孕乳期补充维生素D对仔鼠生长发育及细胞免疫功能的影响

发布时间：2018-08-27 12:40

【摘要】：目的研究孕乳期补充维生素D(VitD)对仔鼠生长发育及细胞免疫功能的影响。方法选用120只小鼠依据VitD治疗剂量不同分为高、中、低剂量组(各30只),分别给予VitD滴剂3.44、1.72、0.86IU,并设置对照组(30只)不给予VitD。观察幼鼠体质量、外周血25-羟维生素D_3[25-(OH)D_3],分别测定细胞免疫功能(迟发性变态反应实验)、体液免疫功能[包括抗体生成细胞数和半数溶血值(HC_(50))测定]、单核-巨噬细胞吞噬功能(包括小鼠腹腔巨噬细胞吞噬鸡红细胞实验和碳廓清实验),并进行T淋巴细胞亚群流式细胞术测定。结果随着VitD剂量的增加,子鼠外周血中25-(OH)D_3及钙离子水平逐渐升高,与对照组比较,差异均有统计学意义(P0.05);体质量及体长逐渐增加,各剂量组间比较,差异无统计学意义(P0.05)。与对照组比较,高剂量组、中剂量组足趾肿胀度、吞噬百分率、抗体生成细胞数、血清溶血素HC_(50)均增加,吞噬指数均减低,差异均有统计学意义(P0.05)。随VitD剂量的增加,CD4~+、CD8~+T淋巴细胞数量和CD4~+/CD8~+逐渐增加。结论孕乳期补充VitD可促进子代小鼠的生长发育,具有增强机体免疫功能的作用。
[Abstract]:Objective to study the effects of vitamin D (VitD) supplementation on growth and cellular immune function in neonatal rats. Methods 120 mice were divided into high, middle and low dose groups (30 rats each) according to the dosage of VitD. They were given VitD drops 3.44 (1.72) 0.86 IUU respectively, and the control group (30 mice) were not given VitD. the mice were divided into three groups: the control group (n = 30) and the control group (n = 30). Body mass of young rats was observed. Peripheral blood 25 hydroxyvitamin D 3 [25 (OH) D 3] was used to measure cellular immune function (delayed allergy test), humoral immune function [including the number of antibody producing cells and half hemolytic value (HC_ 50)], monocyte-macrophage phagocytosis (including phagocytosis of mononuclear macrophages). Mouse peritoneal macrophages phagocytosis of chicken red blood cells and carbon clearance test) and T lymphocyte subsets flow cytometry. Results with the increase of VitD dose, the levels of 25 (OH) D3 and Ca 2 + in the peripheral blood of the offspring increased gradually, the difference was statistically significant compared with the control group (P0.05), the body weight and body length increased gradually, and there was no significant difference between the two groups (P0.05). Compared with the control group, the toe swelling degree, phagocytosis percentage, the number of antibody forming cells, serum hemolysin HC_ _ (50), phagocytic index were increased and phagocytosis index were all decreased in high and middle dose groups (P0.05). With the increase of VitD dose, the number of CD4 ~ + CD8T lymphocytes and CD4~ / CD8 ~ + increased gradually. Conclusion supplementation of VitD during pregnancy and milk can promote the growth and development of offspring mice and enhance the immune function of the body.
【作者单位】：广东省清远市妇幼保健院科教科;暨南大学附属第一医院儿科;
【基金】：清远市产业技术研究与开发资金项目(2013A014)
【分类号】：R714

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