戊酸雌二醇和黑升麻异丙醇提取物对卵巢切除小鼠的影响及其机制探讨

发布时间：2018-09-05 07:37

【摘要】：目的探究雌激素保护心血管的机制,评估黑升麻异丙醇提取物对卵巢切除小鼠各系统的影响,评估其安全性和有效性。方法选取C57BL/6小鼠及APOE-/-小鼠各20只,采用双侧卵巢切除法建立绝经动物模型。每种品系小鼠均分为4组：假手术组(SHAM+NS),单纯卵巢切除组(OVX+NS),卵巢切除并补充雌激素组(OVX+E2)和卵巢切除并补充黑升麻异丙醇提取物组(OVX+ICR)。分组干预12周,评估体重、血脂谱、主动脉、生殖系统及神经系统等。结果1.体重与脂肪分布：1) OVX+NS、OVX+ICR组体重大于SHAM+NS、OVX+E2组(P0.05)；2) OVX+NS、OVX+ICR组腹腔脂肪多于SHAM+NS、OVX+E2组;3) OVX+NS、OVX+ICR组胆固醇水平高于SHAM+NS、OVX+E2组。2.动脉粥样硬化斑块面积百分比：OVX+NS、OVX+ICR组动脉粥样硬化斑块面积百分比高于SHAM+NS、OVX+E2组(P0.05)。3.子宫重量：OVX+NS、OVX+ICR组子宫重量小于SHAM+NS、OVX+E2组(P0.05)。4.悬尾实验不动时间：OVX+NS组不动时间较SHAM+NS、OVX+E2、OVX+ICR组延长(P0.05)。结论1.补充雌二醇可以减轻卵巢切除小鼠体重,减少腹部脂肪,降低血脂水平,减小主动脉动脉粥样硬化斑块面积百分比,保护心血管系统；黑升麻异丙醇提取物在心血管系统保护方面无效。2.雌二醇对卵巢切除小鼠生殖系统具有促进增殖的作用；黑升麻异丙醇提取物应不具有这一作用。3.雌二醇和黑升麻异丙醇提取物可缩短卵巢切除小鼠悬尾实验的不动时间,改善精神状态。
[Abstract]:Objective to investigate the cardiovascular protection mechanism of estrogen, to evaluate the effects of isopropanol extract of black cohosh on ovariectomized mice, and to evaluate its safety and efficacy. Methods Twenty C57BL/6 mice and 20 APOE-/- mice were selected and bilateral ovariectomies were used to establish menopausal animal model. Each strain of mice was divided into four groups: sham-operated group, (SHAM NS), simple ovariectomized group, (OVX NS), ovariectomized with estrogen supplement group (OVX E2) and ovariectomized group supplemented with isopropanol extract group (OVX ICR). Body weight, blood lipid profile, aorta, reproductive system and nervous system were assessed after 12 weeks of intervention. Result 1. Weight and fat distribution) OVX NS,OVX ICR group was larger than SHAM NS,OVX E2 group (P0.05) OVX NS,OVX ICR group had more abdominal fat than SHAM NS,OVX E2 group (3) cholesterol level in OVX NS,OVX ICR group was higher than SHAM NS,OVX E2 group. The percentage of atherosclerotic plaque area in the NS,OVX ICR group was higher than that in the SHAM NS,OVX E2 group (P0.05). Uterine weight was smaller than that in SHAM NS,OVX E2 group (P0.05). The immobility time of OVX NS group was longer than that of SHAM NS,OVX E2OVX ICR group (P0.05). Conclusion 1. Estradiol supplementation can reduce the weight of ovariectomized mice, reduce abdominal fat, reduce the level of blood lipid, reduce the percentage of aortic atherosclerotic plaque area, and protect the cardiovascular system. The isopropanol extract of black cohosh has no effect on cardiovascular system protection. Estradiol could promote the proliferation of ovariectomized mouse reproductive system, but the extract of isopropanol did not. 3. Estradiol and Isopropanol extract could shorten the immobility time and improve the mental state of ovariectomized mice.
【学位授予单位】：北京协和医学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R711.75

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