神经节苷脂GM3对人宫颈癌HeLa细胞凋亡和血管内皮生长因子表达的影响

发布时间：2018-09-09 10:30

【摘要】：目的研究外源性神经节苷脂GM3对人宫颈癌He La细胞增殖、凋亡和VEGF表达的影响。方法不同浓度GM3干预HeLa细胞24 h后,MTT法检测细胞增殖变化,流式细胞学技术检测细胞凋亡率,RT-PCR技术检测细胞VEGF mRNA表达水平,Western blot技术检测细胞VEGF蛋白水平。结果(1)GM3浓度分别为2、10、50和250μmol/L时,HeLa细胞生长抑制率分别为9.8%、32.9%、50.7%和78.6%,半数抑制浓度(IC50)为49.8μmol/L。(2)GM3浓度分别为10、50和250μmol/L时,HeLa细胞凋亡率分别为(4.9±0.4)%、(15.1±0.3)%、(24.4±0.7)%。(3)当GM3浓度高于10μmol/L时,HeLa细胞VEGF mRNA表达水平以及VEGF蛋白表达水平均显著下降(P0.05)。结论外源性神经节苷脂GM3能呈浓度依赖性抑制人宫颈癌He La细胞增殖,促进HeLa细胞凋亡,下调HeLa VEGF mRNA水平和蛋白水平,提示GM3可能通过调节肿瘤细胞凋亡和肿瘤血管生成而发挥双重抗肿瘤作用。
[Abstract]:Objective to study the effects of exogenous ganglioside GM3 on proliferation, apoptosis and VEGF expression of human cervical cancer He La cells. Methods the proliferation of HeLa cells was detected by MTT assay after 24 h intervention with different concentrations of GM3. Apoptosis rate was detected by flow cytometry and the expression level of VEGF mRNA was detected by RT-PCR. VEGF protein was detected by Western blot. Results (1) the growth inhibition rates of HeLa cells at GM3 concentration of 210U 50 and 250 渭 mol/L were 9.8and 32.9% and 78.6%, respectively. The half inhibitory concentration (IC50) was 49.8 渭 mol/L. (2) and the GM3 concentration was 109.50 渭 mol/L. (2) and 250 渭 mol/L, respectively. The apoptotic rate was (4.9 卤0.4), (15.1 卤0.3), (24.4 卤0.7) when GM3 concentration was higher than 10 渭 mol/L. (3) when GM3 concentration was higher than 10 渭 mol/L, the apoptotic rate of Hela cells was (4.9 卤0.4), (24.4 卤0.7), respectively. (3) when the GM3 concentration was higher than 10 渭 mol/L, the apoptotic rate was (4.9 卤0.4), (24.4 卤0.7), respectively. The level of VEGF protein expression decreased significantly (P0.05). Conclusion exogenous ganglioside GM3 can inhibit the proliferation of human cervical cancer He La cells in a concentration-dependent manner, promote the apoptosis of HeLa cells, and down-regulate the level of HeLa VEGF mRNA and protein. The results suggest that GM3 may play a dual anti-tumor effect by regulating tumor cell apoptosis and tumor angiogenesis.
【作者单位】：华中科技大学同济医学院附属协和医院妇产科;
【分类号】：R737.33

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