卵母细胞玻璃化冷冻的临床结局分析

发布时间：2018-08-29 19:15

【摘要】：目的探讨卵母细胞玻璃化冷冻后解冻行体外受精-胚胎移植的临床结局。方法回顾性分析2010年1月至2014年10月北京大学第三医院生殖医学中心68例不孕症患者卵母细胞玻璃化冷冻后解冻行体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)的复苏率、2PN受精率、胚胎着床率、流产率和分娩率等临床结局。结果解冻玻璃化冻存的卵母细胞756枚,复苏率80.69%(610/756),其中MII卵母细胞519枚,2PN受精率53.56%(278/519)。68例患者中,12例无胚胎形成,占17.65%。56例患者共移植66个周期,其中新鲜胚胎移植51个周期,解冻胚胎移植15个周期。新鲜胚胎移植51个周期的临床妊娠率为54.90%(28/51),活胎分娩率为45.10%(23/51)。解冻胚胎移植15个周期的临床妊娠率为60.00%(9/15),活胎分娩率为46.67%(7/15)。累积妊娠率为55.88%(38/68)。每解冻一枚卵母细胞获得活胎分娩率为5.03%(38/756)。结论卵母细胞玻璃化冷冻技术在取卵日不能获得精子的患者中应用,可以获得良好的妊娠结局。
[Abstract]:Objective to investigate the clinical outcome of in vitro fertilization and embryo transfer after vitrification and thawing of oocytes. Methods from January 2010 to October 2014, the resuscitation rate and 2PN fertilization rate of 68 infertile oocytes from the Reproductive Medicine Center of the third Hospital of Peking University after vitrification and thawing for in vitro fertilization and embryo transfer (in vitro fertilization-embryo transfer,IVF-ET) were analyzed retrospectively. Embryo implantation rate, abortion rate and delivery rate, and other clinical outcomes. Results there were 756 oocytes in thawed vitrification, and the recovery rate was 80.69% (610 / 756). The fertilization rate of 519 MII oocytes was 53.56% (278 / 519). There was no embryogenesis in 12 of 68 patients, accounting for 17.65.56 of them, the fresh embryos were transferred for 51 cycles. Thawed embryos were transferred for 15 cycles. The clinical pregnancy rate of 51 cycles of fresh embryo transfer was 54.90% (28 / 51), and the delivery rate of live fetus was 45.10% (23 / 51). The clinical pregnancy rate was 60.00% (9 / 15) and the delivery rate of live fetus was 46.67% (7 / 15) in 15 cycles of thawed embryo transfer. The cumulative pregnancy rate was 55.88% (38 / 68). The rate of delivery of live fetus per thawed oocyte was 5.03% (38 / 756). Conclusion vitrified cryopreservation of oocytes can obtain good pregnancy outcome in patients who can not obtain sperm on the day of oocyte collection.
【作者单位】：北京大学第三医院生殖医学中心;
【基金】：首都卫生发展科研专项项目(首发2014-1-4091)
【分类号】：R714.8

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