical density (integraloptical density,IOD) of images was measured by image analysis system (Image-pro plus5.0), and the average value was calculated. Then SPSS18.0 statistical software was used to compare the data of each group. ANOVA was used to compare the intensity of expression of two kinds of factors in each group. The correlation between the clinical data and the expression of ICAM-1 and VCAM-1 in each group. Conclusion 1. ICAM-1 and VCAM-1 were expressed in decidua of normal pregnancy, mild preeclampsia and severe preeclampsia. The expression of ICAM-1 and VCAM-1 in placenta of hypertensive disorder complicating pregnancy was higher than that of normal placenta, and the ICAM-1 and VCAM-1 in placenta of severe preeclampsia were higher than those of mild preeclampsia.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.246
【参考文献】
相关期刊论文 前2条
1 王绍娟;VCAM-1、TNF-α、IL-6在妊娠期高血压疾病发病中的作用[J];中国妇产科临床杂志;2004年01期
2 蔡烟岫,耿淑平,刘曼华,谢晓谦;内皮细胞粘附分子在妊高征发病中的作用[J];南通医学院学报;2004年03期
本文编号：2206499
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