三氧化二砷联合塞来昔布对上皮性卵巢癌细胞的增殖抑制和凋亡诱导作用

发布时间：2018-09-17 18:17

【摘要】：目的探讨三氧化二砷(As2O3)联合塞来昔布(celecoxib)对上皮性卵巢癌细胞的增殖抑制和凋亡诱导作用及其机制。方法以上皮性卵巢癌细胞株(OVCAR-3)为研究对象,As2O3、celecoxib及两药联合作用于细胞株48 h后,噻唑蓝(MTT)法体外检测药物对细胞增殖抑制作用,并采用金正均q值法计算联合用药的q值。采用凋亡染色和流式细胞仪(FCM)检测药物对细胞凋亡和细胞周期影响,蛋白质印迹法(Western印迹)检测Bcl-2和caspase-3凋亡相关蛋白的表达水平。结果 As2O3和celecoxib对OVCAR-3细胞呈剂量依赖性的生长抑制作用,As2O3的半数抑制浓度(IC50)为4.00μmol/L,celecoxib的IC50为40.00μmol/L;As2O3和celecoxib联合用药后,OVCAR-3细胞的增殖抑制率和凋亡率均大于单药作用组(P0.05),联合用药的q值1.15,有显著的协同抗肿瘤效应。As2O3和celecoxib联合用药后OVCAR-3细胞发生明显的G2/M期阻滞,Bc1-2表达明显下调,而caspase-3表达则明显上调(P0.05)。结论 As2O3和celecoxib单药对OVCAR-3细胞的生长均有抑制作用,联合用药具有显著的协同作用。As2O3和celecoxib联合用药对于上皮性卵巢癌的药物治疗有重要的应用前景。
[Abstract]:Objective to investigate the effects of arsenic trioxide (As2O3) combined with celecoxib (celecoxib) on proliferation inhibition and apoptosis induction of epithelial ovarian cancer cells. Methods the cells of epithelial ovarian cancer cell line (OVCAR-3) were treated with as _ 2O _ 3 celecoxib and the two drugs for 48 h. The inhibitory effect of thiazolyl on cell proliferation was detected by thiazolyl (MTT) method in vitro, and the Q value of the combination drug was calculated by the Kim Jong un Q value method. Apoptosis staining and flow cytometry (FCM) were used to detect the effect of drugs on cell apoptosis and cell cycle. Western blot (Western blot) was used to detect the expression of Bcl-2 and caspase-3 apoptosis-related proteins. Results the inhibitory effect of As2O3 and celecoxib on the growth of OVCAR-3 cells in a dose-dependent manner (IC50 = 4.00 渭 mol/L,celecoxib, IC50 = 40.00 渭 mol/L;As2O3) and celecoxib combined treatment (P 0.05) showed that the proliferation inhibition rate and apoptosis rate of OVCAR-3 cells were higher than those of the control group (P0.05). The Q value of the drug was 1.15, and the expression of Bc1-2 was significantly down-regulated in OVCAR-3 cells after combined administration of as _ 2O _ 3 and celecoxib, and the expression of Bc1-2 was significantly down-regulated in G _ 2 / M phase arrest. The expression of caspase-3 was significantly up-regulated (P0.05). Conclusion both As2O3 and celecoxib have inhibitory effects on the growth of OVCAR-3 cells. The combination of as _ 2O _ 3 and celecoxib has significant synergistic effect on the treatment of epithelial ovarian cancer.
【作者单位】：南阳市中心医院肿瘤内二科;
【分类号】：R737.31

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