人胎盘来源间充质干细胞对妊娠期高血压病细胞滋养细胞凋亡的影响:随机对照实验方案

发布时间：2018-09-01 16:16

【摘要】：背景:细胞滋养细胞凋亡增加、浸润能力下降是妊娠期高血压病发病的重要环节之一。人胎盘来源间充质干细胞已经在许多研究中显示出了对损伤的修复作用,而且胎盘间充质干细胞还具有一定的内分泌功能,可通过自分泌或旁分泌细胞因子的方式作用于近、远处的组织。因此,尝试移植人胎盘来源间充质干细胞修复损伤的滋养细胞功能,进而治疗妊娠期高血压病,有一定可行性。方法/设计:随机对照细胞学实验。收集人胎盘来源间充质干细胞培养液,过滤后即为人胎盘来源间充质干细胞条件培养基。培养人绒毛膜滋养层细胞系JEG-3细胞,随机分为3组,对照组在培养液中加入体积分数15%正常孕妇血清;妊娠期高血压病模型组在培养液中加入体积分数15%子痫前期重度患者的血清;人胎盘来源间充质干细胞条件培养组在培养液中加入体积分数15%子痫前期重度患者血清培养24 h后,更换为体积分数15%子痫前期重度患者血清+人胎盘来源间充质干细胞条件培养基。采用流式细胞仪检测各组细胞的凋亡率。讨论:实验从人胎盘来源间充质干细胞对妊娠期高血压病细胞滋养细胞功能的影响入手,可能为妊娠期高血压疾病的治疗找到一条细胞移植之路。伦理批准:研究方案取得沈阳医学院附属中心医院临床研究伦理委员会的书面批准。正常及子痫前期孕妇签署知情同意书。
[Abstract]:Background: the increase of cytotrophoblast apoptosis and the decrease of infiltrating ability are one of the important links in the pathogenesis of pregnancy-induced hypertension. Human placenta derived mesenchymal stem cells have been shown to repair damage in many studies, and placental mesenchymal stem cells also have endocrine function, which can be acted on by autocrine or paracrine cytokines. Distant tissue Therefore, it is feasible to transplant human placenta derived mesenchymal stem cells to repair the damaged trophoblastic function and to treat hypertensive disorder complicating pregnancy. Methods / Design: a randomized controlled cytological experiment. Human placenta derived mesenchymal stem cell culture medium was collected and filtered, which was then conditioned medium for human placenta derived mesenchymal stem cells. Human chorionic trophoblastic cell line JEG-3 cells were cultured and randomly divided into three groups. The control group added 15% normal maternal serum into the culture medium. The serum of 15% volume fraction of severe preeclampsia was added to the culture medium in the gestational hypertension model group. In the human placenta derived mesenchymal stem cell conditioned culture group, 15% volume fraction of serum was added to the culture medium for 24 h. The medium was replaced with 15% volume fraction of mesenchymal stem cells derived from human placenta in patients with severe preeclampsia. The apoptotic rate of each group was measured by flow cytometry. Discussion: based on the effect of human placenta derived mesenchymal stem cells on the function of trophoblast cells in hypertensive disorder complicating pregnancy, we may find a way of cell transplantation for the treatment of hypertensive disorder complicating pregnancy. Ethical approval: the research program has been approved by the Clinical Research Ethics Committee of the affiliated Central Hospital of Shenyang Medical College. Normal and preeclampsia pregnant women sign informed consent.
【作者单位】：沈阳市妇婴医院;沈阳医学院附属中心医院;
【分类号】：R714.246

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