重度子痫前期胎盘组织中NFAT5表达与围生儿结局的相关性研究

发布时间：2018-09-18 16:35

【摘要】：目的:探讨核转录因子5(NFAT5)在重度子痫前期(sPE)胎盘中的表达及其与围生儿结局的相关性。方法:收集2015年1月至2016年1月在郑州大学第三附属医院行剖宫产分娩的sPE孕妇(试验组)60例。选取同期非PE行剖宫产术的孕妇60例作为对照组。免疫组化法检测胎盘组织中NFAT5表达;RT-PCR法检测胎盘组织中NFAT5 mRNA表达。对胎盘中NFAT5 mRNA水平与围生儿结局进行相关分析。结果:sPE组的新生儿体重、胎盘重量、Apgar评分及分娩孕周均低于对照组(P均0.05)。两组胎盘中NFAT5蛋白多位于胎盘滋养细胞的胞核与胞质中,绒毛间质细胞内有少量表达。sPE组的NFAT5蛋白和mRNA表达水平均高于对照组,差异均有统计学意义(t=-40.434,P=0.001;t=-6.696,P=0.001)。sPE组的NFAT5 mRNA相对表达量与新生儿出生体重、胎盘重量、分娩孕周均呈负相关(r分别为-0.554,-0.698,-0.612,P均0.05),与孕妇体重指数(BMI)呈正相关(r=0.630,P=0.012)。结论:sPE组胎盘组织中NFAT5表达水平升高,与围生儿不良结局有关。
[Abstract]:Aim: to investigate the expression of nuclear transcription factor 5 (NFAT5) in placenta of severe preeclampsia (sPE) and its correlation with perinatal outcome. Methods: 60 sPE pregnant women (trial group) who were delivered by cesarean section in the third affiliated Hospital of Zhengzhou University from January 2015 to January 2016 were collected. Sixty pregnant women with non-PE cesarean section were selected as control group. The expression of NFAT5 in placenta was detected by immunohistochemistry. RT-PCR was used to detect the expression of NFAT5 mRNA in placenta. The correlation between placental NFAT5 mRNA level and perinatal outcome was analyzed. Results the newborns weight, placental weight Apgar score and gestational week of delivery were significantly lower in the control group than in the control group (all P 0.05). The NFAT5 protein in placenta of both groups was mostly located in the nucleus and cytoplasm of placental trophoblast, and the expression of NFAT5 protein and mRNA in the villus interstitial cells was higher than that in the control group. The relative expression of NFAT5 mRNA was negatively correlated with birth weight, placental weight and gestational week of delivery (r = -0.554-0.698U -0.612P0.05, respectively), and positively correlated with (BMI) (r0.630P0.012). Conclusion the increased expression of NFAT5 in placental tissue of SPE group is related to perinatal adverse outcome.
【作者单位】： 郑州大学第三附属医院妇产科;
【基金】：河南省医学科技攻关项目(No:201503109)
【分类号】：R714.244
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