米非司酮对孕激素受体M阳性子宫肌瘤细胞增殖、凋亡的影响

发布时间：2018-08-27 16:38

【摘要】：目的观察米非司酮对孕激素受体M(PR-M)阳性子宫肌瘤细胞增殖、凋亡的影响。方法收集因子宫肌瘤行全子宫切除术患者的子宫肌瘤标本,原代培养子宫肌瘤细胞成功后,采用Western blotting法筛选出PR-M阳性细胞(阳性组)和PR-M阴性细胞(阴性组)。取对数生长期的两组细胞,分别加入0、1×10~(-6)、1×10~(-5)、1×10~(-4)mol/L的米非司酮,培养48 h后采用MTT法观察细胞增殖抑制情况,采用流式细胞仪观察细胞凋亡情况。结果米非司酮作用24 h后子宫肌瘤细胞生长明显减慢,细胞开始皱缩,48 h后细胞开始碎解。随米非司酮浓度增加,两组细胞增殖抑制率和凋亡率均逐渐增加(P均0.05)。当米非司酮浓度1×10-6mol/L时,阳性组细胞增殖抑制率和凋亡率低于阴性组(P均0.05)。结论米非司酮用药后PR-M阳性的子宫肌瘤细胞增殖受到抑制、凋亡增多,但与PR-M阴性细胞相比,增殖、凋亡变化程度较小。
[Abstract]:Objective to observe the effect of mifepristone on proliferation and apoptosis of uterine myoma cells positive for progesterone receptor M (PR-M). Methods the specimens of uterine leiomyoma from patients undergoing total hysterectomy were collected. After primary culture of uterine leiomyoma cells were successfully cultured, PR-M positive cells (positive group) and PR-M negative cells (negative group) were screened by Western blotting method. Two groups of cells in logarithmic growth stage were added with 1 脳 10 ~ (-5) ~ (-5) mol/L mifepristone respectively. After 48 hours of culture, the inhibition of cell proliferation was observed by MTT assay, and the apoptosis was observed by flow cytometry. Results after treated with mifepristone for 24 h, the growth of uterine leiomyoma cells decreased significantly, and the cells began to crinkle 48 hours later. With the increase of mifepristone concentration, the cell proliferation inhibition rate and apoptosis rate increased gradually in both groups (P 0.05). When mifepristone concentration was 1 脳 10-6mol/L, the cell proliferation inhibition rate and apoptosis rate in the positive group were lower than those in the negative group (all P 0. 05). Conclusion the proliferation and apoptosis of PR-M positive uterine myoma cells were inhibited and increased after mifepristone treatment, but the degree of proliferation and apoptosis was smaller than that of PR-M negative cells.
【作者单位】：郑州大学第三附属医院;
【基金】：河南省高等学校重点科研项目计划(16A320047)
【分类号】：R737.33

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