BKCa、SK3、TREK-1钾离子通道在晚孕大鼠子宫中的表达及功能研究
发布时间：2018-08-27 10:55
【摘要】：目的:探讨大电导钙激活钾通道(BKCa)、小电导钙激活钾通道(SK3)以及双孔钾离子通道(TREK-1)在非孕和晚孕大鼠子宫中的表达,以及对子宫舒缩功能的影响。方法:将SD大鼠分为非孕组(NP)和晚孕组(孕19天,LP),采用Western blot法和q PCR法检测非孕和晚孕大鼠子宫组织中3种收缩相关钾离子通道(BKCa、SK3、TREK-1)蛋白和基因表达水平。利用等长收缩实验,观察比较两组子宫肌条在缩宫素诱导下的收缩能力变化以及TREK-1通道抑制剂对晚孕组子宫肌条收缩能力的影响。结果:与非孕组比较,晚孕组子宫组织中3种收缩相关钾离子通道(BKCa、SK3、TREK-1)蛋白表达水平和基因表达水平显著提高(P0.05),晚孕组肌条在缩宫素诱导下的收缩力显著降低(P0.05)。TREK-1通道抑制剂L-甲硫氨酸可显著提高晚孕组子宫肌条收缩能力(P0.05)。结论:晚孕子宫静息状态的维持与BKCa、SK3以及TREK-1通道表达密切相关,TREK-1通道可能通过调节子宫收缩能力参与分娩发动。
[Abstract]:Aim: to investigate the expression of large conductance calcium activated potassium channel (BKCa), small conductance calcium activated potassium channel (SK3) and double pore potassium ion channel (TREK-1) in the uterus of non-pregnant and late pregnant rats, and their effects on the uterine function. Methods: SD rats were divided into non-pregnant group (NP) and late pregnancy group (19-day LP). Western blot and Q PCR methods were used to detect three kinds of contractile potassium channel (BKCa,SK3,TREK-1) protein and gene expression in the uterus of non-pregnant and late pregnant rats. The contractile ability of uterine muscle strips induced by oxytocin and the effect of TREK-1 channel inhibitor on the contractility of uterine muscle strips in late pregnancy group were observed and compared by isometric contraction experiment. Results: compared with the non-pregnant group, The expression of three contractile associated potassium channels (BKCa,SK3,TREK-1) protein and gene expression were significantly increased in the uterine tissues of the late pregnancy group (P0.05), and the contractile force of the muscle strips in the late pregnancy group was significantly decreased under oxytocin induction (P0.05). L- methionine, a channel inhibitor of TREK-1, was significantly decreased in the late pregnancy group (P0.05). The contractility of uterine muscle strips in late pregnancy group was significantly increased (P0.05). Conclusion: the maintenance of uterine resting state in late pregnancy is closely related to the expression of BKCa,SK3 and TREK-1 channels, and the TREK-1 channel may be involved in labor initiation by regulating uterine contractility.
【作者单位】： 安徽医科大学第一附属医院妇产科;
【基金】：国家自然科学基金青年科学基金(No:81300514) 中国博士后科学基金(No:2016M592039) 安徽省博士后研究人员科研活动基金(No:2015B080)
【分类号】：R714
，
本文编号：2207060
[Abstract]:Aim: to investigate the expression of large conductance calcium activated potassium channel (BKCa), small conductance calcium activated potassium channel (SK3) and double pore potassium ion channel (TREK-1) in the uterus of non-pregnant and late pregnant rats, and their effects on the uterine function. Methods: SD rats were divided into non-pregnant group (NP) and late pregnancy group (19-day LP). Western blot and Q PCR methods were used to detect three kinds of contractile potassium channel (BKCa,SK3,TREK-1) protein and gene expression in the uterus of non-pregnant and late pregnant rats. The contractile ability of uterine muscle strips induced by oxytocin and the effect of TREK-1 channel inhibitor on the contractility of uterine muscle strips in late pregnancy group were observed and compared by isometric contraction experiment. Results: compared with the non-pregnant group, The expression of three contractile associated potassium channels (BKCa,SK3,TREK-1) protein and gene expression were significantly increased in the uterine tissues of the late pregnancy group (P0.05), and the contractile force of the muscle strips in the late pregnancy group was significantly decreased under oxytocin induction (P0.05). L- methionine, a channel inhibitor of TREK-1, was significantly decreased in the late pregnancy group (P0.05). The contractility of uterine muscle strips in late pregnancy group was significantly increased (P0.05). Conclusion: the maintenance of uterine resting state in late pregnancy is closely related to the expression of BKCa,SK3 and TREK-1 channels, and the TREK-1 channel may be involved in labor initiation by regulating uterine contractility.
【作者单位】： 安徽医科大学第一附属医院妇产科;
【基金】：国家自然科学基金青年科学基金(No:81300514) 中国博士后科学基金(No:2016M592039) 安徽省博士后研究人员科研活动基金(No:2015B080)
【分类号】：R714
，
本文编号：2207060
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