CIP2A和DAPK在宫颈癌中的表达及意义

发布时间：2018-10-05 17:34

【摘要】：目的：通过检测CIP2A与DAPK在宫颈癌组织中的表达情况，分析二者在宫颈癌发生发展过程中的意义及与临床病理因素的相关性。方法：1.收集2012年10月至2013年10月在泸州医学院附属医院妇产科手术切除的宫颈癌（cervical cancer，CC）病理组织标本38例，并取同期的宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）组织11例及正常宫颈组织（normal cervical epithelium，NCE）10例作为对照组。所有病人在取宫颈组织标本前均未接受过化疗或放疗，临床及病理资料完整。标本均经10%福尔马林固定，常规石蜡包埋，连续切片，片厚4μm，做HE染色和免疫组化染色。病理诊断均由有经验的病理科专家复核。三组病例的平均年龄分别为：正常宫颈组44.80±12.57岁，CIN组48.82±11.05岁，宫颈癌组45.47±7.31岁。2.用免疫组化Envision方法检测CIP2A、DAPK在正常宫颈、宫颈上皮内瘤变及宫颈癌组织中的表达情况。3.实验结果采用ImageproPlus5.0软件分析图片平均光密度（mean optical density，MOD），得出数据采用独立样本t检验，相关性分析采用Spearman等级相关分析，取用双侧检验，P＜0.05为有统计学意义。结果：1.CIP2A蛋白在不同宫颈组织中的阳性反应主要分布在细胞膜和（或）细胞质，CIP2A在正常宫颈、CIN、宫颈癌组织中测定的MOD值分别为10.27±18.19、46.71±39.23、61.96±39.06，总体随病变严重程度加重呈升高趋势，将之MOD值分别两两进行t检验，，结果：在正常宫颈与CIN中CIP2A的表达差异有统计学意义（P=0.015＜0.05），在正常宫颈与宫颈癌中CIP2A的表达差异有统计学意义（P＜0.001）;在CIN与宫颈癌中CIP2A的表达差异无统计学意义（P＞0.05）；在宫颈癌患者中，CIP2A的表达在不同浸润深度组中差异有统计学意义（P＜0.05），在不同肿瘤大小、不同组织类型、不同分化程度、不同临床分期、淋巴结转移阳性和阴性组、脉管内是否见癌栓组以及不同年龄组中表达差异无统计学意义（P＞0.05）。2.DAPK蛋白在正常宫颈组织中的阳性反应主要分布在细胞核和（或）细胞质，在CIN及宫颈癌组织中阳性反应主要在细胞膜和（或）细胞质；阳性反应均表现为不同程度的黄色或棕黄色着色。DAPK在正常宫颈、CIN、宫颈癌组中测定的MOD值进行K-S正态检验发现其不符合正态分布，将其进行开方转换后再次检验符合正态分布，转换后DAPK在正常宫颈、CIN、宫颈癌组中的MOD值依次为9.04±3.47、9.04±3.47、3.74±3.30，总体随病变严重程度加重呈降低趋势，将之分别两两进行t检验，结果：在正常宫颈与CIN中DAPK的表达差异无统计学意义（P＞0.05）；在正常宫颈与宫颈癌中DAPK的表达差异有统计学意义（P＜0.001）;在CIN与宫颈癌中DAPK的表达差异有统计学意义（P＜0.001）。在宫颈癌患者中，DAPK的表达在肿瘤最大径线≥4cm和＜4cm组表达差异有统计学意义（P=0.015＜0.05）。以35岁为界进行t检验，发现DAPK在≥35岁组与＜35岁组间表达差异有统计学意义（P=0.026＜0.05），在不同组织类型、不同分化程度、不同浸润深度、不同临床分期、淋巴结转移阳性和阴性组、脉管内是否见癌栓组中表达差异无统计学意义（P＞0.05）。3.二者的相关性：CIP2A与DAPK在宫颈组织中的表达强度无相关性（P＞0.05）。结论：1.CIP2A在宫颈癌中过表达，DAPK在宫颈癌中表达缺失（或减少）。2.CIP2A的表达增高和DAPK的表达降低可能与宫颈癌的发生有关。3.CIP2A和DAPK分别通过不同途径影响宫颈癌的发生发展，相互表达无相关性。
[Abstract]:Objective: To analyze the expression of CIP2A and DAPK in cervical cancer tissues, and to analyze the significance of both in the development of cervical cancer and the correlation with clinical pathological factors. Method: 1. 38 cases of cervical carcinoma (CC) were collected from October 2012 to October 2013 in the department of obstetrics and gynecology in Luzhou Medical College, and 11 cases of cervical intraepithelial neoplasia (CIN) and normal cervical tissue were taken. NCE) 10 cases were treated as control group. All patients had not received chemotherapy or radiotherapy before taking cervical tissue specimens, and the clinical and pathological data were complete. The specimens were fixed with 10% formalin, conventional paraffin embedding, continuous slicing, thickness of 4. m u.m, HE staining and immunohistochemical staining. Pathological diagnosis was reviewed by experienced pathologist. The mean age of the three cases were: normal cervical group (44. 80) 12. 57 years old, CIN group (48. 82) 11. 05 years old, cervical cancer group (45. 47) 7. 31 years old. The expression of CIP2A and DAPK in normal cervical, cervical intraepithelial neoplasia and cervical carcinoma were detected by immunohistochemistry. The average optical density (MOD) of image was analyzed by ImageptroPlus5.0 software. The data was tested by independent sample t. The correlation analysis was analyzed by Spearman grade, and the two-side test was taken, and P <0.05 was statistical significance. Results: 1. The positive reaction of CIP2A protein in different cervical tissues was mainly distributed in cell membrane and (or) cytoplasm. The MOD values measured in the tissues of normal cervix, CIN and cervical carcinoma were 10.27, 18. 19, 46. 71, 39. 23, 61. 96 and 39. 06, respectively. The results showed that there was a significant difference in the expression of CIP2A between normal cervix and CIN (P = 0.0015 <0.05), and the expression of CIP2A in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was no significant difference in the expression of CIP2A between CIN and cervical carcinoma (P> 0.05). In patients with cervical cancer, the expression of CIP2A was statistically significant (P <0.05). There was no significant difference in the expression of DAPK in normal cervical tissues (P> 0.05). The positive reaction of DAPK protein in normal cervical tissues was mainly distributed in nucleus and/ or cytoplasm. In CIN and cervical carcinoma tissues, the positive reaction was mainly in the cell membrane and (or) cytoplasm; the positive reaction showed a different degree of yellow or brown yellow coloration. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was determined by K-S positive state test. It was found that it was not in accordance with normal distribution, and the normal distribution was again tested after it was switched on. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was 9.04, 3.47, 9.04, 3.47, 3.74, 3.30, respectively. Results: There was no significant difference in the expression of DAPK in normal cervix and CIN (P> 0.05), and the difference of DAPK expression in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was significant difference in the expression of DAPK in CIN and cervical carcinoma (P <0.001). In patients with cervical cancer, the expression of DAPK was statistically significant (P = 0. 015 <0.05). At the age of 35, the expression of DAPK in the 35-year-old group and <35-year-old group was statistically significant (P = 0. 0. 05). In different tissue types, different differentiation degrees, different infiltration depths, different clinical stages, lymph node metastasis and negative group, There was no significant difference in the expression of tumor thrombus in the vessel (P> 0.05). There was no correlation between the expression intensity of CIP2A and DIAPK in cervical tissues (P> 0.05). Conclusion: 1. CIP2A has been expressed in cervical carcinoma. The expression of DIAPK in cervical carcinoma is absent (or decreased). The expression of CIP2A and the decrease of DAPK may be related to the occurrence of cervical cancer.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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