HPV16相关宫颈癌细胞系和组织中E7和DRR1蛋白的表达

发布时间：2018-09-19 20:29

【摘要】：目的:研究人乳头瘤病毒16(HPV16)相关宫颈癌细胞系和组织中E7和肾细胞癌下调基因1(DRR1)表达的关系。方法:以HPV16 E7阳性的宫颈癌Ca Ski和Si Ha细胞、HPV16 E7阴性的宫颈癌C-33A细胞及184例宫颈鳞状细胞癌组织为研究对象,采用Western blot方法和免疫组化染色方法检测E7和DRR1蛋白的表达,分析E7与DRR1蛋白表达的关系。结果:Western blot结果显示,C-33A细胞中HPV16 E7无表达,DRR1蛋白的表达显著高于Ca Ski细胞和Si Ha细胞(F=454.982,P0.001);Ca Ski细胞和Si Ha细胞表达HPV16 E7,二者DRR1蛋白的表达差异无统计学意义(P0.05)。免疫组化染色结果显示,HPV16 E7阳性宫颈鳞状细胞癌组织141例,其中DRR1蛋白阳性34例;HPV16 E7阴性宫颈鳞状细胞癌组织43例,其中DRR1蛋白阳性32例;HPV16 E7阳性组织中DRR1蛋白的表达显著低于HPV16 E7阴性组织(χ2=36.250,P0.001)。结论:DRR1可能是HPV16 E7作用的潜在靶基因。
[Abstract]:Aim: to investigate the relationship between E7 and DRR1 expression in human papillomavirus 16 (HPV16) associated cervical cancer cell lines and tissues. Methods: the expression of E7 and DRR1 protein was detected by Western blot and immunohistochemical staining in Ca Ski and Si Ha cells of cervix carcinoma with HPV16 E7 positive and HPV16E7 negative cervical carcinoma C-33A cells and 184 cases of cervical squamous cell carcinoma. To analyze the relationship between E7 and DRR1 protein expression. Results the results of blot showed that the expression of HPV16 E7 was significantly higher than that of Ca Ski cells and Si Ha cells (FF454.982, P0.001). The expression of HPV16 E7 in Ski cells and Si Ha cells was not significantly different from that in Ca Ski cells and Si Ha cells (P0.05). Immunohistochemical staining showed that there were 141 cases of squamous cell carcinoma of cervix with HPV16 E7 positive, of which 34 cases were positive for DRR1 protein, 43 cases were positive for HPV16 E7 negative cervical squamous cell carcinoma. The expression of DRR1 protein in 32 HPV16 E7 positive tissues was significantly lower than that in HPV16 E7 negative tissues (蠂 2 + 36.250 P 0.001). Conclusion: 1: DRR1 may be a potential target gene of HPV16 E7.
【作者单位】：新乡医学院病理学教研室;
【基金】：河南高校科技创新团队项目2012IRTSTHN015 河南省基础与前沿技术研究计划项目122300410121 河南省科技创新杰出青年基金资助项目094100510007 新乡医学院重点学科开放课题ZD200955
【分类号】：R737.33

【共引文献】