Exenatide对多囊卵巢综合征大鼠胰腺组织SIRT1表达的影响

发布时间：2018-10-08 17:04

【摘要】：【目的】观察Exenatide对多囊卵巢综合征(PCOS)大鼠胰腺沉默信息调节因子1(SIRT1)表达的影响,探讨其对PCOS大鼠的干预治疗效果及可能机制。【方法】采用脱氢表雄酮(DHEA)诱导建立(n=50)PCOS雌性大鼠模型,随机分成Exenatide组(EX组,10只),给予EX颈部皮下注射;二甲双胍组(MF组,10只),给予MF无菌蒸馏水溶解灌胃;PCOS组(9只)及正常对照组(NC组,10只)。连续给药4周后检测各组大鼠血清空腹胰岛素(FINS)、并进行糖耐量试验(OGTT),计算HOMAIR指数。采用免疫组化及Real-time PCR法测定各组大鼠胰腺SIRT1的表达情况。【结果】1免疫组化和Q-PCR在蛋白水平和m RNA水平表明SIRT1表达于正常的胰腺内分泌组织中。PCOS组与NC组相比SIRT1表达明显降低(P0.05),经过药物干预后的EX和MF组与PCOS组相比表达均有不同程度升高(P0.05),而EX和MF之间相比没有统计学差异。2SIRT1表达与血清FPG、FINS、HOMA-IR呈明显负相关。【结论】Exenatide的干预改善了大鼠的血糖水平和胰岛素抵抗状态。SIRT1的表达下降可能在PCOS的发生发展中具有重要作用,Exenatide可能会通过上调SITR1的表达具有治疗PCOS的潜在作用。
[Abstract]:[objective] to observe the effect of Exenatide on the expression of pancreatic silencing information regulatory factor 1 (SIRT1) in (PCOS) rats with polycystic ovary syndrome (PCOS). [methods] female PCOS rats were induced by dehydroepiandrosterone (DHEA) and were randomly divided into Exenatide group (EX group, n = 10) and EX subcutaneously injected into the neck. Metformin group (MF group, n = 10) was given MF aseptic distilled water solution and intragastric perfusion (n = 9) and normal control group (NC group, n = 10). Fasting insulin (FINS), was measured after 4 weeks of continuous administration and HOMAIR index was calculated by glucose tolerance test (OGTT),). Immunohistochemical and Real-time PCR methods were used to detect the expression of SIRT1 in the pancreas of rats. [results] 1 the expression of SIRT1 in the normal pancreatic endocrine tissues was detected by immunohistochemistry and Q-PCR at the protein level and m RNA level. [results] 1 compared with the NC group, the expression of SIRT1 in the normal pancreatic endocrine tissues was detected. The expression of SIRT1 was significantly decreased (P0.05). After drug intervention, the expression of EX and MF was significantly higher than that of PCOS (P0.05). However, there was no significant difference between EX and MF in the expression of SIRT1. The expression of SIRT1 was negatively correlated with serum FPG,FINS,HOMA-IR. [conclusion] The decrease of insulin resistance. SIRT1 expression may play an important role in the pathogenesis and development of PCOS. Exenatide may play a potential role in the treatment of PCOS by upregulating the expression of SITR1.
【作者单位】：中山大学附属第三医院生殖医学中心;中山大学附属第三医院不育与性医学科;
【基金】：广东省科技计划项目(201013031600084;2013B022000012)
【分类号】：R711.75

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