坤泰胶囊对大鼠实验性子宫异位内膜生长及其妊娠结局的影响

发布时间：2018-09-13 14:30

【摘要】：目的:研究坤泰胶囊(KTC)对SD大鼠实验性子宫异位内膜生长及其妊娠结局的影响。方法:手术法建立SD大鼠子宫内膜异位症(EMs)模型。将造模成功的SD大鼠分为模型对照组、阳性对照组(每只给予醋酸亮丙瑞林0.1 mg皮下注射,共1次),KTC低剂量组(每只按0.24 g/kg体质量灌胃,qd×21 d)、KTC高剂量组(每只按0.60 g/kg体质量灌胃,qd×21 d),每组14只。给药21 d后,每组解剖8只,比较给药前、后异位内膜体积变化;Western blotting法测定异位内膜caspase-8、caspase-3和caspase-9蛋白表达。每组剩余6只与雄鼠交配,妊娠12 d后,解剖观察受孕情况,并对胎盘、胎仔称量。动情周期正常的SD大鼠14只作为空白对照。结果:与模型对照组相比,KTC低、高剂量组体质量无统计学差异(P0.05);KTC低、高剂量组异位内膜抑制率分别为(40.25±35.18)%和(65.52±15.82)%;KTC低、高剂量组异位内膜中caspase-8、caspase-9和活化caspase-3明显升高(P0.05);KTC低剂量组的大鼠胎仔数量呈增加趋势。结论:KTC通过升高caspase-8、caspase-9和活化caspase-3蛋白含量,促进异位内膜的凋亡;KTC低剂量组EMs大鼠受孕率呈现出升高的趋势。
[Abstract]:Aim: to study the effects of Kuntai capsule (KTC) on ectopic endometrium growth and pregnancy outcome in SD rats. Methods: the (EMs) model of endometriosis in SD rats was established by operation. The successful SD rats were divided into the model control group and the positive control group. Each rat was subcutaneously injected with Leuprorelin Acetate 0.1 mg. A total of 1 dose of KTC was given in the low dose group (QD 脳 21 d). The high dose group (QD 脳 21 d),) was given QD 脳 21 d), at 0. 60 g/kg body weight in each group (n = 14, n = 10, n = 10). After 21 days of administration, 8 rats in each group were dissected, and the changes of ectopic intimal volume before and after administration were compared. The expression of caspase-8,caspase-3 and caspase-9 protein in ectopic endometrium was determined by Western blotting method. The remaining 6 rats in each group were mated with male mice. After 12 days of gestation, the pregnancy was observed and the placenta and fetus were weighed. Fourteen SD rats with normal estrous cycle were used as blank control. Results: compared with the model control group, KTC was lower in the high dose group than that in the model control group (P0.05). The inhibition rates of ectopic endometrium in the high dose group were (40.25 卤35.18)% and (65.52 卤15.82), respectively. Caspase-8,caspase-9 and activated caspase-3 in ectopic endometrium increased significantly in high dose group (P0.05). ConclusionKTC can promote apoptosis of ectopic endometrium by increasing the content of caspase-8,caspase-9 and activated caspase-3 protein. The pregnant rate of EMs rats in low dose group of KTC has a tendency to increase.
【作者单位】：广东医学院药学院;国家人口和计划生育委员会计划生育药具重点实验室上海市计划生育科学研究所生殖药理研究组;复旦大学;
【分类号】：R711.71

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