程序性死亡因子-1及其配体-1在子宫腺肌病中的表达及意义

发布时间：2018-08-27 20:22

【摘要】：目的探讨子宫腺肌病中程序性死亡因子-1(PD-1)及其配体-1(PD-L1)的表达,以及PD-1/PD-L1信号通路在子宫腺肌病发生发展中的作用。方法采用免疫组织化学法及Western blotting检测不同月经周期的子宫腺肌病在位内膜与异位子宫内膜、正常子宫内膜组织标本中PD-1、PD-L1蛋白的表达(每组增生期:n=15、分泌期:n=15)。分析不同组别间PD-1、PD-L1蛋白的表达差异及其与月经周期的关系。结果免疫组织化学结果显示,PD-1、PD-L1在不同月经周期的正常子宫内膜及腺肌病在位、异位子宫内膜中均有表达。腺肌病异位子宫内膜中PD-1蛋白(P0.01)与PD-L1蛋白(P0.01)表达升高。Western blotting结果显示,腺肌病在位子宫内膜中PD-1蛋白(P0.01)与PD-L1蛋白(P0.01)的表达增高。但在腺肌病组内不同月经周期中,PD-1/PD-L1的表达水平差异无统计学意义(P=0.12;P=0.45)。结论 PD-1蛋白及PD-L1蛋白在腺肌病在位及异位子宫内膜中的表达升高,提示PD-1/PD-L1信号通路可能参与子宫腺肌病的发生、发展过程。
[Abstract]:Objective to investigate the expression of programmed death factor 1 (PD-1) and its ligand 1 (PD-L1) in adenomyosis and the role of PD-1/PD-L1 signaling pathway in the development of adenomyosis. Methods Immunohistochemical method and Western blotting were used to detect the expression of PD-1,PD-L1 protein in eutopic endometrium and ectopic endometrium with different menstrual cycles. To analyze the difference of PD-1,PD-L1 protein expression in different groups and its relationship with menstrual cycle. Results Immunohistochemical results showed that PD-1 and PD-L1 were expressed in normal endometrium and adenomyosis in different menstrual cycle and ectopic endometrium. The expression of PD-1 protein (P0.01) and PD-L1 protein (P0.01) were increased in ectopic endometrium of adenomyosis. The results of Western blotting showed that the expression of PD-1 protein and PD-L1 protein in eutopic endometrium of adenomyosis was increased. However, there was no significant difference in the expression of PD-1 / PD-L1 in different menstrual cycles in patients with adenomyosis (P < 0. 12 or P < 0. 45). Conclusion the expression of PD-1 protein and PD-L1 protein in eutopic and ectopic endometrium of adenomyosis is increased, suggesting that PD-1/PD-L1 signaling pathway may be involved in the occurrence and development of adenomyosis.
【作者单位】：山东大学附属省立医院妇科;
【基金】：国家自然科学基金(81671433;81170549)
【分类号】：R711.71

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