单核细胞趋化蛋白-1对人卵巢癌CaOV3细胞黏附、侵袭能力的影响及其机制

发布时间：2018-09-05 19:06

【摘要】：目的探讨单核细胞趋化蛋白-1(MCP-1)对人卵巢癌Ca OV3细胞黏附、侵袭能力的影响及其机制。方法将对数生长期的人卵巢癌Ca OV3细胞随机分为观察组和对照组,观察组经MCP-1(20μg/m L)预处理,对照组不予处理。两组行体外黏附试验,记录黏附Fibronectin、Collagen及Laminin蛋白的细胞数;行体内黏附试验,记录黏附的细胞球体数目、体积及部位;行细胞侵袭试验,记录穿膜细胞数。采用Western blotting法检测两组上皮-间质转化指标(上皮指标E-cadherin,间质指标Vimentin、N-cadherin)的表达。结果观察组黏附Fibronectin、Collagen及Laminin蛋白的细胞数及穿膜细胞数均高于对照组,P均0.01。两组肿瘤细胞以细胞球体形式黏附在小鼠大网膜脂肪细胞部位,并且观察组黏附的细胞球体数目更多、球体更大。观察组E-cadherin低于对照组,Vimentin、Ncadherin均高于对照组,P均0.01。结论 MCP-1可提高人卵巢癌Ca OV3细胞的黏附、侵袭能力,可能与其促进上皮细胞间质转化有关。
[Abstract]:Objective to investigate the effect and mechanism of monocyte chemoattractant protein-1 (MCP-1) on adhesion and invasion of human ovarian cancer Ca OV3 cells. Methods Human ovarian cancer Ca OV3 cells in logarithmic phase were randomly divided into two groups: the observation group was pretreated with MCP-1 (20 渭 g / mL) and the control group was not treated. The number of cells attached to Fibronectin,Collagen and Laminin protein was recorded by in vitro adhesion test, the number, volume and location of adherent cells were recorded by in vivo adhesion test, and the number of perforated cells was recorded by cell invasion test. The expression of E-cadherin (Vimentin,N-cadherin) was detected by Western blotting method. Results the number of cells adhesion to Fibronectin,Collagen and Laminin protein and the number of perforated cells in the observation group were higher than those in the control group (P < 0.01). The tumor cells of the two groups were attached to the adipocytes of the greater omentum in the form of cytospheres, and the number of the cells in the observation group was more than that of the control group. The E-cadherin of the observation group was lower than that of the control group (P < 0.01). Conclusion MCP-1 can enhance the adhesion and invasion of human ovarian cancer Ca OV3 cells, which may be related to the promotion of epithelial interstitial transformation.
【作者单位】：麻城市人民医院;湖北省中南医院;
【分类号】：R737.31

【共引文献】