沙眼衣原体荧光PCR法在阴道炎患者实验室检测中的效果评价

发布时间：2018-09-12 14:17

【摘要】：目的评价沙眼衣原体(Chlamydia Trachomatis,CT)荧光PCR法在阴道炎患者实验室检测中的作用。方法选取闵行区中心医院妇科门诊760例有临床症状患者,采集其阴道分泌物进行白带常规检查、HE染色、细菌性阴道病(BV)检测,并运用荧光PCR法检测沙眼衣原体和解脲脲原体(Ureaplasma urealyticum,UU),并对CT进行相对定量分析。结果 CT阳性率14.87%,UU阳性率61.98%,显著高于真菌性阴道炎(7.24%)、滴虫性阴道炎(3.68%)及BV(10.39%),差异均有统计学意义(P0.05)。而且CT在性活跃人群(25岁)感染率最高。在排除UU、真菌感染、滴虫感染和BV后,妇科门诊患者根据白带常规和HE染色结果被分为阴道炎和非阴道炎两组,两组CT的感染率分别为14.29%和6.41%,差异具有统计学意义(P=0.040),CT拷贝对数值分别为5.88±0.99和4.79±1.17,两组具有统计学意义(P=0.025)。结论 CT是阴道炎重要病原菌之一,CT感染载量与阴道炎发生密切相关,应对妇科门诊及住院患者进行常规CT筛查。
[Abstract]:Objective to evaluate the role of chlamydia trachomatis (Chlamydia Trachomatis,CT) fluorescence PCR in laboratory detection of vaginitis. Methods A total of 760 patients with clinical symptoms were selected from gynecological outpatient department of Minhang Central Hospital and their vaginal secretions were collected for routine leucorrhea staining and (BV) detection for bacterial vaginosis. Chlamydia trachomatis and Ureaplasma Urealyticum (Ureaplasma urealyticum,UU) were detected by fluorescence PCR, and the relative quantitative analysis of CT was carried out. Results the positive rate of CT was significantly higher than that of fungal vaginitis (7.24%), trichomoniasis vaginitis (3.68%) and BV (10.39%) (P 0.05). And CT infection rates are highest in sexually active people (25 years old). After UU, fungal infection, trichomonad infection and BV were excluded, gynecological outpatients were divided into two groups according to leucorrhea routine and HE staining results: vaginitis group and non-vaginitis group. The infection rate of CT in the two groups was 14.29% and 6.41, respectively. The difference was statistically significant (P0. 040). The logarithmic values of CT copy were 5. 88 卤0. 99 and 4. 79 卤1. 17, respectively. The two groups had statistical significance (P0. 025). Conclusion CT is one of the most important pathogens of vaginitis. Ct infection load is closely related to the occurrence of vaginitis. Routine CT screening should be carried out in gynecological outpatients and inpatients.
【作者单位】：复旦大学附属闵行医院皮肤科;潍坊医学院;复旦大学附属闵行医院检验科;复旦大学附属华山医院检验医学科;新疆医科大学;复旦大学附属闵行医院输血科;
【基金】：上海市闵行区科委自然基金资助项目(2015MHZ003) 上海市闵行区卫计委基金资助项目(2014MW13) 上海市卫计委青年项目(20154Y0141)
【分类号】：R711.31

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